A biomimetic hybrid material consisting of CaCO₃ mesoporous microspheres and an alternating copolymer for reversed-phase HPLC

Abstract

Inspired by egg shells, amphiphilic copolymer-modified monodisperse mesoporous CaCO3 microspheres were applied as a separation material.

Amphiphilic Copolymer-Modified Eggshell-Based Column Packing Materials for the Preparative Separation of Basic Drugs

“…Column packing materials for preparative HPLC need to be obtained inexpensively because they are used in large quantities. Our reversed-phase packing material based on calcium carbonate microspheres , exhibited high separation ability for basic compounds, but there was a limit in the amount that could be synthesized while maintaining uniform particle size and shape, making scale-up difficult. Furthermore, the use of relatively expensive poly­(sodium 4-sulfonate) (PSS) to construct mesostructured particles and the lack of high synthetic yields made them unsuitable for use in large-scale preparative HPLC columns.…”

“…20−23 We previously developed an alkali-resistant reversed-phase column packing material based on monodisperse mesoporous calcium carbonate microspheres modified with poly(maleic acid-alt-1octadecene) (CaCO 3 -PMAcO). 24,25 Since calcium carbonate is a basic oxide, the CaCO 3 -PMAcO column showed better stability than octadecyl-modified silica (ODS) columns when used with an alkaline mobile phase. In addition, the CaCO 3 -PMAcO column achieved a separation performance comparable to that of ODS columns and showed potential for the practical analysis of basic drugs under alkaline condition.…”

“…The use of an alkaline mobile phase to prevent the ionization of basic compounds is undesirable because it reduces the durability of silica gel-based packing materials. Many studies have been performed to solve the poor separation performance of basic compounds owing to adsorption on the residual silanol groups. − We previously developed an alkali-resistant reversed-phase column packing material based on monodisperse mesoporous calcium carbonate microspheres modified with poly­(maleic acid- alt -1-octadecene) (CaCO 3 -PMAcO). , Since calcium carbonate is a basic oxide, the CaCO 3 -PMAcO column showed better stability than octadecyl-modified silica (ODS) columns when used with an alkaline mobile phase. In addition, the CaCO 3 -PMAcO column achieved a separation performance comparable to that of ODS columns and showed potential for the practical analysis of basic drugs under alkaline condition .…”

Inorganic Porous Bulk Discs as a Matrix for Thin-Layer Chromatography and Translucent Hard Composite Materials

“…38 Recent studies show that CaCO 3 with hierarchical structures can be developed as new stationary phases for chromatography. 19,39 Calcium phosphate materials, particularly hydroxyapatite, have been used as columns for protein separation and purification. 40,41 In this work, nanosized Mg-ACC, MCSH, and HAp were applied as the stationary phases and water as the solvent.…”

“…The CaCO 3 -based material has been used for chromatography since 100 years ago . Recent studies show that CaCO 3 with hierarchical structures can be developed as new stationary phases for chromatography. , Calcium phosphate materials, particularly hydroxyapatite, have been used as columns for protein separation and purification. , In this work, nanosized Mg-ACC, MCSH, and HAp were applied as the stationary phases and water as the solvent. The investigation of adsorption properties of biomolecules on these materials is important due to their relevance to biomineralization processes .…”