Encyclopedia of Life Sciences 2017
DOI: 10.1002/9780470015902.a0027015
|View full text |Cite
|
Sign up to set email alerts
|

A Biophysical Toolkit for Molecular Interactions

Abstract: Intermolecular interactions inside and around the cell are key to maintain basic cell homeostasis and to sense and react to external stimuli. Moreover, the action of therapeutics and other xenobiotics occurs through physical interactions of these agents with biomolecules present in the cell or tissue, thereby interfering with the ‘native’ interactions of the biomolecules. Biophysical characterisation of molecular interactions is a bottom‐up reductionist approach that implies measuring the physicochemical prope… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2

Citation Types

0
8
0

Year Published

2019
2019
2024
2024

Publication Types

Select...
5
1

Relationship

0
6

Authors

Journals

citations
Cited by 6 publications
(8 citation statements)
references
References 57 publications
0
8
0
Order By: Relevance
“…Multiple genes encoded for ribosomal protein were downregulated in the QS-deficient mutant. Though many ribosomal proteins are not essential in translation, these proteins could increase the integrity of ribosomes [81]. Other than protein synthesis, ribosomal proteins also act as transcriptional factors to regulate their own or other ribosomal proteins’ transcriptional level [81].…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Multiple genes encoded for ribosomal protein were downregulated in the QS-deficient mutant. Though many ribosomal proteins are not essential in translation, these proteins could increase the integrity of ribosomes [81]. Other than protein synthesis, ribosomal proteins also act as transcriptional factors to regulate their own or other ribosomal proteins’ transcriptional level [81].…”
Section: Discussionmentioning
confidence: 99%
“…Though many ribosomal proteins are not essential in translation, these proteins could increase the integrity of ribosomes [81]. Other than protein synthesis, ribosomal proteins also act as transcriptional factors to regulate their own or other ribosomal proteins’ transcriptional level [81]. Additionally, they were also found to control transcription termination [82].…”
Section: Discussionmentioning
confidence: 99%
“…Paralleling the rise of biophysical methods in drug discovery, biophysical instrumentation has also in recent years improved in speed, sensitivity, robustness, and dynamic range, delivering rigorous, reliable, high-definition, and information-rich data. This field is constantly evolving to improve the drug discovery process and to address the new challenges of the next-generation therapeutics (Cariuk et al 2013;Renaud et al 2016;Santiveri et al 2017).…”
Section: Introductionmentioning
confidence: 99%
“…enzyme-linked immunosorbent assay (ELISA) and coimmunoprecipitation, whereas biophysical methods comprise for instance fluorescence polarisation, surface plasmon resonance (SPR), isothermal titration calorimetry (ITC) and microscale thermophoresis (MST). [3][4][5][6] The determination of several interaction parameters is favourable, but this can lead to complex and time-consuming experiments. In many cases, evaluation of bimolecular interactions can be sufficiently and more easily performed by only measuring K D .…”
Section: Introductionmentioning
confidence: 99%