A 31P-NMR study on the recovery of intracellular pH in LLC-PK1Cl4 cells from intracellular alkalinization

Jans, A. W. H.; Krijnen, Erik S.; Luig, Jutta; Kinne, Rolf

doi:10.1016/0167-4889(87)90223-0

Cited by 10 publications

(1 citation statement)

References 22 publications

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“…E49 cells are an endothelial cell line. The LLC-PK1 cell line, a renal epithelial cell line (Jans et al 1987) with characteristics of proximal tubular cells, was obtained from the laboratory of Professor Dr Heini Murer (Zürich, Switzerland). TALH cells were cultured in DMEM (Dulbecco's Modified Eagle's Medium), E49 cells were maintained in Ham's F-12 medium and LLC-PK1 cells were cultured in Eagle's minimal essential medium.…”

Section: Chemicals and Solutionsmentioning

confidence: 99%

BCECF in Single Cultured Cells: Inhomogeneous Distribution but Homogeneous Response

Weinlich

Theiss

Lin

et al. 1998

Journal of Experimental Biology

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Using confocal laser scanning microscopy with a dual-wavelength laser system, the behaviour of BCECF [(2',7'-bis-2-carboxyethyl)-5-(and-6)carboxyfluorescein] was investigated in a variety of cell lines. Selection of a small area for monitoring allowed discrimination between various intracellular organelles, whose identity was established by vital staining. It was found that, after loading the cells with BCECF, both the nucleus and the mitochondria showed a higher level of fluorescence than the cytoplasm. Calibration of the pH-sensitivity of these fluorescence signals using the nigericin method yielded identical curves, as did exposure of the cells to NH4Cl. These studies suggest that BCECF, despite its inhomogeneous intracellular distribution, reports the pH of only one cellular compartment, the cytosol.

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Section: Chemicals and Solutionsmentioning

confidence: 99%

BCECF in Single Cultured Cells: Inhomogeneous Distribution but Homogeneous Response

Weinlich

Theiss

Lin

et al. 1998

Journal of Experimental Biology

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Intracellular pH

Putnam

Roos

1997

Comprehensive Physiology

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The sections in this article are: Techniques pH‐Sensitive Microelectrodes pH‐Sensitive Fluorescent Indicators Nuclear Magnetic Resonance Spectroscopy Some Observations on Intracellular pH Transients Mechanisms of pH Regulation Physicochemical Buffers Membrane Transport Systems Determinants of Steady‐State pH i Some Cellular Processes Affecting or Affected by pH i Metabolism Cell‐Cell Coupling: Gap Junctions DNA Synthesis and Cell Growth Membrane Channels Cell‐Volume Regulation Mitochondrial H + Movements Cytoskeleton Endocytosis/Exocytosis Other Cellular Processes

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A perifusion loop‐gap resonator NMR probe for aerobic cell suspensions

Anderson

Markley

Arús

et al. 1993

Magnetic Resonance in Med

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NMR studies of aerobic cell metabolism require that spectral data of high sensitivity and resolution be acquired from samples maintained under suitable conditions. The loop-gap resonator 31P NMR probe described here was designed for investigations of highly aerobic-dependent renal proximal tubules in an environment similar to those of conventional incubation flasks. The inherently higher sensitivity of the loop-gap resonator design and associated circuitry made it possible to obtain adequate spectra in short periods of time with small amounts of sample. Cellular physiological properties of perifused aerobic renal proximal tubule cell suspensions in the loop-gap resonator probe were found to be similar to equivalent tissue samples incubated in conventional flasks. Furthermore, the loop-gap perifusion probe was found to provide useful 31P NMR spectra of the kidney tubule system after acquisition times as short as 3 min.

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A 31P-NMR study on the recovery of intracellular pH in LLC-PK1Cl4 cells from intracellular alkalinization

Cited by 10 publications

References 22 publications

BCECF in Single Cultured Cells: Inhomogeneous Distribution but Homogeneous Response

BCECF in Single Cultured Cells: Inhomogeneous Distribution but Homogeneous Response

Intracellular pH

A perifusion loop‐gap resonator NMR probe for aerobic cell suspensions

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