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Kang, Hee Wan; Cho, Yong Gu; Yoon, Ung Han; Eun, Moo Young

doi:10.1023/a:1007418606098

Cited by 79 publications

(14 citation statements)

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“…For molecular analyses, the genomic DNA from a single dry seed was isolated by modified method of [14], total eight RAPD and seven SSR markers were utilized, out of these five RAPD and five SSR markers indicated polymorphism shown in (Tables 1 and 2). The PCR for RAPD was conducted for 40 cycles with the initial denaturation temperature at 94˚C for 2 min, annealing at 36˚C for one minute and final extension temperature for each primer was fixed at 72˚C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Diversity analysis of chickpea (<i>Cicer arietinum</i> L.) germplasm and its implications for conservation and crop breeding

Ahmad¹,

Mumtaz²,

Nisar³

et al. 2012

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The exploration of genetically variable accessions is the key source of germplasm conservation and potential breeding material for the future. The more diverse group of cultivars provides an ample opportunity to breeders for releasing new and superior varieties, considering their quality traits for direct commercial utilization. In this study, we assessed the genetic diversity of Cicer arietinum 70 accessions from Pakistan and USA using morphological traits, seed protein and molecular markers. Based on four morphological traits, the average coefficient of variation was calculated as 56.8% with significant correlation among yield traits. The analysis revealed that the accessions 1898, 2819, 3022, 3037, 3040, 3043, 3054, 3059 and 3063 were best in performance with a total of 12% environmental error. The cluster analysis based on protein data revealed 50% genetic diversity among accessions. The clustering pattern did not show any grouping that could be attributed to either the geographic distribution or the field performance. For molecular characterization of germplasm 5 PCR based RAPD primers, OPA4, OPA9, OPG13, UBC181 and UBC733b used were found to be polymorphic with 37% genetic diversity among local and exotic accessions. Whereas, 3 SSR primers viz., CaSTMS2, CaSTMS15 and CaSTMS21 scored the genetic variability up to 55% by cluster analysis through UPGMA percent disagreement. The primers, TA72 and TA130 were linked with yield related traits, indicated highest dissimilarity index value (0.69) and notable variation in the identified promising lines. The Morphometric, Biochemical and Molecular markers reported here, are helpfulto assess the extent of genetic diversity among Chickpea accessions and can be used to identify the unreported cultivars with desirable quantitative traits for improving Chickpea yield in Pakistan. Based on the study, the accessions 3043 and 3054 have been recommended to the breeders for their future use in multiplication and screening against various diseases.

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Section: Methodsmentioning

confidence: 99%

Diversity analysis of chickpea (<i>Cicer arietinum</i> L.) germplasm and its implications for conservation and crop breeding

Ahmad¹,

Mumtaz²,

Nisar³

et al. 2012

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“…After removing the seed coat, each seed was cut into pieces and incubated at 37°C in DNA extraction buffer (200 mM Tris-HCl pH 8.0, 200 mM NaCl, 25 mM ethylenediaminetetraacetic acid, and 0.5% sodium dodecyl sulfate) containing 0.012% proteinase K. The soaked seed was ground and then extracted with 2% CTAB buffer and phenol-choloroform. Finally, RNA was eliminated by RNase treatment as described by Kang et al (1998). The extracted DNA was further purified using the phenol-chloroform method and quantified by both visual quantification (agarose gel) and UV spectrophotometry.…”

Section: Methodsmentioning

confidence: 99%

Molecular Marker Analysis of Differentially Aged Seeds of Soybean and Safflower

et al. 2009

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Storage of seeds for extended periods causes a number of degradative changes related to the aging process such as decreased seedling vigor and reduced germination. In this study, molecular markers were used to study the aging process in seeds of two different plants species. Seeds of three differentially aged seed groups, including control (un-aged), naturally aged, and accelerated aging, from soybean (Glycine max) and safflower (Carthamus tinctorius) were evaluated for genetic variability using random amplification of polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and simple sequence repeat (SSR) markers. For both plant species, naturally aged and accelerated aged groups clustered together with RAPD markers, whereas control and naturally aged seeds showed similarity in both AFLP and SSR profiles. Based on these findings, it can be concluded that observed changes in DNA profiles of seeds from different aged groups did not contribute to accumulation of genetic variations of the same magnitude. Therefore, seed of similar viability must be selected for molecular marker analysis for plant variety protection, among other comparative studies.

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“…Genomic DNA was extracted from a single dried wheat seed using the method described by Kang et al (1998). Primers (PPO05) were designed using the Primer Premier 5.0 software based on the sequence information of PPO EST AY515506 sequence, and used to screen the cultivars with low and high PPO activities.…”

Section: Primer Design and Sts Analysismentioning

confidence: 99%

Gene markers for grain polyphenol oxidase activity in common wheat

Wang

et al. 2008

Mol Breeding

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Polyphenol oxidase (PPO) in grain is regarded as a major factor resulting in time-dependent darkening of wheat end products, particularly for Asian noodles and steamed bread. Breeding wheat cultivars with low PPO activity using efficient and reliable markers is one of the best ways to reduce the undesirable darkening. In the present study, we developed a gene-specific marker (PPO05) for low PPO activity from the sequence AY515506. This marker detected double PCR fragments (\750 and [750 bp) in the cultivars with low PPO activity and a single PCR fragment (\750 bp) in the cultivars with high PPO activity. Screening of this marker on 235 Chinese wheat micro-core collections showed that the double fragments were present in 113 genotypes and the single fragments in the remaining 122 genotypes. Statistic analysis revealed that the cultivars with the double fragments had significantly lower mean PPO activity than those with single fragments. Through sequence analysis and blast search in NCBI, we found that the cultivars with the double fragments contained the PPO-2Ab allele, while the cultivars with the single fragments contained the PPO-2Aa allele. The PPO-2Ab and PPO-2Da alleles were associated with the low grain PPO activity and the PPO-2Aa and PPO-2Db alleles associated with the high PPO activity. The genotypes carrying both PPO2Ab and PPO-2Da showed the lowest PPO activity, while the genotypes carrying both PPO-2Aa and PPO-2Db showed the highest PPO activity. Comparison of PPO05 and STS01 with the STS markers PPO18 and PPO29 showed that the larger and small fragments of PPO05 were equivalent to the 876-and 685-bp fragments of PPO18, respectively, and that STS01 was the complementary marker of PPO29. Thus, the STS markers PPO05 and STS01 along with PPO18 and PPO29 are the efficient and reliable markers for the evaluation of PPO activity and can be used in wheat breeding programs to improve the quality of noodles and other end products.

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Cited by 79 publications

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Diversity analysis of chickpea (<i>Cicer arietinum</i> L.) germplasm and its implications for conservation and crop breeding

Diversity analysis of chickpea (<i>Cicer arietinum</i> L.) germplasm and its implications for conservation and crop breeding

Molecular Marker Analysis of Differentially Aged Seeds of Soybean and Safflower

Gene markers for grain polyphenol oxidase activity in common wheat

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Cited by 79 publications

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Diversity analysis of chickpea (&lt;i&gt;Cicer arietinum&lt;/i&gt; L.) germplasm and its implications for conservation and crop breeding

Diversity analysis of chickpea (&lt;i&gt;Cicer arietinum&lt;/i&gt; L.) germplasm and its implications for conservation and crop breeding

Molecular Marker Analysis of Differentially Aged Seeds of Soybean and Safflower

Gene markers for grain polyphenol oxidase activity in common wheat

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Diversity analysis of chickpea (<i>Cicer arietinum</i> L.) germplasm and its implications for conservation and crop breeding

Diversity analysis of chickpea (<i>Cicer arietinum</i> L.) germplasm and its implications for conservation and crop breeding