53-Dependent Adaptive Responses in Human Cells Exposed to Space Radiations

Takahashi, Akio; Su, Xiaoming; Suzuki, Hiromi; Omori, Keisuke; Seki, Masayuki; Hashizume, Tōkō; Shimazu, Toru; Ishioka, Noriaki; Iwasaki, Takanori; Ohnishi, Takeo

doi:10.1016/j.ijrobp.2010.04.062

Cited by 15 publications

(6 citation statements)

References 30 publications

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“…The data obtained here are in agreement with recent reports that the stress-responsive activator of p300 stimulates the transcription of HSP genes through p53 (Barlev et al, 2001;Espinosa and Emerson, 2001;Xu et al, 2008). In this spaceflight experiment, it was found that the adaptive response was present only in space-flown wtp53 cells (Takahashi et al, 2010a). HSPs might be involved in possible mechanisms associated with the p53-dependent radioadaptive response (Takahashi and Ohnishi, 2009), because p53 represses the activity of the Hsp70 promoter via interactions with the CAAT binding factor (Agoff et al, 1993).…”

Section: Msh2supporting

confidence: 91%

Expression of p53-regulated genes in human cultured lymphoblastoid TSCE5 and WTK1 cell lines after spaceflight in a frozen state

Takahashi

Suzuki

Omori

et al. 2011

Advances in Space Research

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Section: Msh2supporting

confidence: 91%

Expression of p53-regulated genes in human cultured lymphoblastoid TSCE5 and WTK1 cell lines after spaceflight in a frozen state

Takahashi

Suzuki

Omori

et al. 2011

Advances in Space Research

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“…The possible mechanisms of the adaptive response include the transcription and translation of DNA repair-regulated and cell cycle-regulated genes [40,41], the activation of poly (ADP-ribose) polymerase [42], protein kinase C [43,44], p53 [45,46], and signal transducer and activator of transcription1 [47], and the secretion of nuclear clusterin [48]. Although radioadaptation was assumed to occur only when cells were exposed to a priming dose of low-LET, but not high-LET, irradiation [49], both low-LET and high-LET irradiation have been reported to act as challenging doses for radioadaptation.…”

Section: Adaptive Responsementioning

confidence: 99%

Role of High-Linear Energy Transfer Radiobiology in Space Radiation Exposure Risks

Takahashi

Ikeda

Yoshida

2018

International Journal of Particle Therapy

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Many manned missions to the Moon and Mars are scheduled in the near future. However, space radiation presents a major hazard to humans, and astronauts are constantly exposed to radiation, including high linear energy transfer (LET) radiation, which differs from radiation on Earth. Thus, there is thus an urgent need to clarify the biological effects of space radiation and reduce the associated risks. In this review, we consider the role of high-LET radiobiology in relation to space-radiation exposure.

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“…After space flight, the cells were cultured for 6 h and then irradiated with a challenging dose of 3 Gy. Thereafter, the cells were analyzed for indicators of the adaptive response [ 8 ]. Red columns, WTK1 (m p53 ); blue columns, TSCE5 (wt p53 ).…”

Section: Report On the Rad Gene Projectmentioning

confidence: 99%

Life science experiments performed in space in the ISS/Kibo facility and future research plans

Ohnishi

2016

J Radiat Res

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Over the past several years, current techniques in molecular biology have been used to perform experiments in space, focusing on the nature and effects of space radiation. In the Japanese ‘Kibo’ facility in the International Space Station (ISS), the Japan Aerospace Exploration Agency (JAXA) has performed five life science experiments since 2009, and two additional experiments are currently in progress. The first life science experiment in space was the ‘Rad Gene’ project, which utilized two human cultured lymphoblastoid cell lines containing a mutated p53 gene (mp53) and a parental wild-type p53 gene (wtp53) respectively. Four parameters were examined: (i) detecting space radiation–induced DSBs by observing γH2AX foci; (ii) observing p53-dependent gene expression during space flight; (iii) observing p53-dependent gene expression after space flight; and (iv) observing the adaptive response in the two cell lines containing the mutated and wild type p53 genes after exposure to space radiation. These observations were completed and have been reported, and this paper is a review of these experiments. In addition, recent new information from space-based experiments involving radiation biology is presented here. These experiments involve human cultured cells, silkworm eggs, mouse embryonic stem cells and mouse eggs in various experiments designed by other principal investigators in the ISS/Kibo. The progress of Japanese science groups involved in these space experiments together with JAXA are also discussed here. The Japanese Society for Biological Sciences in Space (JSBSS), the Utilization Committee of Space Environment Science (UCSES) and the Science Council of Japan (ACJ) have supported these new projects and new experimental facilities in ISS/Kibo. Currently, these organizations are proposing new experiments for the ISS through 2024.

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53-Dependent Adaptive Responses in Human Cells Exposed to Space Radiations

Cited by 15 publications

References 30 publications

Expression of p53-regulated genes in human cultured lymphoblastoid TSCE5 and WTK1 cell lines after spaceflight in a frozen state

Expression of p53-regulated genes in human cultured lymphoblastoid TSCE5 and WTK1 cell lines after spaceflight in a frozen state

Role of High-Linear Energy Transfer Radiobiology in Space Radiation Exposure Risks

Life science experiments performed in space in the ISS/Kibo facility and future research plans

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