A bacteriophage typing system for <i>Rhizobium meliloti</i>

Lesley, S. M.

doi:10.1139/m82-024

Cited by 42 publications

(14 citation statements)

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“…Phages were stored in YEM broth which contained 5% chloroform at 4°C. The phage typing assay described by Lesley (1982) was used for testing the phage host range to bacterial cultures cultivated by a two-layer method on YEM agar.…”

Section: Bacterial Strainsmentioning

confidence: 99%

Phage sensitivity and host range of Rhizobium strains isolated from root nodules of temperate legumes

1993

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Twenty-five Rhizobium strains were isolated from root nodules of Astragalus spp. (lo), Hedysarum alpinum (7), Glycyrrhiza pallidiflora (3) and Ononis arvensis (5). The sensitivity of these strains to bacteriophages of Rhizobium loti, R. meliloti, R. galegae and R. leguminosarum was studied. Phages specific to R. loti strains were shown to induce the phage lysis of several Astragalus, Hedysarum and Ononis rhizobia. Ten R. loti strains tested for nodulation abilities on the plant hosts under investigation were able to develop nitrogen-fixing nodules on the Ononis arvensis roots. On the other hand, rhizobia from Ononis and Glycyrrhiza could form an effective symbiosis with Lotus corniculatus plants, so these bacteria are considered to belong to the Rhizobium loti taxon. Bacterial strains isolated from Astragalus and Hedysarum were observed to cross-nodulate their plant hosts as well as Oxytropis campestris, Glycyrrhiza uralensis and Ononis arvensis plants, whereas they could not nodulate Lotus plants. It is concluded that these Rhizobium strains comprise a cross-inoculation group related to Rhizobium loti.

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Section: Bacterial Strainsmentioning

confidence: 99%

Phage sensitivity and host range of Rhizobium strains isolated from root nodules of temperate legumes

1993

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“…The occurrence and distribution of phages active against various groups of rhizobia have been reported in India (Dhar et al, 1980, Dhar et al, 1993, Singh et al, 1980, Jaiswal and Dhar, 2010), Poland (Staniewski, 1970), New Zealand (Patel, 1976), Canada (Lesley, 1982) and Senegal (Lajudie and Bogusz, 1984). Recently, phages infective on common bean rhizobia have also been isolated and characterized in Mexico (Santamaría et al, 2014).…”

Section: Introductionmentioning

confidence: 97%

Identification and characterization of phages parasitic on bradyrhizobia nodulating groundnut ( Arachis hypogaea L.) in South Africa

Msimbira¹,

Jaiswal

Dakora

2016

Applied Soil Ecology

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“…P hage s ensitivit y p att ern has b een suggest ed as one of t he powerf ul m ethods t o differentiate betw een bact eria acc ording t o their genot ypic and phenot ypic tr aits (Lindstrom and Leht omaki, 1988;Thurman and Brom field, 1988). In addition, it giv es fast er and r eproducible r esul ts f or discriminating of i ndigenous rhiz obial isol at es and prov es us eful enough for m onitoring the purity of t he bact erial g ermplasm (Lesley, 1982;Lindstr om et al , 1983;Appunu and Dhar, 2006).…”

Section: Arti Cle Code: Introductionmentioning

confidence: 99%

Efficiency of nitrogen fixation by lysogenic and non-lysogenic isolates of Rhizobium nodulated Sesbania aegyptica.

Hashem¹

2017

Egypt. J. Exp. Biol. (Bot.)

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Efficiency of nitrog en fi xation by ly sogenic and non -ly sogeni c isolates of Rhiz obium nodul ated Se sbani a aeg yptica ABSTRACT:Six Sesbania nodulating rhizobia designated as (RS1, RS2, RS3, RS4, RS5, and RS6) isolated from El-Garbia and El-Menoufia governorates were characterized. These isolates were fast growing Rhizobium and produced Indole acetic acid with different concentrations. Furthermore, all these isolates were varied in their response to different concentrations of NaCl and Intrinsic antibiotic resistance pattern. Lysogenicity in the isolated Sesbania nodulating rhizobia was tested. One lysogenic isolate RS2 (RSL) was identified among the tested six Sesbania Rhizobium, this isolate produced turbid plaques. Single plaque was picked and propagated and designated as (ϕB5). Electron microscopy results suggested that ϕB5 belong to family Myoviridae having icosahedral head (65.27 ± 5.09 nm), long contractile tail (91.4 ± 1.3, 17.93 ± 1.25 nm) and neck (21.0 ± 1.3, 4.2 ± 0.0 nm) and has approximately 23.5 Kbp of dsDNA. Further incubation time of lytic area performed by plating of RSL with ϕB5 lead to appearance of resistant Rhizobium colony (RP5). This purified colony was non-lysogenic Rhizobium. The symbiotic interaction of RSL (phage sensitive) and RP5 (phage resistance) was studied using Sesbania aegyptica as a host in presence and absence of phages. Assay of symbiotic efficiency based on nodule number, nitrogenase activity, dry weight and total nitrogen of shoot system was detected. Symbiotic effectiveness in phage resistant nonlysogenic isolate was higher than in lysogenic phage sensitive isolate in presence of phages in soil. The present study showed a significant relationship between the phage sensitivity and symbiotic efficiency of the isolated Rhizobium. KEY WORDS:Nitrogen fixation, lysogenic and non-lysogenic isolates, Rhizobium nodulated Sesbania aegyptica CORRESP ONDENCE: E m an A . M. H at em Botany department,

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A bacteriophage typing system for Rhizobium meliloti

Cited by 42 publications

References 0 publications

Phage sensitivity and host range of Rhizobium strains isolated from root nodules of temperate legumes

Phage sensitivity and host range of Rhizobium strains isolated from root nodules of temperate legumes

Identification and characterization of phages parasitic on bradyrhizobia nodulating groundnut ( Arachis hypogaea L.) in South Africa

Efficiency of nitrogen fixation by lysogenic and non-lysogenic isolates of Rhizobium nodulated Sesbania aegyptica.

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