742 Identification and characterization of the IX allergens of Kentucky Bluegrass (KBG) pollen

Olsen, Egil; Hill, Robert D.; Kisil, F.T.; Sehon, A.H.; Mohapatra, Shyam S.

doi:10.1016/0091-6749(91)92024-u

Cited by 15 publications

(23 citation statements)

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“…At least forty grass species are reported to be allergenic, and antigenic and allergenic cross-reactivity and high homology has been found between the group 5 allergens of many species (Klysner et al 1992, Matthiesen & Lowenstein 1991a, Olsen & al. 1991, Ong et al 1996.…”

Section: Discussionmentioning

confidence: 99%

Allergenicity of grass pollen in settled dust in rural and urban homes in Finland

Yli‐Panula

1997

Grana

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Section: Discussionmentioning

confidence: 99%

Allergenicity of grass pollen in settled dust in rural and urban homes in Finland

Yli‐Panula

1997

Grana

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“…Clearly, such crude natural protein extracts cannot be used for a specific diag nosis of allergy against a particular allergen. In addition, natural grass pollen protein extracts used for RAST and skin prick tests contain a broad variety of allergens (group I [20], group V [9], group II/group III [20,22], group IV [21] and group IX [12] allergens) that represent different IgE-binding identities and can even be further distin guished into different isoforms [14]. Despite this puzzling variety of different grass pollen allergens, we tried to es tablish a diagnostic model of grass pollen allergy based on a reasonable number of well-defined recombinant aller gens.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast to tree pollen allergy, grass pollen-allergic patients show IgE-binding to a large panel of different groups of grass pollen allergens. Two-dimensional electrophoresis and DNA sequence analysis revealed that even within one group of grass pollen allergens isoforms with different isoelectric points can be identified [14], These data create a very complex and puzzling picture regarding the number and diversity of grass pollen allergens. In order to characterize a defined and limited panel of grass pollen allergens for diagnostic and therapeutic pur poses, we chose timothy grass (Phleum pratense) as a model.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Grass Pollen Allergy with Recombinant Timothy Grass <i>(Phleum pratense)</i> Pollen Allergens

Valenta¹,

Vrtala²,

Ebner³

et al. 1992

Int Arch Allergy Immunol

137

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In order to establish a test system for grass pollen allergy based on the use of recombinant allergens we chose timothy grass (Phleum pratense), a widely spread grass, as a model. From a lambda gtll cDNA expression library that we had constructed from pollen RNA of timothy grass (P. pratense), we had obtained with serum IgE from a grass pollen-allergic individual 60 IgE-binding clones. By differential testing with sera from different grass pollen-allergic patients, we selected three distinct clones encoding Phl p I (group I), Phl p V (group V) and profilin from timothy grass, which when used together allowed the diagnosis of grass pollen allergy in 97 out of 98 tested grass pollen-allergic patients employing a simple plaque lift technique. This recombinant test based on plaque lifts containing allergen-β-galactosidase fusion proteins was compared with IgE immunoblots using crude pollen protein extracts from timothy grass. Both methods were in good agreement with RAST scores and clinical data, and proofed to be useful for the diagnosis of grass pollen allergy. Our results further indicate that a limited panel of only two recombinant grass pollen allergens, Phl p I and Phl p V, together with the plant panallergen profilin could be sufficient for the diagnosis and possibly immunotherapy of grass pollen allergy.

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“…It is evident from electrophoretic separation that the preparations being obtainable commercially are purified and therefore contain a lower quantity of proteins than raw pollen extract. However, proteins of certain molecular weight (84 and 55,6 kDa) occur in all observed pollens and they present the main allergens apparently 20,21,22 , i.e. allergens with which at least 50 per cent of allergic patients specific react.…”

Section: Discussionmentioning

confidence: 99%

Influence of Bacterial Immunomodulators on the Induction of Specific Igg With Patients During a Peroral Hyposensitization Therapy

Hradilová¹

2001

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Peroral administration of allergen during hyposensitization therapy with allergic patients is in comparison with an administration by injection of a wilder inductor of specific IgG antibodies. The peroral administration of bacterial immunomodulators increases IgG formation significantly. The described experiment examines the influence of bacterial immunomodulator Olimunostim on the level of specific antibodies during peroral hyposensitization therapy with allergic patients.

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742 Identification and characterization of the IX allergens of Kentucky Bluegrass (KBG) pollen

Cited by 15 publications

References 0 publications

Allergenicity of grass pollen in settled dust in rural and urban homes in Finland

Allergenicity of grass pollen in settled dust in rural and urban homes in Finland

Diagnosis of Grass Pollen Allergy with Recombinant Timothy Grass <i>(Phleum pratense)</i> Pollen Allergens

Influence of Bacterial Immunomodulators on the Induction of Specific Igg With Patients During a Peroral Hyposensitization Therapy

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