A 5‐color flow cytometric method for extended 8‐part leukocyte differential

Abstract

Here a new cytometric differential is proposed with a robust gating strategy which may be used even by unskilled cytometrists and can be easily automated. © 2017 International Clinical Cytometry Society.

Issue Highlights ‐ November 2017 (92:B6)

“…Guy, et al utilize a 5‐color approach to get a desired 8‐part leukocyte differential. Some elements were derived from a 10‐color immunophenotyping technique and from an 8‐color protocol.…”

“…Some elements were derived from a 10‐color immunophenotyping technique and from an 8‐color protocol. The new cytometric differential with a promising gating strategy is presented, the system lends itself to convert to automated differentials .…”

Issue Highlights – November 2019

“…In this century, analytical capabilities are constantly improving. Just in this decade eight-part differential gating strategies for leukocytes are becoming routine clinical tools (2). Fine-tuning of valid cost-effective multi-analyte methods vs. solo assay's sensitivity/specificity are continuously realigned to deliver better care (3)(4)(5).…”

“…Delineation of normal/activated state and identification of abnormal populations is critical (7). Such constantly growing polychromatic assay protocols require realigned baseline assays different from the more traditional 3-4 color MAb combinations (2,(8)(9)(10)(11).…”