A 26-base-pair repetitive sequence specific for Neisseria gonorrhoeae and Neisseria meningitidis genomic DNA

Correia, Frederick F.; Inouye, Sumiko; Inouye, Masayori

doi:10.1128/jb.167.3.1009-1015.1986

Cited by 85 publications

(63 citation statements)

References 21 publications

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“…The bcr1 element is similar in length and distribution to several DNA repeats identified previously in other bacteria, most prominently the Correia elements found in Neisseria species (4,6,7,22). However, the composition of the elements is different; while the Correia elements have a fixed length, a highly modular substructure, and 25-bp inverted repeats followed by TA dinucleotide direct repeats flanking the ends (4,22), bcr1 exhibits a mosaic substructure (Fig.…”

Section: Discussionmentioning

confidence: 77%

The bcr1 DNA Repeat Element Is Specific to the Bacillus cereus Group and Exhibits Mobile Element Characteristics

et al. 2004

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Bacillus cereus strains ATCC 10987 and ATCC 14579 harbor a ϳ155-bp repeated element, bcr1, which is conserved in B. cereus, B. anthracis, B. thuringiensis, and B. mycoides but not in B. subtilis and B. licheniformis. In this study, we show by Southern blot hybridizations that bcr1 is present in all 54 B. cereus group strains tested but absent in 11 Bacillus strains outside the group, suggesting that bcr1 may be specific and ubiquitous to the B. cereus group. By comparative analysis of the complete genome sequences of B. cereus ATCC 10987, B. cereus ATCC 14579, and B. anthracis Ames, we show that bcr1 is exclusively present in the chromosome but absent from large plasmids carried by these strains and that the numbers of full-length bcr1 repeats for these strains are 79, 54, and 12, respectively. Numerous copies of partial bcr1 elements are also present in the three genomes (91, 128, and 53, respectively). Furthermore, the genomic localization of bcr1 is not conserved between strains with respect to chromosomal position or organization of gene neighbors, as only six full-length bcr1 loci are common to at least two of the three strains. However, the intergenic sequence surrounding a specific bcr1 repeat in one of the three strains is generally strongly conserved in the other two, even in loci where bcr1 is found exclusively in one strain. This finding indicates that bcr1 either has evolved by differential deletion from a very high number of repeats in a common ancestor to the B. cereus group or is moving around the chromosome. The identification of bcr1 repeats interrupting genes in B. cereus ATCC 10987 and ATCC 14579 and the presence of a flanking TTTAT motif in each end show that bcr1 exhibits features characteristic of a mobile element.

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Section: Discussionmentioning

confidence: 77%

The bcr1 DNA Repeat Element Is Specific to the Bacillus cereus Group and Exhibits Mobile Element Characteristics

et al. 2004

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“…It is possible that the PchR homologue may play a critical role in regulating expression of TdfF in the presence of an unidentified siderophore. However, further analysis also revealed an Inouye\Correia type repeat (Correia et al, 1986(Correia et al, , 1988 between the upstream ceuE homologue and the putative ribosome-binding site of tdfF in FA1090. Similarly, another repeat was present in N. meningitidis strain Z2491 but in a different location, in the middle of the ceuE homologue.…”

Section: Discussionmentioning

confidence: 99%

Neisserial TonB-dependent outer-membrane proteins: detection, regulation and distribution of three putative candidates identified from the genome sequences The GenBank accession number for the sequence of tdfH from meningococcal strain IR1074 reported in this paper is AF227418.

et al. 2001

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Computer searches were carried out of the gonococcal and meningococcal genome databases for previously unknown members of the TonB-dependent family (Tdf) of outer-membrane receptor proteins. Seven putative noncontiguous genes were found and three of these (identified in gonococcal strain FA1090) were chosen for further study. Consensus motif analysis of the peptide sequences was consistent with the three genes encoding TonBdependent receptors. In view of the five previously characterized TonBdependent proteins of pathogenic neisseriae, the putative genes were labelled tdfF, tdfG and tdfH. TdfF had homology with the siderophore receptors FpvA of Pseudomonas aeruginosa and FhuE of Escherichia coli, whereas TdfG and TdfH had homology with the haemophore receptor HasR of Serratia marcescens. The aim of this project was to characterize these proteins and determine their expression, regulation, distribution and surface exposure. Strain surveys of iron-stressed commensal and pathogenic neisseriae revealed that TdfF is unlikely to be expressed, TdfG is expressed by gonococci only and that TdfH is expressed by both meningococci and gonococci. Expression of TdfH was unaffected by iron availability. Susceptibility of TdfH to cleavage by proteases in live gonococci was consistent with surface exposure of this protein. TdfH may function as a TonB-dependent receptor for a non-iron nutrient source. Furthermore, TdfH is worthy of future investigation as a potential meningococcal vaccine candidate as it is a highly conserved, widely distributed and surface-exposed outer-membrane protein.

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“…Of these, many are variously combined in intergenic repeat arrays plausibly involved in recombination processes frequently occurring in Neisseriae [1]. Other DNA repeats, as the so-called Correia [3] or nemis [4] sequences, are predominantly found as individual units. Nemis are miniature DNA insertion sequences (80-160 bp), which feature 26 -27 bp long terminal inverted repeats (TIRs).…”

Section: Introductionmentioning

confidence: 99%

The abundant class of nemis repeats provides RNA substrates for ribonuclease III in Neisseriae

Gregorio

Abrescia

Carlomagno

et al. 2002

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expres

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About 2% of the Neisseria meningitidis genome is made up by nemis, short DNA sequences which feature long terminal inverted repeats (TIRs). Most nemis are interspersed with single-copy DNA and are found at close distance from cellular genes. In this work, we demonstrate than RNAs spanning nemis of different length and sequence compositions are specifically cleaved at hairpins formed by nemis termini by total cellular lysates derived from both Escherichia coli and Neisseria lactamica strains. The use of cellular extracts from E. coli strains impaired in the activity of known ribonucleases let to establish that cleavage at nemis TIRs is specifically mediated by the endoribonuclease RNase III. Data set the base for the identification of all of the neisserial genes that are regulated by RNase III because of their physical association with nemis DNA. D 2002 Published by Elsevier Science B.V.

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A 26-base-pair repetitive sequence specific for Neisseria gonorrhoeae and Neisseria meningitidis genomic DNA

Cited by 85 publications

References 21 publications

The bcr1 DNA Repeat Element Is Specific to the Bacillus cereus Group and Exhibits Mobile Element Characteristics

The bcr1 DNA Repeat Element Is Specific to the Bacillus cereus Group and Exhibits Mobile Element Characteristics

Neisserial TonB-dependent outer-membrane proteins: detection, regulation and distribution of three putative candidates identified from the genome sequences The GenBank accession number for the sequence of tdfH from meningococcal strain IR1074 reported in this paper is AF227418.

The abundant class of nemis repeats provides RNA substrates for ribonuclease III in Neisseriae

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