9G DNAChip: a platform for the efficient detection of proteins

Song, Ki-Bong; Nimse, Satish Balasaheb; Kim, Junbae; Kim, Jung-Hoon; Ta, Van-Thao; Nguyen, Van-Thuan; Kim, Taisun

doi:10.1039/c1cc12721g

Cited by 18 publications

(16 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The multiply infected samples were found to have only two genotypes in the HPV 9G DNA chip test, which is quite unusual, but it is due to the primer set used in this study, which is well known to express two major genotypes in multiply infected samples (18). The HPV 9G DNA chip test has proven that the preliminary findings of cervical cytology of HPV-infected samples may lead to the false detection of HPV.…”

Section: Discussionmentioning

confidence: 77%

See 1 more Smart Citation

HPV 9G DNA Chip: 100% Clinical Sensitivity and Specificity

Song

Nimse

et al. 2012

J Clin Microbiol

Self Cite

View full text Add to dashboard Cite

We describe a novel HPV 9G DNA chip test for the accurate and reliable genotyping of human papillomavirus (HPV). The HPV 9G DNA chip test established its efficiency in terms of a signal-to-background ratio (SBR) of 200, which is 50 times superior to commercial HPV DNA chips, and 100% target-specific hybridization at 25°C. We compared the genotyping results for the 439 clinical samples by the HPV 9G DNA chip test with the sequencing results for the MY11/GP6؉ (M2) primer set-mediated PCR products. The discrimination of HPV genotypes in the 151 HPV-positive clinical samples by the HPV 9G DNA chip test were 100% identical with the sequencing analysis. The clinical sensitivities of HPV genotyping by the HPV 9G DNA chip test and a commercial HPV DNA chip test were 100% and 88%, respectively. However, the clinical specificities of HPV genotyping by the HPV 9G DNA chip test and the commercial HPV DNA chip test were 100% and 94%, respectively. The 100% clinical sensitivity and specificity of the HPV 9G DNA chip test make it a promising diagnostic tool for HPV genotyping.

show abstract

Section: Discussionmentioning

confidence: 77%

“…Recently reported 9G (GGGGGGGGG) DNA chips based on 9G DNA chip technology show high SBR (PMT gain ϭ 48%) and 100% target-specific hybridization, with more than 90% hybridization efficiency at 25°C in 30 min (17,18 …”

mentioning

confidence: 99%

HPV 9G DNA Chip: 100% Clinical Sensitivity and Specificity

Song

Nimse

et al. 2012

J Clin Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…According to the recently reported DNA-Guided Detection (DAGON) method [ 116 ] based on 9G technology, antigens with concentrations in the 1 pg/mL to 10 pg/mL range can be easily differentiated. The major difference between the DAGON and other methods is that the antigen–antibody biomolecular complexes are allowed to form in the solution.…”

Section: Applications Of Microarray Technologymentioning

confidence: 99%

Immobilization Techniques for Microarray: Challenges and Applications

Nimse

Song²,

Sonawane

et al. 2014

Sensors

Self Cite

154

114

View full text Add to dashboard Cite

The highly programmable positioning of molecules (biomolecules, nanoparticles, nanobeads, nanocomposites materials) on surfaces has potential applications in the fields of biosensors, biomolecular electronics, and nanodevices. However, the conventional techniques including self-assembled monolayers fail to position the molecules on the nanometer scale to produce highly organized monolayers on the surface. The present article elaborates different techniques for the immobilization of the biomolecules on the surface to produce microarrays and their diagnostic applications. The advantages and the drawbacks of various methods are compared. This article also sheds light on the applications of the different technologies for the detection and discrimination of viral/bacterial genotypes and the detection of the biomarkers. A brief survey with 115 references covering the last 10 years on the biological applications of microarrays in various fields is also provided.

show abstract

“…[16][17][18][19] The amount of CIC in a lung cancer sample was determined by using the in-house sandwich immunoassay based on a 9G DNAChip 19 and a DAGON method. 20 The lung cancer sample was found to contain 5.0 ng mL À1 CIC (Fig. S1, ESI †) and was used as a standard sample for the development of CIC and the detection method.…”

mentioning

confidence: 99%