A (1→4)-β-mannan-specific monoclonal antibody and its use in the immunocytochemical location of galactomannans

Pettolino, Filomena; Hoogenraad, Nicholas J.; Ferguson, C.; Bacic, Antony; Johnson, Elizabeth; Stone, Bruce

doi:10.1007/s004250100606

Cited by 60 publications

(36 citation statements)

References 32 publications

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“…In the case of the latter polysaccharides, an immunocytochemical approach to their detection with polysaccharide-specific antibodies has provided definitive proof of their synthesis in the Golgi (pectin and xyloglucan [Zhang and Staehelin, 1992]; mannan [Pettolino et al, 2001;Wilson et al, 2006]; AX [Philippe et al, 2006;Wilson et al, 2006]). This same approach has also been used to locate the site of MLG biosynthesis but has drawn contrasting conclusions in different Poaceae species.…”

Section: Discussionmentioning

confidence: 99%

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Determining the Subcellular Location of Synthesis and Assembly of the Cell Wall Polysaccharide (1,3; 1,4)-β-d-Glucan in Grasses

Wilson

Lampugnani

et al. 2015

The Plant Cell

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The current dogma for cell wall polysaccharide biosynthesis is that cellulose (and callose) is synthesized at the plasma membrane (PM), whereas matrix phase polysaccharides are assembled in the Golgi apparatus. We provide evidence that (1,3;1,4)-b-D-glucan (mixed-linkage glucan [MLG]) does not conform to this paradigm. We show in various grass (Poaceae) species that MLG-specific antibody labeling is present in the wall but absent over Golgi, suggesting it is assembled at the PM. Antibodies to the MLG synthases, cellulose synthase-like F6 (CSLF6) and CSLH1, located CSLF6 to the endoplasmic reticulum, Golgi, secretory vesicles, and the PM and CSLH1 to the same locations apart from the PM. This pattern was recreated upon expression of VENUS-tagged barley (Hordeum vulgare) CSLF6 and CSLH1 in Nicotiana benthamiana leaves and, consistent with our biochemical analyses of native grass tissues, shown to be catalytically active with CSLF6 and CSLH1 in PM-enriched and PM-depleted membrane fractions, respectively. These data support a PM location for the synthesis of MLG by CSLF6, the predominant enzymatically active isoform. A model is proposed to guide future experimental approaches to dissect the molecular mechanism(s) of MLG assembly.

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Section: Discussionmentioning

confidence: 99%

“…Antibodies to MLG (Meikle et al, 1994), heteromannan (Pettolino et al, 2001), and (1,3)-b-D-glucan (Meikle et al, 1991) were from Biosupplies. A gold-conjugated goat anti-rabbit IgG secondary antibody (10 or 18 nm; Jackson ImmunoResearch) was used at 1:20 for visualization.…”

Section: Antibodiesmentioning

confidence: 99%

Determining the Subcellular Location of Synthesis and Assembly of the Cell Wall Polysaccharide (1,3; 1,4)-β-d-Glucan in Grasses

Wilson

Lampugnani

et al. 2015

The Plant Cell

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“…Grain samples from 10 to 28 DAP were prepared for microscopy and changes to the endosperm were monitored by taking transverse sections of the developing grains. The distribution and relative abundance of polysaccharides in the differentiating grain were detected on sections using monoclonal antibodies raised against specific polysaccharide epitopes including (1→3)-b-D-glucan (Meikle et al, 1991), xyloglucan LM15 (Marcus et al, 2008), (1→3,1→4)-b-D-glucan (Meikle et al, 1994), hetero-(1→4)-b-Dmannan (Pettolino et al, 2001), and arabino-(1-4)-b-D-xylan LM11 (McCartney et al, 2005). Sections for light microscopy (1 mm) were either stained in toluidine blue to highlight tissue architecture or processed for immunogold labeling.…”

Section: Immunolabeling For Light and Emmentioning

confidence: 99%

Pattern of Deposition of Cell Wall Polysaccharides and Transcript Abundance of Related Cell Wall Synthesis Genes during Differentiation in Barley Endosperm

Wilson

Burton²,

Collins³

et al. 2012

Plant Physiology

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Immunolabeling, combined with chemical analyses and transcript profiling, have provided a comprehensive temporal and spatial picture of the deposition and modification of cell wall polysaccharides during barley (Hordeum vulgare) grain development, from endosperm cellularization at 3 d after pollination (DAP) through differentiation to the mature grain at 38 DAP. (1→3)-β-d-Glucan appears transiently during cellularization but reappears in patches in the subaleurone cell walls around 20 DAP. (1→3, 1→4)-β-Glucan, the most abundant polysaccharide of the mature barley grain, accumulates throughout development. Arabino-(1-4)-β-d-xylan is deposited significantly earlier than we previously reported. This was attributable to the initial deposition of the polysaccharide in a highly substituted form that was not recognized by antibodies commonly used to detect arabino-(1-4)-β-d-xylans in sections of plant material. The epitopes needed for antibody recognition were exposed by pretreatment of sections with α-l-arabinofuranosidase; this procedure showed that arabino-(1-4)-β-d-xylans were deposited as early as 5 DAP and highlighted their changing structures during endosperm development. By 28 DAP labeling of hetero-(1→4)-β-d-mannan is observed in the walls of the starchy endosperm but not in the aleurone walls. Although absent in mature endosperm cell walls we now show that xyloglucan is present transiently from 3 until about 6 DAP and disappears by 8 DAP. Quantitative reverse transcription-polymerase chain reaction of transcripts for GLUCAN SYNTHASE-LIKE, Cellulose Synthase, and CELLULOSE SYNTHASE-LIKE genes were consistent with the patterns of polysaccharide deposition. Transcript profiling of some members from the Carbohydrate-Active Enzymes database glycosyl transferase families GT61, GT47, and GT43, previously implicated in arabino-(1-4)-β-d-xylan biosynthesis, confirms their presence during grain development.

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“…In our previous work, LM10 and LM11 antibodies showed some differences in AGX localization (Kim et al, 2010c). Some sections were also incubated with BGM C6 monoclonal antibody specific to (1→4)-β-linked mannopyranosyl residues (Pettolino et al, 2001). Thereafter, grids were incubated with goat anti-rat (LM10 and LM11) or anti-mouse (BGM C6) secondary antibody labeled with 10-nm colloidal gold particles for 1 h at room temperature.…”

Section: Immunogold Labelingmentioning

confidence: 99%

Distribution of Hemicelluloses in Warts and the Warty Layer in Normal and Compression Wood Tracheids of Cryptomeria Japonica

Kim¹,

Awano²,

Yoshinaga³

et al. 2011

Journal of the Korean Wood Science and Technology

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The distribution of arabino-4-O-methylglucuronoxylans (AGXs) and O-acetyl-galactoglucomannans (GGMs) in warts and the warty layer of tracheids in normal wood (NW) and compression wood (CW) of Cryptomeria japonica was investigated. Under field emission scanning electron microscope (FE-SEM) observation, warts and the warty layer of delignified NW and CW tracheids were degraded by xylanase treatment, indicating that warts and the warty layer contain high amounts of AGXs. However, the effect of xylanase was not observed in NW and CW tracheids before delignification, suggesting that AGXs in warts and the warty layer may be encrusted with lignin. After β-mannanase treatment, no noticeable changes were observed in warts and the warty layer of NW tracheids, indicating that warts and the warty layer contain either no or very few GGMs. Similar results to FE-SEM observations were also observed with immunogold labeling. AGX labeling was observed in warts and the warty layer of NW and CW tracheids, while GGM labeling was not detected. NW tracheids showed a much stronger density of AGX labeling than did CW tracheids in warts and the warty layer, indicating differences in the chemical compositions of warts and the warty layer between NW and CW tracheids.

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A (1→4)-β-mannan-specific monoclonal antibody and its use in the immunocytochemical location of galactomannans

Cited by 60 publications

References 32 publications

Determining the Subcellular Location of Synthesis and Assembly of the Cell Wall Polysaccharide (1,3; 1,4)-β-d-Glucan in Grasses

Determining the Subcellular Location of Synthesis and Assembly of the Cell Wall Polysaccharide (1,3; 1,4)-β-d-Glucan in Grasses

Pattern of Deposition of Cell Wall Polysaccharides and Transcript Abundance of Related Cell Wall Synthesis Genes during Differentiation in Barley Endosperm

Distribution of Hemicelluloses in Warts and the Warty Layer in Normal and Compression Wood Tracheids of Cryptomeria Japonica

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