A Brassinosteroid-Signaling Kinase Interacts with Multiple Receptor-Like Kinases in Arabidopsis

Xu, Peng; Xu, Shou-Ling; Li, Zi-Jing; Tang, Wenqiang; Burlingame, Alma L.; Wang, Wenfei

doi:10.1093/mp/sst105

Cited by 39 publications

(38 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It will be interesting to find out whether one RLK can interact with multiple RLCKs to regulate the same or different downstream responses. In fact, recent work showed that BRI1 interacts with and phosphorylates multiple members of the BSK subfamily and even distantly related RLCK subfamily members (38). This could be a general mechanism that integrates distinct processes and mediates cross-talks among signaling pathways in plants.…”

Section: Discussionmentioning

confidence: 99%

Receptor kinase complex transmits RALF peptide signal to inhibit root growth in Arabidopsis

Chen

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

148

181

View full text Add to dashboard Cite

A number of hormones work together to control plant cell growth. Rapid Alkalinization Factor 1 (RALF1), a plant-derived small regulatory peptide, inhibits cell elongation through suppression of rhizosphere acidification in plants. Although a receptor-like kinase, FERONIA (FER), has been shown to act as a receptor for RALF1, the signaling mechanism remains unknown. In this study, we identified a receptor-like cytoplasmic kinase (RPM1-induced protein kinase, RIPK), a plasma membrane-associated member of the RLCK-VII subfamily, that is recruited to the receptor complex through interacting with FER in response to RALF1. RALF1 triggers the phosphorylation of both FER and RIPK in a mutually dependent manner. Genetic analysis of the fer-4 and ripk mutants reveals RIPK, as well as FER, to be required for RALF1 response in roots. The RALF1-FER-RIPK interactions may thus represent a mechanism for peptide signaling in plants.plant hormone | feronia | phosphorylation

show abstract

Section: Discussionmentioning

confidence: 99%

Receptor kinase complex transmits RALF peptide signal to inhibit root growth in Arabidopsis

Chen

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

148

181

View full text Add to dashboard Cite

show abstract

“…The constitutive IOS1 and FLS2 association was further confirmed in Arabidopsis using transgenic lines overexpressing IOS1-GFP, while IOS1 was also found to be part of unstimulated and stimulated EFR complexes by in planta proteomics analysis of EFR-associated proteins . In addition to associating with PRRs, IOS1 interacts with BRASSINOSTEROID SIGNAL-KINASE3 in Arabidopsis (Xu et al, 2014b). In Arabidopsis, only a few proteins are known to be present in PRR complexes before elicitation by MAMPs.…”

Section: Ios1 Associates With Fls2 Efr and Bak1 In A Ligand-indepenmentioning

confidence: 99%

RETRACTED: The Arabidopsis Malectin-Like Leucine-Rich Repeat Receptor-Like Kinase IOS1 Associates with the Pattern Recognition Receptors FLS2 and EFR and Is Critical for Priming of Pattern-Triggered Immunity

et al. 2014

View full text Add to dashboard Cite

Plasma membrane-localized pattern recognition receptors such as FLAGELLIN SENSING2 (FLS2) and EF-TU RECEPTOR (EFR) recognize microbe-associated molecular patterns (MAMPs) to activate the first layer of plant immunity termed patterntriggered immunity (PTI). A reverse genetics approach with genes responsive to the priming agent b-aminobutyric acid (BABA) revealed IMPAIRED OOMYCETE SUSCEPTIBILITY1 (IOS1) as a critical PTI player. Arabidopsis thaliana ios1 mutants were hypersusceptible to Pseudomonas syringae bacteria. Accordingly, ios1 mutants demonstrated defective PTI responses, notably delayed upregulation of PTI marker genes, lower callose deposition, and mitogen-activated protein kinase activities upon bacterial infection or MAMP treatment. Moreover, Arabidopsis lines overexpressing IOS1 were more resistant to P. syringae and demonstrated a primed PTI response. In vitro pull-down, bimolecular fluorescence complementation, coimmunoprecipitation, and mass spectrometry analyses supported the existence of complexes between the membranelocalized IOS1 and FLS2 and EFR. IOS1 also associated with BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 (BAK1) in a ligand-independent manner and positively regulated FLS2/BAK1 complex formation upon MAMP treatment. Finally, ios1 mutants were defective in BABA-induced resistance and priming. This work reveals IOS1 as a regulatory protein of FLS2-and EFR-mediated signaling that primes PTI activation upon bacterial elicitation.

show abstract

“…Additional higher order RK mutants were then created by selecting other LRR-VIII-2 RK members that displayed high expression in the stigma: LIK1/BSR840, BSR440 and BSR430/NILR2 [39-41] (Figure S1E and Table S1-S3). The BSR440 and BSR430 genes are tandemly linked and so both genes were deleted using CRISPR to generate the bsr443Δ double mutant (Figure 2A).…”

Section: Resultsmentioning

confidence: 99%

Two groups ofArabidopsisreceptor kinases preferentially regulate the growth of intraspecies pollen tubes in the female reproductive tract

Lee

Goring

2020

Preprint

View full text Add to dashboard Cite

In flowering plants, continuous cell-cell communication between the compatible male pollen 15 grain/growing pollen tube and the female pistil is required for successful sexual reproduction. In 16Arabidopsis thaliana, the later stages of this dialogue are mediated by several peptide ligands 17 and receptor kinases that guide pollen tubes to the ovules for the release of sperm cells. Despite a 18 detailed understanding of these processes, a key gap remains on the nature of the regulators that 19 function at the earlier stages. Here, we report on two groups of A. thaliana receptor kinases, the 20 LRR-VIII-2 RK subclass and the SERKs, that function in the female reproductive tract to regulate 21 the compatible pollen grains and early pollen tube growth, both essential steps for the 22 downstream processes leading to fertilization. Multiple A. thaliana LRR-VIII-2 RK and SERK 23 knockout mutant combinations were created, and several phenotypes were observed such as 24 reduced wild-type pollen hydration and reduced pollen tube travel distances. As these mutant 25 pistils displayed a wild-type morphology, the observed altered responses of the wild-type pollen 26 are proposed to result from the loss of these receptor kinases leading to an impaired pollen-pistil 27 dialogue at these early stages. Furthermore, using pollen from related Brassicaceae species, we 28 also discovered that these receptor kinases are required in the female reproductive tract to 29 establish a reproductive barrier to interspecies pollen. Thus, we propose that the LRR-VIII-2 RKs 30 and the SERKs play a dual role in the preferential selection and promotion of intraspecies pollen 31 over interspecies pollen. 32 33 Key Words: Arabidopsis, receptor kinases, pollen, pistil, compatible intraspecies pollinations, 34 interspecies pollinations 35 is the identification of the key regulators that function in the earlier stages that take place in the 63 stigma/style immediately after pollination. Successful growth of pollen tubes through the A. 64 thaliana stigma/style is a prerequisite to these later stages as pollen tubes growing through the 65 stigma/style then acquire the competency to perceive ovular guidance cues [23]. So far, the only 66 4 putative early stage regulators that have been identified are the pollen-specific genes encoding 67 the Pollen Coat Protein-B (PCP-B) peptides which are required for pollen adhesion and 68 hydration [24]. 69Here, we report that members of two groups of RK genes, the LEUCINE RICH REPEAT (LRR)-70 VIII-2 RKs and the SOMATIC EMBRYOGENESIS RECEPTOR KINASES (SERKs), that are 71 required in the stigma and the female reproductive tract to promote compatible pollen grain 72 hydration and pollen tube growth and to establish a reproductive barrier against interspecies 73 pollen tube growth. The discovery of these RKs was enabled by employing an unconventional 74 approach of using an interspecies tissue-specific pseudokinase as a tool for discovery of 75 candidate RKs by using the assumption that pseudokinases typically associate ...

show abstract

A Brassinosteroid-Signaling Kinase Interacts with Multiple Receptor-Like Kinases in Arabidopsis

Cited by 39 publications

References 10 publications

Receptor kinase complex transmits RALF peptide signal to inhibit root growth in Arabidopsis

Receptor kinase complex transmits RALF peptide signal to inhibit root growth in Arabidopsis

RETRACTED: The Arabidopsis Malectin-Like Leucine-Rich Repeat Receptor-Like Kinase IOS1 Associates with the Pattern Recognition Receptors FLS2 and EFR and Is Critical for Priming of Pattern-Triggered Immunity

Two groups ofArabidopsisreceptor kinases preferentially regulate the growth of intraspecies pollen tubes in the female reproductive tract

Contact Info

Product

Resources

About