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Maksimenko, A. V.; Tischenko, Elena G.; Golubykh, V. L.

doi:10.1023/a:1007815418082

Cited by 13 publications

(10 citation statements)

References 37 publications

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“…The optimum SOD/CAT ratio in the SOD-CS-CAT conjugate corresponded to 25 -50 U (SOD) and 55 -110 U (CAT) per rat. Such small values of the effective doses indicate that, probably, a self-healing antioxidant protective layer is formed on the cell surface [52,57]. These results show good prospects in the further study of the SOD-CS-CAT conjugate, which exceeds in antithrombotic activity all other combinations (Fig.…”

Section: Using Sod and Cat Combinations For Antioxidant Protection Ofmentioning

confidence: 92%

“…Taking into account that aging and, the more so, atherosclerotic damage, lead to an increase in the content of CS in the human glycocalyx [56], this glycosaminoglycan is important for imparting, to SOD-1, affinity to the zones of risk and the sites of damage on the vas-cular wall. The covalent conjugation of Cu/ZN-SOD to CS made possible a comparative study of the efficacy of antithrombotic action for the SOD-CS complex, the native enzyme (free of CS), and their simple mixture (SOD + CS) in rats with a model of arterial thrombosis induced by iron chloride treatment of blood vessels [57]. The efficacy of the antithrombotic action was evaluated (relative to untreated control) from the time of arterial occlusion and the weight of the thrombus.…”

Section: Approaches To Pharmacological Modification Of Cu/zn-sod As Ementioning

confidence: 99%

“…The covalent SOD-CS conjugate exhibited the maximum antithrombotic effect. It can be suggested that the increase in the antioxidant activity of SOD-CS as compared to that of SOD is related to increased affinity of the former to the vessel wall as a result of this modification [57]. This conjugate offers a promising means of protecting vascular endothelium against the detrimental action of ROS.…”

Section: Approaches To Pharmacological Modification Of Cu/zn-sod As Ementioning

confidence: 99%

“…However, this approach gave contradictory results in the experiments with native forms of enzymes, which was related in particular to their inability to penetrate into the intracellular space upon intravenous infusion [46]. The modified derivatives SOD-CS and CAT-CS exhibit reliably higher antithrombotic activity than the separate components or simple mixtures in comparable doses [57,76]. The combination of SOD-CS and CAT-CS did not offer advantages in comparison to the separate components [69], which was probably related to the difference in the intravessel surface distribution of these derivatives in the bloodstream.…”

Section: Using Sod and Cat Combinations For Antioxidant Protection Ofmentioning

confidence: 99%

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Extracellular oxidative damage of vascular walls and their protection using antioxidant enzymes

Maksimenko

2007

Pharm Chem J

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Reactive oxygen species damage blood vessel walls in both initial and subsequent stages of vascular pathology. Oxidative stress contributes to the atherogenic modification of low-density lipoproteins, the development of lipid streaks on the luminal surface, the disturbance of the glycocalyx, and the ulceration and rupture of atherosclerotic plaques. Such a broad spectrum of damaging activity implies the need for both intra-and extracellular protection of vascular walls, which can be realized by the combined use of gene therapy and exogenous biological antioxidants. Recent in vivo studies have shown that modified superoxide dismutase (SOD) and catalase as well as extracellular SOD (an enzyme that employs the specificity of glycosaminoglycan -protein interactions) can be effectively used as antioxidants. Covalent binding of SOD to catalase via the vascular wall glycosaminoglycan (chondroitin sulfate) leads to the formation of a bienzyme conjugate, which exhibits more pronounced antithrombotic activity in vivo in comparison to several other SOD and catalase derivatives. These results point to the importance of the attachment of bioantioxidants to the vascular wall and the conjugation of SOD and catalase activities on this surface. Extracellular bioantioxidants have good prospects for the development of highly effective protectors of vascular walls against oxidative damage in the framework of complex therapy of cardiovascular injury. 235 0091-150X/07/4105-0235

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Section: Using Sod and Cat Combinations For Antioxidant Protection Ofmentioning

confidence: 92%

Section: Approaches To Pharmacological Modification Of Cu/zn-sod As Ementioning

confidence: 99%

Section: Approaches To Pharmacological Modification Of Cu/zn-sod As Ementioning

confidence: 99%

Section: Using Sod and Cat Combinations For Antioxidant Protection Ofmentioning

confidence: 99%

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Extracellular oxidative damage of vascular walls and their protection using antioxidant enzymes

Maksimenko

2007

Pharm Chem J

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“…Thrombolytic compositions based on the third-generation PA (chemically or biologically modified PA derivatives) open new prospectives in clinical thrombolysis [56,57]. Which way or which composition is more preferable for treatment of acute thrombosis it is difficult to answer now.…”

Section: Total Fibrinolytic Effectmentioning

confidence: 99%

Macromolecular Ensembles of Internal and External Fibrinolysis: the Resources for Enhancement of Thrombolysis Efficacy

Maksimenko

Tischenko²

2006

CMC

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The results of the search for new plasminogen activators for thrombolytic therapy have been reviewed with analysis of slowdown in this process. The reserves of increasing the effectiveness of thrombolysis are considered and the mechanisms underlying the interactions between plasminogen and its activators with fibrin are described. The domain composition of the fibrinolytic agents and the functional role of their structural elements in fibrinolytic interactions are discussed. The action of fibrin-specific and fibrin-nonspecific plasminogen activators in fibrinolysis has been evaluated. The necessity of the investigation of the regularities of internal and external fibrinolysis has been substantiated. The internal fibrinolysis became the resource for enhancement of thrombolysis efficacy. The approaches to the use of internal fibrinolysis to increase the effectiveness of enzyme therapy (monotherapy with plasminogen or new-made plasminogen activator as well as polytherapy with combination of different plasminogen activators /thrombolysis trigger plus third-generation plasminogen activator of prolonged action/) have been outlined and the relationship between this research and the current tendencies in the improvement of clinical thrombolysis (dose reduction, adjacent therapy, etc.) has been discussed.

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Extracellular protection of the vascular wall with the covalently connected antioxidant enzymes against oxidative damage

Maksimenko

Vavaev

Tischenko

2008

Journal of Biotechnology

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Untitled

Cited by 13 publications

References 37 publications

Extracellular oxidative damage of vascular walls and their protection using antioxidant enzymes

Extracellular oxidative damage of vascular walls and their protection using antioxidant enzymes

Macromolecular Ensembles of Internal and External Fibrinolysis: the Resources for Enhancement of Thrombolysis Efficacy

Extracellular protection of the vascular wall with the covalently connected antioxidant enzymes against oxidative damage

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