[99mTc]Tricine: a Useful Precursor Complex for the Radiolabeling of Hydrazinonicotinate Protein Conjugates

Abstract

The stannous reduction of [99mTc]pertechnetate in the presence of tricine results in the formation of the new labeling precursor complex [[99m-Tc]tricine. This complex has improved efficacy for the 99mTc labeling of hydrazinonicotinate-modified IgG compared to [99mTc]glucoheptonate. FAB mass spectral analysis of the product formed by the reaction of [TcOCl4](-1) with tricine indicates the formation of [TcO(tricine x 2H)2](-1).

Platelet binding and biodistribution of [99mTc]rBitistatin in animal species and humans

“…The product, which contained approximately two HYNIC groups per molecule, was purified by reversed-phase HPLC and was freeze-dried in 50 ug aliquots in amber vials and stored at −70°C. Labeling with 99m Tc was accomplished using a tricine intermediate (13) by adding up to 1 mL [ 99m Tc] pertechnetate (up to 3700 MBq/mL) to a freeze-dried vial containing 54 mg tricine and 75 ug stannous chloride dihydrate, pH adjusted to 7.3. After 2 minutes at room temperature, a portion (up to 1850 MBq) of the 99m Tc-tricine was drawn up and mixed with 50 µg HYNIC-rBitistatin.…”

Imaging and tissue biodistribution of 99mTc-labeled adenovirus knob (serotype 5)

“…5 The modified proteins were radiolabeled with 99m Tc using tricine as the transfer ligand and purified from nonbound 99m Tc by G-25 size exclusion chromatography. 28 Protein concentrations of the collected fractions were determined by the method of Lowry. 29 The protein-bound 99m Tc was evaluated by thin-layer chromatography (TLC).…”

Radioimmunodetection of Atherosclerotic Lesions Focusing on the Accumulation Mechanism of Immunoglobulin G

“…In brief, to HYNIC-N-hydroxysuccinimide (8.25 μg) in N,N-dimethylformamide (8.25 μl), TSP4-mAb or NC-mAb solution in 0.16 M borate buffer (pH 8.0) (250 μl, 2 mg/ml) was added, and the Tc-NC-mAb preparation mixture was incubated at room temperature for 2 h. The mixture was purified by size-exclusion filtration using a diafiltration membrane (Amicon Ultra 4 [cutoff molecular weight, 30,000]; Millipore Co., Billerica, MA). 99m Tc-(tricine) 2 (740 MBq/ml, 300 μl) was prepared by the method of Larsen et al [9]. It was added to the purified solution of HYNIC-TSP4-mAb or NC-mAb solution in 10 mM citrate buffer (pH 5.2) (30 μl, 1 mg/ml), and the mixture was incubated at room temperature for 1 h. The mixture was then purified on a Sephadex G-25 column (PD-10, GE Healthcare, Buckinghamshire, UK) equilibrated with 0.1 M PBS (pH7.4) to obtain 99m Tc-TSP4-mAb or 99m Tc-NC-mAb.…”