Untitled

Snellinx, Zita

doi:10.1023/a:1023539104747

Cited by 56 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several microbial strains able to use DNT as the sole carbon (17,18) or nitrogen (19,20) source were reported. DNT can serve as a potential nitrogen source through the release of either nitrite or ammonium during oxidative or reductive biotransformation, respectively (16).…”

mentioning

confidence: 99%

Aerobic Transformation of 2,4-Dinitrotoluene by Escherichia coli and Its Implications for the Detection of Trace Explosives

Shemer

Yagur‐Kroll

Hazan

et al. 2018

Appl Environ Microbiol

View full text Add to dashboard Cite

DNT (2,4-dinitrotoluene), a volatile impurity in military-grade 2,4,6-trinitrotoluene (TNT)-based explosives, is a potential tracer for the detection of buried landmines and other explosive devices. We have previously described an bioreporter strain engineered to detect traces of DNT and have demonstrated that the gene promoter, the sensing element of this bioreporter, is induced not by DNT but by at least one of its transformation products. In the present study, we have characterized the initial stages of DNT biotransformation in , have identified the key metabolic products in this reductive pathway, and demonstrate that the main DNT metabolite that induces is 2,4,5-trihydroxytoluene. We further show that cannot utilize DNT as a sole carbon or nitrogen source and propose that this compound is metabolized in order to neutralize its toxicity to the cells. The information provided in this article sheds new light both on the microbial biodegradability of nitroaromatic compounds and on the metabolic capabilities of By doing so, it also clarifies the pathway leading to the previously unexplained induction of the gene by 2,4-dinitrotoluene, an impurity that accompanies 2,4,6-trinitrotoluene (TNT)-based explosives. Our improved understanding of these processes will serve to molecularly enhance the performance of a previously described microbial bioreporter of buried landmines and other explosive devices, in which the gene promoter serves as the sensing element.

show abstract

mentioning

confidence: 99%

Aerobic Transformation of 2,4-Dinitrotoluene by Escherichia coli and Its Implications for the Detection of Trace Explosives

Shemer

Yagur‐Kroll

Hazan

et al. 2018

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…The diverse metabolic capabilities have supported the Variovorax paradoxus to degrade a wide range of natural and xenobiotic compounds [34]. For instance, Snellinx et al [42] reported that the Variovorax paradoxus strain VM685 is capable of degrading 2,4-dinitrotoluene (2,4-DNT) and can coexist with other 2,4-DNT-degrading strain Pseudomonas sp. VM908.…”

Section: Discussionmentioning

confidence: 99%

Enrichment and Analysis of Stable 1,4-dioxane-Degrading Microbial Consortia Consisting of Novel Dioxane-Degraders

et al. 2019

View full text Add to dashboard Cite

Biodegradation of 1,4-dioxane, a water contaminant of emerging concern, has drawn substantial attention over the last two decades. A number of dioxane-degraders have been identified, though many of them are unable to metabolically utilize 1,4-dioxane. Moreover, it is considered more preferable to use microbial consortia rather than the pure strains, especially in conventional bioreactors for industrial wastewater treatment. In the present study, a stable 1,4-dioxane-degrading microbial consortium was enriched, namely 112, from industrial wastewater by nitrate mineral salt medium (NMSM). The consortium 112 is capable of utilizing 1,4-dioxane as a sole carbon and energy source, and can completely degrade 1,4-dioxane up to 100 mg/L. From the consortium 112, two 1,4-dioxane-degrading bacterial strains were isolated and identified, in which the Variovorax sp. TS13 was found to be a novel 1,4-dioxane-degrader that can utilize 100 mg/L of 1,4-dioxane. The efficacy of the consortium 112 was increased significantly when we cultured the consortium with mineral salt medium (MSM). The new consortium, N112, could utilize 1,4-dioxane at a rate of 1.67 mg/L·h. The results of the ribosomal RNA intergenic spacer analysis (RISA) depicted that changes in the microbial community structure of consortium 112 was the reason behind the improved degradation efficiency of consortium N112, which was exhibited as a stable and effective microbial consortium with a high potential for bioremediation of the dioxane-impacted sites and contaminated industrial wastewater.

show abstract

“…While Pseudomonas sp. have been previously reported as members of DNT-degrading consortia [3,4,24,36], several Burkholderia species have been shown to be capable of degrading DNT on their own and also as part of a consortia [4,13,37,38]. In fact, Burkholderia were the first isolated microorganisms capable of DNT degradation [39].…”

Section: Bacterial Community Diversitymentioning

confidence: 99%

The Impacts of Different Biological Treatments on the Transformation of Explosives Waste Contaminated Sludge

et al. 2021

View full text Add to dashboard Cite

The dinitrotoluene isomers 2,4 and 2,6-dinitrotoluene (DNT) represent highly toxic, mutagenic, and carcinogenic compounds used in explosive manufacturing and in commercial production of polyurethane foam. Bioremediation, the use of microbes to degrade residual DNT in industry wastewaters, represents a promising, low cost and environmentally friendly alternative technology to landfilling. In the present study, the effect of different bioremediation strategies on the degradation of DNT in a microcosm-based study was evaluated. Biostimulation of the indigenous microbial community with sulphur phosphate (2.3 g/kg sludge) enhanced DNT transformation (82% transformation, from 300 g/L at Day 0 to 55 g/L in week 6) compared to natural attenuation over the same period at 25 °C. The indigenous microbial activity was found to be capable of transforming the contaminant, with around 70% transformation of DNT occurring over the microcosm study. 16S rDNA sequence analysis revealed that while the original bacterial community was dominated by Gammaproteobacteria (30%), the addition of sulphur phosphate significantly increased the abundance of Betaproteobacteria by the end of the biostimulation treatment, with the bacterial community dominated by Burkholderia (46%) followed by Rhodanobacter, Acidovorax and Pseudomonas. In summary, the results suggest biostimulation as a treatment choice for the remediation of dinitrotoluenes and explosives waste.

show abstract

Untitled

Cited by 56 publications

References 15 publications

Aerobic Transformation of 2,4-Dinitrotoluene by Escherichia coli and Its Implications for the Detection of Trace Explosives

Aerobic Transformation of 2,4-Dinitrotoluene by Escherichia coli and Its Implications for the Detection of Trace Explosives

Enrichment and Analysis of Stable 1,4-dioxane-Degrading Microbial Consortia Consisting of Novel Dioxane-Degraders

The Impacts of Different Biological Treatments on the Transformation of Explosives Waste Contaminated Sludge

Contact Info

Product

Resources

About