A benzopyrylium dye as a novel effective cationic uncoupler of oxidative phosphorylation

Abstract

Benzopyryhumdye BDBP was found to be an effective uncoupler of oxidative phosphorylation It stimulates state 4 respiration of mitochondria 2-fold at about 2 PM, reveals a maximum stimulation (&fold) at 30 PM and inhibits the respiration at htgher concentrattons. BDBP is also an effecttve proton (hydroxyl) carrier through bilayer lipid membranes (BLM) as ts seen from the electrtcal properties of BLM with BDBP. Picrate enhances the effects of BDBP on the mttochondnal resptratton and BLM conductance. The BDBP activtttes arc accounted for by formation of dimers and complexes with ptcrate.