A barley mutant with improved salt tolerance through ion homeostasis and ROS scavenging under salt stress

Kiani, Davood; Soltanloo, Hassan; Ramezanpour, S. Sanaz; Qumi, Ali Asghar Nasrolahnezhad; Yamchi, Ahad; Nezhad, Khalil Zaynali; Tavakol, Elahe

doi:10.1007/s11738-017-2359-z

Cited by 19 publications

(11 citation statements)

References 46 publications

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“…six hours after exposure to the salt stress. The results of this study were similar to report of Kiani et al, [29]. Furthermore, the rate of evaporation via transpiration stream and H 2 O 2 production via photosystems (I and II) were reduced due to stomatal closure, which is an important mechanism of salt tolerance [90,16].…”

Section: Plos Onesupporting

confidence: 91%

“…Two barley (Hordeum vulgare L.) genotypes including a salt-tolerant mutant line "M4-73-30" and its wild-type cultivar "Zarjou" were used for the present study. These genotypes had been provided by Seed and Plant Improvement Institute, Karaj, Iran [29,30]. A salt-tolerant mutant genotype was produced by the gamma irradiation approach and evaluated for salt tolerance through field trials.…”

Section: Plant Materials and Salt Stress Conditionsmentioning

confidence: 99%

“…This genotype had been previously introduced as a salt-tolerant cultivar called "Roodasht" by Seed and Plant Improvement Institute, Karaj, Iran [31]. Previous studies [29,31,30] revealed that a salt-tolerant mutant had a higher ability to tolerate salt stress than its wild-type genotype by physiological response related to salinity tolerance.…”

Section: Plant Materials and Salt Stress Conditionsmentioning

confidence: 99%

“…The total RNAs were extracted separately from the root and shoot samples by the Biozol method (BioFlax, Japan) [29]. The RNA quality was checked by 1% agarose gel electrophoresis and 30 μg was used for RNA-Seq.…”

Section: Rna Isolationmentioning

confidence: 99%

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The RNA-seq transcriptomic analysis reveals genes mediating salt tolerance through rapid triggering of ion transporters in a mutant barley

et al. 2020

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Considering the complex nature of salinity tolerance mechanisms, the use of isogenic lines or mutants possessing the same genetic background albeit different tolerance to salinity is a suitable method for reduction of analytical complexity to study these mechanisms. In the present study, whole transcriptome analysis was evaluated using RNA-seq method between a salt-tolerant mutant line "M4-73-30" and its wild-type "Zarjou" cultivar at seedling stage after six hours of exposure to salt stress (300 mM NaCl). Transcriptome sequencing yielded 20 million reads for each genotype. A total number of 7116 transcripts with differential expression were identified, 1586 and 1479 of which were obtained with significantly increased expression in the mutant and the wild-type, respectively. In addition, the families of WRKY, ERF, AP 2 /EREBP, NAC, CTR/DRE, AP 2 /ERF, MAD, MIKC, HSF, and bZIP were identified as the important transcription factors with specific expression in the mutant genotype. The RNA-seq results were confirmed at several time points using qRT-PCR for some important salt-responsive genes. In general, the results revealed that the mutant accumulated higher levels of sodium ion in the root and decreased its transfer to the shoot. Also, the mutant increased the amount of potassium ion leading to the maintenance a high ratio [K + ]/ [Na + ] in the shoot compared to its wild-type via fast stomata closure and consequently transpiration reduction under the salt stress. Moreover, a reduction in photosynthesis and respiration was observed in the mutant, resulting in utilization of the stored energy and the carbon for maintaining the plant tissues, which is considered as a mechanism of salt tolerance in plants. Up-regulation of catalase, peroxidase, and ascorbate peroxidase genes has resulted in higher accumulation of H 2 O 2 in the wild-type compared to the mutant. Therefore, the wild-type initiated rapid ROS signals which led to less oxidative scavenging in comparison with the mutant. The mutant increased expression in the ion transporters and the channels related to the salinity to maintain the ion homeostasis. In overall, the results demonstrated that the mutant responded better to the salt stress under both osmotic and

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Section: Plos Onesupporting

confidence: 91%

Section: Plant Materials and Salt Stress Conditionsmentioning

confidence: 99%

Section: Plant Materials and Salt Stress Conditionsmentioning

confidence: 99%

Section: Rna Isolationmentioning

confidence: 99%

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The RNA-seq transcriptomic analysis reveals genes mediating salt tolerance through rapid triggering of ion transporters in a mutant barley

et al. 2020

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“…Many studies have shown that endogenous GSH is the main player in coordinating the activation of antioxidant defense and glyoxalase systems, and applications of exogenous GSH have been reported to promote plant growth and improve stress tolerance by detoxifying stressinduced ROS (Mostofa et al 2014;Wang et al 2014;Cao et al 2015;Nahar et al 2015a, b;Ding et al 2016). The maintenance of a high K + concentration and low Na + /K + ratio in the cytosol is also critical for salt tolerance (Kiani et al 2017). Our results also showed that application of exogenous GSH was more effective in reducing the contents of Na + and Cland the ratio of Na + /K + , and mitigating the effects of salt stress on the growth of plants and P n , G s and C i as was found in cotton (Ibrahim et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Exogenous GSH protects tomatoes against salt stress by modulating photosystem II efficiency, absorbed light allocation and H2O2-scavenging system in chloroplasts

Zhou

Diao

Cui

et al. 2018

Journal of Integrative Agriculture

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The effects of exogenous GSH (reduced glutathione) on photosynthetic characteristics, photosystem II efficiency, absorbed light energy allocation and the H 2 O 2-scavenging system in chloroplasts of salt-stressed tomato (Solanum lycopersicum L.) seedlings were studied using hydroponic experiments in a greenhouse. Application of exogenous GSH ameliorated saline-induced growth inhibition, the disturbed balance of Na + and Cl − ions and Na + /K + ratios, and the reduction of the net photosynthetic rate (P n). GSH also increased the maximal photochemical efficiency of PSII (F v /F m), the electron transport rate (ETR), the photochemical quenching coefficient (q P), and the non-photochemical quenching coefficient (NPQ). In addition, GSH application increased the photochemical quantum yield (Y(II)) and relative deviation from full balance between the photosystems (β/α-1) and decreased the PSII excitation pressure (1-q P) and quantum yield of non-regulated energy dissipation (Y(NO)) in leaves of salt-stressed tomatoes without BSO (L-buthionine-sulfoximine, an inhibitor of key GSH synthesis enzyme γ-glutamylcysteine synthetase) or with BSO. Further, the addition of GSH depressed the accumulation of H 2 O 2 and malondialdehyde (MDA), induced the redistribution of absorbed light energy in PSII reaction centers, and improved the endogenous GSH content, GSH/GSSH ratio and activities of H 2 O 2-scavenging enzymes (including superoxidase dismutase (SOD), catalase (CAT), peroxidase (POD) and key enzymes in the AsA-GSH cycle and Grx system) in the chloroplasts of salt-stressed plants with or without BSO. Therefore, GSH application alleviates inhibition of salt-induced growth and photosynthesis mainly by overcoming stomatal limitations, improving the PSII efficiency, and balancing the uneven distribution of light energy to reduce the risk of ROS generation and to mediate chloroplast redox homeostasis and the antioxidant defense system to protect the chloroplasts from oxidative damage. Thus, GSH may be used as a potential tool for alleviating salt stress in tomato plants.

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Genetic Diversity for Barley Adaptation to Stressful Environments

Fricano¹,

Battaglia²,

Mica³

et al. 2021

Genomic Designing for Abiotic Stress Resistant Cereal Crops

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A barley mutant with improved salt tolerance through ion homeostasis and ROS scavenging under salt stress

Cited by 19 publications

References 46 publications

The RNA-seq transcriptomic analysis reveals genes mediating salt tolerance through rapid triggering of ion transporters in a mutant barley

The RNA-seq transcriptomic analysis reveals genes mediating salt tolerance through rapid triggering of ion transporters in a mutant barley

Exogenous GSH protects tomatoes against salt stress by modulating photosystem II efficiency, absorbed light allocation and H2O2-scavenging system in chloroplasts

Genetic Diversity for Barley Adaptation to Stressful Environments

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