A Brief Review on the Mechanisms of miRNA Regulation

Cai, Yuanqing; Yu, Xiaomin; Hu, Songnian; Yu, Jun

doi:10.1016/s1672-0229(08)60044-3

Cited by 807 publications

(576 citation statements)

References 79 publications

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“…Because miRNAs regulate the levels of transcripts encoding for various proteins, miRNAs can affect diverse biological processes such as cell differentiation and development, proliferation and apoptosis, tumor genesis, immune response, metabolism, as well as host-parasite interactions in general (Cai et al, 2009). …”

Section: Mechanism For Mirna Functionsmentioning

confidence: 99%

Global analysis of microRNA species in the gall midgeMayetiola destructor

Du¹

2016

2016 International Congress of Entomology

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MicroRNA (miRNA) plays a role in nearly all the biological pathways and therefore may provide opportunities to develop new means to combat the Hessian fly, Mayetiola destructor, a destructive pest of wheat. This study presents a comprehensive analysis of miRNA species via deep-sequencing samples from Hessian fly second instar larvae, pupae and adults. A total of 921 unique miRNA species were identified from approximately 30 million sequence reads. Among the 921 miRNA species, only 22 were conserved among Hessian fly and other insect species, and 242 miRNA species were unique to Hessian fly, the remaining 657 share certain sequence similarities with pre-miRNA genes identified from various insect species. The abundance of the 921 miRNA species based on sequence reads varies greatly among the three analyzed stages, with 20 exclusively expressed in adults, two exclusively expressed in pupae and two exclusively expressed in second instar larvae. For miRNA species expressed in all stages, 722 were with reads lower than 10. The abundance of the remaining 199 miRNA species varied from zero to more than eight-fold differences among different stages. Putative miRNA-encoding genes were analyzed for each miRNA species. A single putative gene was identified for 594 miRNA species. Two putative genes were identified for 138 miRNA species. Three or more putative genes were identified for 86 miRNA species. The three largest families had 14, 23 and 34 putative coding genes, respectively. No gene was identified for the remaining 103 miRNA species. In addition, 1516 putative target genes were identified for 490 miRNA species based on known criteria for miRNA targets. The putative target genes are involved in a wide range of processes from nutrient metabolism to encoding effector proteins. Analysis of the expression patterns of miRNA and pre-miRNA for the miRNA family PC-5p-67443, which contains 91 genes, revealed that miRNA and pre-miRNA were expressed differently in different developmental stages, suggesting that different isogenes are regulated by different mechanisms, or pre-miRNAs had other functions in addition to as an intermediate for miRNA biogenesis. The large set of miRNA species identified here provides a foundation for future research on miRNA functions in Hessian fly and for comparative studies in other species. The differential expression patterns between a pre-miRNA and its encoded mature miRNA in a multigene family is an initial step toward understanding the functional significance of isogenes in dramatically expanded miRNA families.v

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Section: Mechanism For Mirna Functionsmentioning

confidence: 99%

Global analysis of microRNA species in the gall midgeMayetiola destructor

Du¹

2016

2016 International Congress of Entomology

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“…İkincisi ise Turkiye Klinikleri J Med Sci 2016;36 (1) endojen kaynaklı olup, genellikle 22 baz uzunluğunda olan ve translasyon aşamasını baskılayan miRNA'lardır. [9][10][11][12] RNAi mekanizmasının ilk adımı, hücre sitop lazmasında uzun çift zincirli RNA'nın Dicer enzimi tarafından 20-25 bazlık küçük RNA'lara parçalan-masıdır. Endoribonükleaz bir molekül olan Dicer, RNaz III (RNase III, Ribonuclease III) enzim ailesinin üyesidir.…”

Section: Rnai Mekani̇zmasi Ve Bi̇yogenezi̇unclassified

RNA Interference and Current Clinical Applications: Review

Bender¹,

Çelebi²,

Atalay³

2016

Turkiye Klinikleri J Med Sci

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“…MicroRNAs (miRNAs or miRs) are short, non-coding RNAs that can suppress gene expression though base pairing to the 3' untranslated regions (3'-UTR) of targets (3). An exact mapping of HCC oncogenesis and metastasis has not yet been established, but numerous previous studies have indicated that miRNAs serve key functions in HCC development (4)(5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-143 inhibits tumorigenesis in hepatocellular carcinoma by downregulating GATA6

Xue

Yin

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. MicroRNAs serve a critical role in human hepatocellular carcinoma (HCC) progression. However, the exact role of microRNA-143 (miR-143) in HCC remains unclear. The current study investigates the molecular mechanism of miR-143 in HCC. In cultured HepG2 and Bel7402 cell lines, miR-143 levels were raised by lentivirus transduction. This significantly inhibited HCC progression in terms of cell invasion and proliferation in both HepG2 and Bel7402 cell lines (P<0.05). MiR-143 also significantly decreased tumor implantation in vivo (P<0.05). Regulation of miR-143 on its direct target, GATA-binding factor 6 (GATA6), was investigated by multiple strategies, including dual-luciferase assay, quantitative polymerase chain reaction and western blot analysis. The results indicated that miR-143 was downregulated in both HCC cell lines and human tumors. GATA6 was identified as the downstream target of miR-143 in HCC, and overexpressing GATA6 was able to counter the tumor-suppressive effect of miR-143 on HCC in HepG2 and Bel7402 cells by significantly increasing proliferation and invasion rates (P<0.05). Therefore, a novel epigenetic pathway was identified in which miR-143 may suppress the malignancy of HCC by targeting GATA6.

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A Brief Review on the Mechanisms of miRNA Regulation

Cited by 807 publications

References 79 publications

Global analysis of microRNA species in the gall midgeMayetiola destructor

Global analysis of microRNA species in the gall midgeMayetiola destructor

RNA Interference and Current Clinical Applications: Review

MicroRNA-143 inhibits tumorigenesis in hepatocellular carcinoma by downregulating GATA6

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