6,6′-Bieckol Isolated from Ecklonia cava Protects Oxidative Stress Through Inhibiting Expression of ROS and Proinflammatory Enzymes in High-Glucose-Induced Human Umbilical Vein Endothelial Cells

Park, Mi‐Hwa; Heo, Soo‐Jin; Park, Pyo-Jam; Moon, Sang-Ho; Sung, Sangkyung; Jeon, Byong-Tae; Lee, Seung‐Hong

doi:10.1007/s12010-014-1099-4

Cited by 33 publications

(14 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…COX2 and PGE2 are important mediators of inflammation in endothelial cells (Li and Shah 2004). Recent studies have shown that certain phytochemicals attenuate high-glucose-or cytokine-induced increases in COX2 and PGE2 levels in human umbilical vein endothelial cells (Abbasi et al 2014;Chao et al 2011;Park et al 2014;Sheu et al 2008). In the present study, CZE attenuated the dRib-induced production of the inflammatory mediators COX2 and PGE2.…”

Section: Resultssupporting

confidence: 60%

Extracts of Chrysanthemum zawadskii attenuate oxidative damage to vascular endothelial cells caused by a highly reducing sugar

Kim

2017

Cytotechnology

View full text Add to dashboard Cite

Endothelial cells are considered candidates for involvement in the pathogenesis of diabetic vascular complications, and prevention of endothelial cell damage may be important in pharmacological attempts to prevent such complications. In the present study, I explored whether extracts of Chrysanthemum zawadskii (CZE) could prevent oxidative damage and dysfunction of a vascular endothelial cell line caused by the highly reducing sugar, 2-deoxy-D-ribose (dRib), and dysfunction of a vascular endothelial cell line. Vascular endothelial cells were treated with dRib in the presence or absence of CZE. Cell viability was monitored using a cell counting kit, and the induction of apoptosis was evaluated with a cell death kit. Prostaglandin E2 and cyclooxygenase-2 levels were measured using enzyme-linked immunosorbent assay kits. Mitochondrial membrane potential [ΔΨ(m)] was determined using a JC-1 kit. Intracellular oxidative stress was measured by fluorometric analysis of dichlorofluorescin oxidation using 2',7'-dichlorofluorescin diacetate as the probe. The expression levels of genes encoding antioxidant enzymes were analyzed by real-time polymerase chain reaction. dRib reduced cell survival and the ΔΨ(m) and markedly increased intracellular levels of reactive oxygen species and apoptosis. However, pretreatment of cells with CZE attenuated all these dRib-induced effects. The anti-oxidant N-acetyl-L-cysteine (NAC) also prevented dRib-induced oxidative cell damage. CZE attenuated the dRib-induced production of the inflammatory mediators cyclooxygenase-2 and Prostaglandin E2. NAC also exhibited anti-inflammatory effects and treatment with CZE caused transcriptional elevation of genes encoding antioxidant enzymes. Taken together, the results suggest that CZE may exert an antioxidant action that reduces dRib-induced cell damage to vascular endothelial cells and may thus aid in preventing diabetes-associated microvascular complications.

show abstract

Section: Resultssupporting

confidence: 60%

Extracts of Chrysanthemum zawadskii attenuate oxidative damage to vascular endothelial cells caused by a highly reducing sugar

Kim

2017

Cytotechnology

View full text Add to dashboard Cite

show abstract

“…Our recent study has demonstrated that the antisenescence effect of calcium-channel blockers in human endothelial cells is associated with increased eNOS activity [ 8 ], implying that eNOS activation is important in the regulation of the senescence program in endothelial cells by NO-mediated delay of cellular senescence [ 2 ]. The activity of eNOS is regulated by reciprocal phosphorylation of the activating site Ser-1177 and inhibiting site Thr-495, which affects NO bioavailability [ 33 ]. We found that both constant and intermittent glucose led to no changes in eNOS total expression, Ser-1177 eNOS phosphorylation, Thr-495 eNOS dephosphorylation, or NO release from control levels in HUVECs.…”

Section: Discussionmentioning

confidence: 99%

Intermittent High Glucose Implements Stress-Induced Senescence in Human Vascular Endothelial Cells: Role of Superoxide Production by NADPH Oxidase

et al. 2015

View full text Add to dashboard Cite

Impaired glucose tolerance characterized by postprandial hyperglycemia, which occurs frequently in elderly persons and represents an important preliminary step in diabetes mellitus, poses an independent risk factor for the development of atherosclerosis. Endothelial cellular senescence is reported to precede atherosclerosis. We reported that continuous high glucose stimulus causes endothelial senescence more markedly than hypertension or dyslipidemia stimulus. In the present study, we evaluated the effect of fluctuating glucose levels on human endothelial senescence. Constant high glucose increased senescence-associated-β-galactosidase(SA-β-gal) activity, a widely used marker for cellular senescence. Interestingly, in intermittent high glucose, this effect was more pronounced as well as increase of p21 and p16INK4a , senescence related proteins with DNA damage. However, telomerase was not activated and telomere length was not shortened, thus stress-induced senescence was shown. However, constant high glucose activated telomerase and shortened telomere length, which suggested replicative senescence. Intermittent but not constant high glucose strikingly up-regulated the expression of p22phox, an NADPH oxidase component, increasing superoxide. The small interfering RNA of p22phox undermined the increase in SA-β-gal activity induced by intermittent high glucose. Conclusively, intermittent high glucose can promote vascular endothelial senescence more than constant high glucose, which is in partially dependent on superoxide overproduction.

show abstract

“…The 6,6 -bieckol compound isolated from E. cava demonstrated an inhibition for the high-glucose-induced cytotoxicity in human umbilical vein endothelial cells (HUVECs) and insulinoma cells, showing it can be a potential therapeutic agent against the hyperglycemia-induced oxidative stress. This problem results in diabetic endothelial dysfunction [219,220].…”

Section: Phlorotanninsmentioning

confidence: 99%

Seaweed Phenolics: From Extraction to Applications

et al. 2020

View full text Add to dashboard Cite

Seaweeds have attracted high interest in recent years due to their chemical and bioactive properties to find new molecules with valuable applications for humankind. Phenolic compounds are the group of metabolites with the most structural variation and the highest content in seaweeds. The most researched seaweed polyphenol class is the phlorotannins, which are specifically synthesized by brown seaweeds, but there are other polyphenolic compounds, such as bromophenols, flavonoids, phenolic terpenoids, and mycosporine-like amino acids. The compounds already discovered and characterized demonstrate a full range of bioactivities and potential future applications in various industrial sectors. This review focuses on the extraction, purification, and future applications of seaweed phenolic compounds based on the bioactive properties described in the literature. It also intends to provide a comprehensive insight into the phenolic compounds in seaweed.

show abstract

6,6′-Bieckol Isolated from Ecklonia cava Protects Oxidative Stress Through Inhibiting Expression of ROS and Proinflammatory Enzymes in High-Glucose-Induced Human Umbilical Vein Endothelial Cells

Cited by 33 publications

References 33 publications

Extracts of Chrysanthemum zawadskii attenuate oxidative damage to vascular endothelial cells caused by a highly reducing sugar

Extracts of Chrysanthemum zawadskii attenuate oxidative damage to vascular endothelial cells caused by a highly reducing sugar

Intermittent High Glucose Implements Stress-Induced Senescence in Human Vascular Endothelial Cells: Role of Superoxide Production by NADPH Oxidase

Seaweed Phenolics: From Extraction to Applications

Contact Info

Product

Resources

About