A 3D-psoriatic skin model for dermatological testing: The impact of culture conditions

Duque-Fernandez, Alexandra; Gauthier, Lydia; Simard, Marie; Jean, Jessica; Gendreau, Isabelle; Morin, Alexandre J. S.; Soucy, Jacques; Auger, Michèle; Pouliot, Roxane

doi:10.1016/j.bbrep.2016.09.012

Cited by 14 publications

(13 citation statements)

References 49 publications

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“…With a growing understanding of the advantages of 3D culture in the field of regenerative medicine, several protocols for the generation of 3D constructs under defined conditions have been developed, mostly for pluripotent stem cells (PSC) and MSC (Al Madhoun et al, ; Bazhanov, Mohammadipoor, & Bartosh, ; Duque‐Fernandez et al, ; Miranda, Fernandes, Diogo, & Cabral, ).…”

Section: Current Alternatives To the Use Of Fbs In Ec Culturementioning

confidence: 99%

“…lated from UCB and grown in 3D collagen matrices. In the presence of commercial platelet lysate, ECFC formed more vascular structures both in vitro and in vivo, as revealed by the quantification of perfused human microvessels, in comparison to ECFC grown in control scaffolds without platelet supplementation.With a growing understanding of the advantages of 3D culture in the field of regenerative medicine, several protocols for the generation of 3D constructs under defined conditions have been developed, mostly for pluripotent stem cells (PSC) and MSC (AlMadhoun et al, 2016;Bazhanov, Mohammadipoor, & Bartosh, 2017;Duque- Fernandez et al, 2016;Miranda, Fernandes, Diogo, & Cabral, 2016).Another humanized large-scale strategy for the propagation of ECFC was proposed byReinisch et al (2009). The cells were successfully isolated from both UCB and PB under XF conditions and further expanded to achieve clinically relevant doses of up to 150 million cells.The proposed XF formulation was based on the standard endothelial growth medium (EGM; basal medium containing GF cocktail), further supplemented with 10% human platelet lysate (HPL) pooled from a minimum of 40 donors, in place of the standard 5% FBS.…”

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confidence: 99%

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Fetal bovine serum-free culture of endothelial progenitor cells-progress and challenges

Bauman

Granja

Barrias

2018

J Tissue Eng Regen Med

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Two decades after the first report on endothelial progenitor cells (EPC), their key role in postnatal vasculogenesis and vascular repair is well established. The therapeutic potential of EPC and their growing use in clinical trials calls for the development of more robust, reproducible, and safer methods for the in vitro expansion and maintenance of these cells. Despite many limitations associated with its usage, fetal bovine serum (FBS) is still widely applied as a cell culture supplement. Although different approaches aiming at establishing FBS-free culture have been developed for many cell types, adequate solutions for endothelial cells, and for EPC in particular, are still scarce, possibly due to the multiple challenges that have to be faced when culturing these cells. In this review, we provide a brief overview on the therapeutic relevance of EPC and critically analyse the available literature on FBS-free endothelial cell culture methods, including xeno-free, serum-free, and chemically defined systems.

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Section: Current Alternatives To the Use Of Fbs In Ec Culturementioning

confidence: 99%

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confidence: 99%

Fetal bovine serum-free culture of endothelial progenitor cells-progress and challenges

Bauman

Granja

Barrias

2018

J Tissue Eng Regen Med

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“…Notably, although epithelial models of the skin or the upper airways belong to the most advanced organ models, publications describing their use for drug testing are scarce. Most studies reporting novel epithelial models conclude that these are promising tools for preclinical testing in vitro [51][52][53] without presenting any data to support this. More studies still have been published with regard to drug delivery approaches [54,55], but without including clear pharmacological readouts.…”

Section: Current Applications Of Human-based Test Models In Pharmacolmentioning

confidence: 99%

3D organ models—Revolution in pharmacological research?

Weinhart

Hocke

Hippenstiel

et al. 2019

Pharmacological Research

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A B S T R A C T 3D organ models have gained increasing attention as novel preclinical test systems and alternatives to animal testing. Over the years, many excellent in vitro tissue models have been developed. In parallel, microfluidic organ-on-a-chip tissue cultures have gained increasing interest for their ability to house several organ models on a single device and interlink these within a human-like environment. In contrast to these advancements, the development of human disease models is still in its infancy. Although major advances have recently been made, efforts still need to be intensified. Human disease models have proven valuable for their ability to closely mimic disease patterns in vitro, permitting the study of pathophysiological features and new treatment options. Although animal studies remain the gold standard for preclinical testing, they have major drawbacks such as high cost and ongoing controversy over their predictive value for several human conditions. Moreover, there is growing political and social pressure to develop alternatives to animal models, clearly promoting the search for valid, cost-efficient and easy-to-handle systems lacking interspecies-related differences.In this review, we discuss the current state of the art regarding 3D organ as well as the opportunities, limitations and future implications of their use.

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“…Target identification and validation remain a pressing challenge in the pharmaceutical industry [79] even for psoriasis. In that context, the use of either non-diseased [85] or diseased [86] human skin appears preferable for drug testing as almost all available full-thickness skin equivalents, despite the addition of immune cells, remain at risk of being artificial, [87] since the available models may not express the genes characteristic for human psoriatic skin. Healthy human skin, lesional and uninvolved skin samples have been characterized.…”

Section: The Assessment Of Target Organ Pk and Pd Interactionsmentioning

confidence: 99%

Translational drug discovery and development with the use of tissue‐relevant biomarkers: Towards more physiological relevance and better prediction of clinical efficacy

Florian

Flechsenhar

Bartnik

et al. 2019

Experimental Dermatology

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Due to the clinical development of drugs such as secukinumab, ustekinumab and dupilumab, major changes have been achieved in the treatment of patients diagnosed with psoriasis and atopic dermatitis. In academia and the pharmaceutical industry, research is increasingly moving towards the development of bispecific antibodies and multi‐specific nanobodies, as there is a compelling need for new treatment modalities for patients suffering from autoimmune or malignant disease. The purpose of this review is to discuss aspects of translational drug development with a particular emphasis on indications such as psoriasis and atopic dermatitis. The identification of biomarkers, the assessment of target organ pharmacokinetic and pharmacodynamics interactions and a wide range of in vitro, ex vivo and in vivo models should contribute to an appropriate prediction of a biological effect in the clinical setting. As human biology may not be perfectly reflected by approaches such as skin equivalents or animal models, novel approaches such as the use of human skin and dermal microperfusion assays in healthy volunteers and patients appear both reasonable and mandatory. These models may indeed generate highly translationally relevant data that have the potential to reduce the failure rate of drugs currently undergoing clinical development.

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A 3D-psoriatic skin model for dermatological testing: The impact of culture conditions

Cited by 14 publications

References 49 publications

Fetal bovine serum-free culture of endothelial progenitor cells-progress and challenges

Fetal bovine serum-free culture of endothelial progenitor cells-progress and challenges

3D organ models—Revolution in pharmacological research?

Translational drug discovery and development with the use of tissue‐relevant biomarkers: Towards more physiological relevance and better prediction of clinical efficacy

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