A borate-based peroxynitrite fluorescent probe and its application in fluorescence imaging of living cells

Reactive oxygen and nitrogen species are small reactive molecules derived from elements in the airoxygen and nitrogen. They are produced in biological systems to mediate fundamental aspects of cellular signaling but must be very tightly balanced to prevent indiscriminate damage to biological molecules. Small molecule probes can transmute the specific nature of each reactive oxygen and nitrogen species into an observable luminescent signal (or even an acoustic wave) to offer sensitive and selective imaging in living cells and whole animals. This review focuses specifically on small molecule probes for superoxide, hydrogen peroxide, hypochlorite, nitric oxide, and peroxynitrite that provide a luminescent or photoacoustic signal. Important background information on general photophysical phenomena, common probe designs, mechanisms, and imaging modalities will be provided, and then, probes for each analyte will be thoroughly evaluated. A discussion of the successes of the field will be presented, followed by recommendations for improvement and a future outlook of emerging trends. Our objectives are to provide an informative, useful, and thorough field guide to small molecule probes for reactive oxygen and nitrogen species as well as important context to compare the ecosystem of chemistries and molecular scaffolds that has manifested within the field.

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A borate-based peroxynitrite fluorescent probe and its application in fluorescence imaging of living cells

Abstract: The paper showed that 3b had high selectivity and sensitivity for ONOO−. The recognition was not disturbed by other active oxygen groups. Moreover, the probe 3b had low cytotoxicity and was successfully used to detect intracellular ONOO−.

Cited by 3 publications

References 46 publications

Multifunctional fluorescent probe for simultaneous revealing Cys and ONOO– dynamic correlation in the ferroptosis

Multifunctional fluorescent probe for simultaneous revealing Cys and ONOO– dynamic correlation in the ferroptosis

Rational design of a ratiometric fluorescent probe for selective visualizing peroxynitrite in mitochondria and evaluation of organ injury

Ex Tenebris Lux: Illuminating Reactive Oxygen and Nitrogen Species with Small Molecule Probes

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