2009
DOI: 10.1128/iai.01226-08
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A Baculovirus Dual Expression System-Based Malaria Vaccine Induces Strong Protection againstPlasmodium bergheiSporozoite Challenge in Mice

Abstract: We have previously shown that a recombinant baculovirus that displays Plasmodium berghei circumsporozoite protein (PbCSP), a homolog of the leading human malaria vaccine candidate, on the viral envelope protected 60% of mice against P. berghei infection. Here, we describe a second-generation baculovirus vaccine based on the "baculovirus dual expression system," which drives PbCSP expression by a dual promoter that consists of tandemly arranged baculovirus-derived polyhedrin and mammal-derived cytomegalovirus p… Show more

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Cited by 51 publications
(48 citation statements)
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“…Similarly, the BALB/c (H-2K d ) mice we used here may not be able to recognize the CTL epitope on PvCSP. Therefore, anti-PvCSP Abs induced by BDESPvs25-PvCSP-G could theoretically make a significant contribution to protection against infections naturally transmitted by sporozoites, although we cannot fully discount the possibility of a role for cellular immune responses in immune protection, as baculoviral vectors have the potential to induce CTLs via stimulation by innate immunity (12).…”
Section: Discussionmentioning
confidence: 99%
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“…Similarly, the BALB/c (H-2K d ) mice we used here may not be able to recognize the CTL epitope on PvCSP. Therefore, anti-PvCSP Abs induced by BDESPvs25-PvCSP-G could theoretically make a significant contribution to protection against infections naturally transmitted by sporozoites, although we cannot fully discount the possibility of a role for cellular immune responses in immune protection, as baculoviral vectors have the potential to induce CTLs via stimulation by innate immunity (12).…”
Section: Discussionmentioning
confidence: 99%
“…Three recombinant baculoviruses were generated in Sf9 cells by cotransfection of the corresponding plasmid vector with BacVector-2000 DNA (Merck Millipore, Guyancourt, France) following the manufacturer's protocol. Purification of viral particles was performed as described previously (12).…”
Section: Methodsmentioning
confidence: 99%
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