A Biosensor Based on Polyaniline‐Carbon Nanotube Core‐Shell for Electrochemical Detection of Pesticides

Abstract

A core-shell structure based on polyaniline (PANI) and multiwalled carbon nanotubes (MWCNT) films was electrodeposited on glassy carbon (GC) for the immobilization of acetylcholinesterase (AChE) as enzyme, in developing a biosensor for pesticides. The characterization and advantages of using the hybrid materials were examined by Fourier transform infrared spectroscopy (FTIR), field-emission gun scanning electron microscopy (FEG-SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The immobilization of AChE on the surface of the electrode was largely improved due to the unique synergistic effect of PANI and MWCNT. The developed GC/MWCNT/PANI/AChE biosensor was evaluated for determination of carbamate by square wave voltammetry (SWV) and chronoamperometry. SWV allowed carbaryl to be determined in a linear range between 9.9 and 49.6 nmol L À1, with a detection limit of 4.6 nmol L À1 and using chronoamperometry the analytical curve was linear in the concentration ranges from 9.9 to 49.6 mmol L À1 , with a detection limit of 1.4 mmol L À1 .

Synthesis and characterization of a novel non-enzymatic glucose biosensor based on polyaniline/zinc oxide/multi-walled carbon nanotube ternary nanocomposite

“…The graphitic peak at 2  = 26.4° can be attributed to the (002) reflection of MWCNT while it is relatively broad as compared to other carbon peaks. The lower intensity of this peak is indicative for a less number of adjacent graphite layers present in MWCNTs, and increased degree of nanotubes alignment [29,30]. Moreover, the peaks at 2θ values of 30.75, 36.20, 56.45, 62.72, and 66.25° are assigned to the (100), (101), (110), (103), and (200) crystal planes of ZnO nanoparticles, respectively.…”

Determination of carbamate pesticide in food using a biosensor based on reduced graphene oxide and acetylcholinesterase enzyme

“…Table 2 shows the comparison of different biosensors and their LOD values. Cesarino et al [46,47] used an electropolymerization polyaniline film for AChE immobilization on carbon nanotubes. An AChE-e-pGON/GCE was applied for the detection of carbaryl by Li et al [37].…”

Glass/PDMS hybrid microfluidic device integrating vertically aligned SWCNTs to ultrasensitive electrochemical determinations

“…4 CNT is a nanomaterial broadly used for the development of detectors and sensors. [16][17][18] Such compounds aggregate advantages which can improve the selectivity and sensitivity of the analytical method. Their main characteristics include: (i) high area/volume ratio (electrocatalytic activity); (ii) good thermal and chemical stabilities; (iii) conducting (ideal for nanoscale electrodes) 19 or semiconducting (excellent for nanoscale field effect transistors) 20 behavior; and (iv) high surface area.…”

“…Their main characteristics include: (i) high area/volume ratio (electrocatalytic activity); (ii) good thermal and chemical stabilities; (iii) conducting (ideal for nanoscale electrodes) 19 or semiconducting (excellent for nanoscale field effect transistors) 20 behavior; and (iv) high surface area. 18 The performance of electrochemical CE microchips with different electrode substrates covered by MWCNTs and SWCNTs was evaluated by Pumera et al…”