A 3D printable perfused hydrogel vascular model to assay ultrasound-induced permeability

Royse, Madison K.; Means, A. Kristen; Calderon, Gisele A.; Kinstlinger, Ian S.; He, Yufang; Durante, Marc R; Procopio, Adam; Veiseh, Omid; Xu, Jun

doi:10.1039/d2bm00223j

Cited by 3 publications

(7 citation statements)

References 84 publications

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“…[ 77–79 ] The main reason pushing their exploitation is the preservation of a strong angiogenic activity, leading to their proliferation on lumen‐like structures, also in vitro. [ 80 ]…”

Section: Employed Technologies and Methods For The 3d‐bioprinting Of ...mentioning

confidence: 99%

“…[77][78][79] The main reason pushing their exploitation is the preservation of a strong angiogenic activity, leading to their proliferation on lumen-like structures, also in vitro. [80] Liver sinusoids are also studded with Kupffer cells, which are hepatic macrophages. They regulate the immune response in the organ, and remove noxious compounds from the portal vein.…”

Section: Cells For Liver Bioinksmentioning

confidence: 99%

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Toward 3D‐Bioprinted Models of the Liver to Boost Drug Development

Guagliano

Volpini

Briatico‐Vangosa

et al. 2022

Macromolecular Bioscience

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The main problems in drug development are connected to enormous costs related to the paltry success rate. The current situation empowered the development of high-throughput and reliable instruments, in addition to the current golden standards, able to predict the failures in the early preclinical phase. Being hepatotoxicity responsible for the failure of 30% of clinical trials, and the 21% of withdrawal of marketed drugs, the development of complex in vitro models (CIVMs) of liver is currently one of the hottest topics in the field. Among the different fabrication techniques, 3D-bioprinting is emerging as a powerful ally for their production, allowing the manufacture of three-dimensional constructs characterized by computer-controlled and customized geometry, and inter-batches reproducibility. Thanks to these, it is possible to rapidly produce tailored cell-laden constructs, to be cultured within static and dynamic systems, thus reaching a further degree of personalization when designing in vitro models. This review highlights and prioritizes the most recent advances related to the development of CIVMs of the hepatic environment to be specifically applied to pharmaceutical research, with a special focus on 3D-bioprinting, since the liver is primarily involved in the metabolism of drugs.

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Section: Employed Technologies and Methods For The 3d‐bioprinting Of ...mentioning

confidence: 99%

Section: Cells For Liver Bioinksmentioning

confidence: 99%

Toward 3D‐Bioprinted Models of the Liver to Boost Drug Development

Guagliano

Volpini

Briatico‐Vangosa

et al. 2022

Macromolecular Bioscience

View full text Add to dashboard Cite

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“…To overcome the above drawbacks, microfabricated chips were used to study the disruption of endothelial cell–cell contract ( Figure 9 , Figure 10 , Figure 11 and Figure 12 ) by ultrasound and microbubbles [ 21 , 37 , 101 , 125 , 126 ]. A typical setup for this type of experiment is illustrated in Figure 13 .…”

Section: Ultrasound-driven Microbubbles Reversibly Open Bbbmentioning

confidence: 99%

“…Royse et al [ 126 ] reported a perfusion-based 3D printable hydrogel vascular model to assess BBB permeability and its function. Ultrasound-driven microbubbles increased the permeability of the BBB, altered cell area, and disrupted cell–cell junctions.…”

Section: Ultrasound-driven Microbubbles Reversibly Open Bbbmentioning

confidence: 99%

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Advances in BBB on Chip and Application for Studying Reversible Opening of Blood–Brain Barrier by Sonoporation

et al. 2022

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The complex structure of the blood–brain barrier (BBB), which blocks nearly all large biomolecules, hinders drug delivery to the brain and drug assessment, thus decelerating drug development. Conventional in vitro models of BBB cannot mimic some crucial features of BBB in vivo including a shear stress environment and the interaction between different types of cells. There is a great demand for a new in vitro platform of BBB that can be used for drug delivery studies. Compared with in vivo models, an in vitro platform has the merits of low cost, shorter test period, and simplicity of operation. Microfluidic technology and microfabrication are good tools in rebuilding the BBB in vitro. During the past decade, great efforts have been made to improve BBB penetration for drug delivery using biochemical or physical stimuli. In particular, compared with other drug delivery strategies, sonoporation is more attractive due to its minimized systemic exposure, high efficiency, controllability, and reversible manner. BBB on chips (BOC) holds great promise when combined with sonoporation. More details and mechanisms such as trans-endothelial electrical resistance (TEER) measurements and dynamic opening of tight junctions can be figured out when using sonoporation stimulating BOC, which will be of great benefit for drug development. Herein, we discuss the recent advances in BOC and sonoporation for BBB disruption with this in vitro platform.

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3D Bioprinting of Neurovascular Tissue Modeling with Collagen‐Based Low‐Viscosity Composites

Wang

Bai

et al. 2023

Adv Healthcare Materials

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In vitro neurovascular unit (NVU) models are valuable for investigating brain functions and developing drugs. However, it remains challenging to recapitulate the native architectural features and ultra‐soft extracellular matrix (ECM) properties of the natural NVU. Cell‐laden bioprinting is promising to prepare complex living tissues, but hard to balance the fidelity and cell growth. This study proposes a novel two‐stage methodology for biomanufacturing functional 3D neurovascular constructs in vitro with low modulus of ECM. At the shaping stage, a low‐viscosity alginate/collagen is printed through an embedded approach; at the culturing stage, the alginate is removed through targeted lysing. The low‐viscosity and rapid crosslinking properties provide a printing resolution of ≈10 µm, and the lysis processing can decrease the hydrogels’ modulus to ≈1 kPa and adjust the porosity of the microstructure, providing cells with an environment closing to the brain ECM. A 3D hollow coaxial neurovascular model is fabricated, in which the endothelial cells has expressed tight junction proteins and shown selective permeability, and the astrocytes outside of the endothelial layer are found to spread out with branches and directly interact with endothelial cells. The present study offers a promising modeling method for better understanding the NVU function and screening neuro‐drugs.

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A 3D printable perfused hydrogel vascular model to assay ultrasound-induced permeability

Abstract: The development of an in vitro model to study vascular permeability is vital for clinical applications such as the targeted delivery of therapeutics. This work demonstrates the use of a...

Cited by 3 publications

References 84 publications

Toward 3D‐Bioprinted Models of the Liver to Boost Drug Development

Toward 3D‐Bioprinted Models of the Liver to Boost Drug Development

Advances in BBB on Chip and Application for Studying Reversible Opening of Blood–Brain Barrier by Sonoporation

3D Bioprinting of Neurovascular Tissue Modeling with Collagen‐Based Low‐Viscosity Composites

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