A 3D cell culture approach for studying neuroinflammation

Microglia in pediatric brain tumors: The missing link to successful immunotherapy

“…Despite progress being made, co-culture systems that accurately represent tumor-microglial communication remain scarce. 127 , 128 , 129 , 130 , 131 …”

A PrP EGFR signaling axis controls neural stem cell senescence through modulating cellular energy pathways

“…The establishment of the NSC cultures has been described previously ( 56 , 57 ). Briefly, the subventricular zone was removed from three C57BL/10SnJ WT, PrP-KO, and Tg44 GPI anchorless mice ( 58 ) and separated into a cell suspension using the Stem Cell Technologies NeuroCult Enzymatic Dissociation Kit for Adult CNS Tissue (Mouse and Rat) as per the manufacturer’s instructions.…”

Exploring the Pathophysiology of Delirium: An Overview of Biomarker Studies, Animal Models, and Tissue-Engineered Models

“…127 Collectively, these models may permit a comparative study of cellular responses during neurological disease or after environmental or chemical stressors to determine which factors are most important in neural cell survival and function and thus may also have relevance in the study of delirium. For example, investigation of the interplay between glial cells and neurons after exposure to proinflammatory stimuli in neural tissue models may reveal acute changes in signaling pathways involved during neuroinflammation, 128,129 a process that has been posited to be important in delirium onset. 58,130 One approach to study delirium using neural organoids is by pharmacological inhibition of acetylcholine secretion and uptake using atropine in a similar method used in certain animal models of delirium.…”