A Bioassay for the Quantitation of Cutaneous Reactions Associated with Pink-Rot Celery

Perone, Vernon B.; Scheel, Lester D.; Meitus, Robert J.

doi:10.1038/jid.1964.60

Cited by 18 publications

(6 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Xanthotoxin and bergapten were produced only in the rotted areas. Coinciding with the results of Perone et al (6), neither was found in healthy portions of the same stalk.…”

Section: Methodssupporting

confidence: 87%

“…Identification was confirmed by comparison of the ultraviolet spectra of the eluted TLC spots with reference spectra (9). The identity of the compounds was further established by the production of a photosensitized erythemal reaction (6) on rabbit skin by using an ethyl acetate solution of the suspected phototoxin isolation by preparative TLC.…”

Section: Methodsmentioning

confidence: 98%

“…Sclerotinia sclerotiorum attacks many kinds of fruits and vegetables in the field as well as in storage, in transit, and in the market (2,8). Sclerotinia-diseased celery (pink rot) was found to cause a blistering cutaneous disease on the skin of people working in celery fields (1,6). The skin-sensitizing phototoxins xanthotoxin (8-methoxypsoralen), 4,5', 8-trimethylpsoralen and bergapten (5-methoxypsoralen) have been reported in diseased celery (3,5,6,9).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Isolation and Identification of Xanthotoxin (8-Methoxypsoralen) and Bergapten (5-Methoxypsoralen) from Celery Infected with Sclerotinia sclerotiorum

Koehler

Ayres

1972

Applied Microbiology

View full text Add to dashboard Cite

Methods for the production, isolation, and identification of xanthotoxin and bergapten from celery diseased by Sclerotinia sclerotiorum (Libert) de Bary were investigated. The only conditions under which this mold was capable of producing xanthotoxin and bergapten occurred when the mold was actively growing on fresh (metabolizing) celery. Neither compound was found in uninfected celery, in the mold growing on nutrient media, on nutrient media fortified with 30% filter-sterilized celery juice, or on nonmetabolizing celery. Maximal xanthotoxin production of 320 pg per g of dry rotted celery occurred at 20 C, although mycelial growth increased until 30 C. Neither xanthotoxin nor bergapten was found when the mold grew on 11 agricultural commodities other than celery.

show abstract

“…Xanthotoxin and bergapten were produced only in the rotted areas. Coinciding with the results of Perone et al (6), neither was found in healthy portions of the same stalk.…”

Section: Methodssupporting

confidence: 87%

Section: Methodsmentioning

confidence: 98%

mentioning

confidence: 99%

See 1 more Smart Citation

Isolation and Identification of Xanthotoxin (8-Methoxypsoralen) and Bergapten (5-Methoxypsoralen) from Celery Infected with Sclerotinia sclerotiorum

Koehler

Ayres

1972

Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…The F-2 crystals were shown to have the same biologic activity (1.5 pg/sq in. = 14reaction) (Perone et al, 1963) as the 8-methoxypsoralen.…”

Section: Methodsmentioning

confidence: 99%

“…This plant disease has been shown to be caused by the growth of a fungus, Sclerotinia sclerotiorum, on the celery stalks, usually starting near the soil line. By biological assay Perone et al (1963) have shown that only the diseased portions of the plants contain products which, when placed on the skin and exposed to sunlight or ultraviolet radiation of 3200-3700 A with a peak at 3650 A, produce the blistering type of lesion characteristically seen in celery harvesters. This paper will describe the procedure used to isolate and characterize two phototoxic psoralens from diseased celery tissue.…”

mentioning

confidence: 99%