A‐549 is a suitable cell line for primary isolation of coxsackie B viruses

Abstract

A common receptor for coxsackie B virus and adenovirus has been described recently in cells of human and murine origin. Since the established cell line A-549 is suitable for adenoviruses, the potential use of A-549 cells for the isolation of coxsackie B viruses from clinical samples was investigated. All throat swabs sent to the laboratory between April 1998 and June 1999 were inoculated onto monolayers of MRC-5 and A-549 cells in tubes, and the enterovirus isolates obtained were typed. From April to June 1999, A-549 cells were compared prospectively to Buffalo green monkey (BGM) cells, considered as the most susceptible cell line for isolating coxsackie B viruses. Fifty-six out of 171 enterovirus isolates (33%) displayed a cytopathic effect (CPE) in the A-549 monolayer only, 48 isolates (28%) in the MRC-5 monolayer only, and 67 isolates (39%) in both cell lines. Most isolates that showed CPE in A-549 cells only (48 out of 56, 86%) were coxsackie B viruses, belonging to four different serotypes (B1, B2, B4, and B6). When BGM and A-549 cells were inoculated in parallel, both recovered the same number of coxsackie B isolates (n = 20), and the CPE was noted on approximately the same day. In conclusion, growth in A-549 but not MRC-5 cells identified coxsackie B viruses in most cases. A-549 was comparable to BGM for primary isolation of coxsackie B viruses.

Comparison of multiple shell vial cell lines for isolation of enteroviruses: A national perspective

“…Interestingly, Otero et al (2001) found that when inoculated in parallel, A549 and BGM cells isolated the same number of coxsackievirus group B isolates (n = 20), and compared to MRC-5, A549 isolated an additional 48 cases of coxsackievirus B, including serotypes B1, B2, B4 and B6. Kok et al (1998) also noted adequate sensitivity with A549 cells in which 25 (64%) of 39 positive enterovirus isolates were recovered from A549 cells.…”

First epidemic of aseptic meningitis due to echovirus type 13 among Spanish children

“…5%) were received in the National Reference Laboratory for Poliomyelitis and Enteroviruses (CNM, ISCIII) from 15 different Spanish primary virological laboratories within 11 Autonomous Communities (AACCs). Viral isolation was performed on human rhabdomyosarcoma cells (RD), human embryonic fibroblast cells (HEF) and human lung carcinoma cells (A-549), selected for their support of EV replication [20,21]. All isolates were studied by immunoflorescence using monoclonal mouse anti-enterovirus antibodies (clone 5-D8/1, Dako, Glostrup, Denmark).…”

Διερεύνηση Του Ρόλου Της Φυσικής Ανοσίας Στην Παθογένεια Λοιμώξεων Με Εντεροΐούς

“…reshus, cynomolgus και Buffalo green) και κάποιες ανθρώπινες κυτταρικές σειρές (π.χ. HeLa, Hep-2, A549, CaCo2 και NCI-H292) έχουν καλή ευαισθησία για την ανίχνευση των CVBs [370,371]. Αντίθετα, οι κυτταρικές σειρές HK, HELF, HK και RD δεν είναι ιδανικές για την ανίχνευση των CVBs (Romero 2006).…”