A bio-inspired plasmonic nanosensor for angiotensin-converting enzyme through peptide-mediated assembly of gold nanoparticles

Su, Shixuan; Yu, Ting; Hu, Jing; Xianyu, Yunlei

doi:10.1016/j.bios.2021.113621

Cited by 21 publications

(11 citation statements)

References 32 publications

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“…Figure 6 A shows the dependence of inhibition efficiency on enalaprilat concentration. The half-maximal inhibitory concentration (IC 50 ) was found to be 78 nM, which is consistent with that obtained by other methods [ 34 ]. We also monitored the current change for measuring a fixed concentration of thrombin in the presence of different concentrations of argatroban.…”

Section: Resultssupporting

confidence: 89%

A General, Label-Free and Homogeneous Electrochemical Strategy for Probing of Protease Activity and Screening of Inhibitor

et al. 2022

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Proteases play a critical role in regulating various physiological processes from protein digestion to wound healing. Monitoring the activity of proteases and screening their inhibitors as potential drug molecules are of great importance for the early diagnosis and treatment of many diseases. In this work, we reported a general, label-free and homogeneous electrochemical method for monitoring protease activity based on the peptide–copper interaction. Cleavage of peptide substrate results in the generation of a copper-binding chelator peptide with a histidine residue in the first or third position (His1 or His3) at the N-terminal. The redox potential and current of copper coordinated with the product are different from the free copper or the copper complex with the substrate, thus allowing for the detection of protease activity. Angiotensin-converting enzyme (ACE) and thrombin were determined as the model analytes. The label-free and homogeneous electrochemical method can be used for screening protease inhibitors with high simplicity and sensitivity.

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Section: Resultssupporting

confidence: 89%

A General, Label-Free and Homogeneous Electrochemical Strategy for Probing of Protease Activity and Screening of Inhibitor

et al. 2022

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“…Similar attempts were also reported for the detection of other metabolite biomarkers, including amino acids (e.g., cysteine), [106,107] lipids (e.g., cholesterol), [101][102][103] vitamins F I G U R E  (A) Postoperative brain infection diagnosis based on glucose quantitation. The accurate quantitation of glucose was achieved by a plasmonic silver nanoshell-assisted LDI MS. Reproduced with permission.…”

Section: Serum Gynecological Cancermentioning

confidence: 70%

Nanomaterials‐assisted metabolic analysis toward in vitro diagnostics

2022

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In vitro diagnostics (IVD) has played an indispensable role in healthcare system by providing necessary information to indicate disease condition and guide therapeutic decision. Metabolic analysis can be the primary choice to facilitate the IVD since it characterizes the downstream metabolites and offers real‐time feedback of the human body. Nanomaterials with well‐designed composition and nanostructure have been developed for the construction of high‐performance detection platforms toward metabolic analysis. Herein, we summarize the recent progress of nanomaterials‐assisted metabolic analysis and the related applications in IVD. We first introduce the important role that nanomaterials play in metabolic analysis when coupled with different detection platforms, including electrochemical sensors, optical spectrometry, and mass spectrometry. We further highlight the nanomaterials‐assisted metabolic analysis toward IVD applications, from the perspectives of both the targeted biomarker quantitation and untargeted fingerprint extraction. This review provides fundamental insights into the function of nanomaterials in metabolic analysis, thus facilitating the design of next‐generation diagnostic devices in clinical practice.

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“…27 When the particle spacing is smaller than the average particle size of Au NPs, the color will change from red to blue, which can be seen by the naked eye. 28 The surface of Au NPs can be modified by recognition molecules such as antibodies, 29 antibiotics, and aptamers 30,31 to form functionalized Au NPs that can specifically bind with bacteria. The presence of targeted bacteria can result in the aggregation of Au NPs and a visible color change of the solution, allowing for the quantitative detection through the UV−vis absorption spectrum.…”

Section: Photothermal Effectmentioning

confidence: 99%

Surface Chemistry of Gold Nanoparticles for Bacterial Detection and Antimicrobial Applications

Zhang

Ding

et al. 2023

ACS Materials Lett.

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Bacteria can infect the human body and cause disease, which endangers public safety. The emergence of multidrug-resistant bacteria poses a great challenge on the treatment of bacterial infections. Quickly detecting pathogenic bacteria and effectively killing them are of great significance for the targeted treatment of bacterial infections. On one hand, gold nanoparticles (Au NPs) are widely used for the rapid detection of bacteria, because of their surface plasmon resonance properties that allow for a visible readout based on a color change. On the other hand, functionalized Au NPs with versatile surface modifications can show good bactericidal effects. The surface chemistry of Au NPs can be tailored for dual functionality that integrates bacterial detection and antibacterial activity. The dual-functional Au NPs can greatly shorten the interval between detection and treatment, which is beneficial for the early inhibition of bacterial growth. In this Review, we focus on the strategies for bacterial detection and antibacterial applications based on the surface chemistry of Au NPs. We discuss recent advances and prospects in the application of dual-functional Au NPs with bacterial detection and antibacterial features.

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A bio-inspired plasmonic nanosensor for angiotensin-converting enzyme through peptide-mediated assembly of gold nanoparticles

Cited by 21 publications

References 32 publications

A General, Label-Free and Homogeneous Electrochemical Strategy for Probing of Protease Activity and Screening of Inhibitor

A General, Label-Free and Homogeneous Electrochemical Strategy for Probing of Protease Activity and Screening of Inhibitor

Nanomaterials‐assisted metabolic analysis toward in vitro diagnostics

Surface Chemistry of Gold Nanoparticles for Bacterial Detection and Antimicrobial Applications

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