A Broad-Spectrum Phage Endolysin (LysCP28) Able to Remove Biofilms and Inactivate Clostridium perfringens Strains

Lü, Rui; Liu, Banhong; Wu, Liting; Bao, Hongduo; Garcı́a, Pilar; Wang, Yongjuan; Zhou, Yan; Zhang, Hui

doi:10.3390/foods12020411

Cited by 19 publications

(11 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S. aureus is known for its multidrug-resistant nature, which is primarily due to its ability to form biofilms that are notoriously difficult to remove [ 37 ]. The antibiofilm activity of endolysin LysSYL, which is known for its rapid bactericidal activity against S. aureus , was assessed with a crystal violet assay, as described previously [ 38 ]. The results showed that 1×MIC of LysSYL (32 µg/mL) could significantly remove 24 h- and 72 h-old biofilms established by MRSA USA300, whereas 32×MIC of VAN had a less pronounced effect (32 µg/mL, Fig.…”

Section: Resultsmentioning

confidence: 99%

LysSYL: a broad-spectrum phage endolysin targeting Staphylococcus species and eradicating S. aureus biofilms

Liu,

Wei,

Wang

et al. 2024

Microb Cell Fact

View full text Add to dashboard Cite

Background Staphylococcus aureus and its single or mixed biofilm infections seriously threaten global public health. Phage therapy, which uses active phage particles or phage-derived endolysins, has emerged as a promising alternative strategy to antibiotic treatment. However, high-efficient phage therapeutic regimens have yet to be established. Results In this study, we used an enrichment procedure to isolate phages against methicillin-resistant S. aureus (MRSA) XN108. We characterized phage SYL, a new member of the Kayvirus genus, Herelleviridae family. The phage endolysin LysSYL was expressed. LysSYL demonstrated stability under various conditions and exhibited a broader range of efficacy against staphylococcal strains than its parent phage (100% vs. 41.7%). Moreover, dynamic live/dead bacterial observation demonstrated that LysSYL could completely lyse MRSA USA300 within 10 min. Scan and transmission electron microscopy revealed evident bacterial cell perforation and deformation. In addition, LysSYL displayed strong eradication activity against single- and mixed-species biofilms associated with S. aureus. It also had the ability to kill bacterial persisters, and proved highly effective in eliminating persistent S. aureus when combined with vancomycin. Furthermore, LysSYL protected BALB/c mice from lethal S. aureus infections. A single-dose treatment with 50 mg/kg of LysSYL resulted in a dramatic reduction in bacterial loads in the blood, liver, spleen, lungs, and kidneys of a peritonitis mouse model, which resulted in rescuing 100% of mice challenged with 108 colony forming units of S. aureus USA300. Conclusions Overall, the data provided in this study highlight the strong therapeutic potential of endolysin LysSYL in combating staphylococcal infections, including mono- and mixed-species biofilms related to S. aureus.

show abstract

Section: Resultsmentioning

confidence: 99%

LysSYL: a broad-spectrum phage endolysin targeting Staphylococcus species and eradicating S. aureus biofilms

Liu,

Wei,

Wang

et al. 2024

Microb Cell Fact

View full text Add to dashboard Cite

show abstract

“…NPs are particles with diameters ranging from 0 to 100 nm [22,23]. In recent years, there has been a considerable increase in research devoted to the antibacterial activity of NPs, with silver NPs (AgNPs) being one of the most investigated [22,24,25]. The synthesis of NPs is very often carried out by chemical or physical means, but unfortunately, these methods are of ecological concern, and therefore, more eco-friendly routes are needed [26,27].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of green-engineered silver nanoparticles using Cymbopogon citratus (lemongrass) and its antibacterial activity against clinical Pseudomonas aeruginosa

Arsene,

Davares,

Goriainov

et al. 2024

Int J One Health

View full text Add to dashboard Cite

Background and Aim: The use of bioengineered nanocomposites as antimicrobials has increased in recent years, but very few investigations have been conducted to test their effectiveness against Pseudomonas aeruginosa, a pathogen presenting public health risks that can impact both humans and animals. The aim of this study was to assess the antimicrobial potential of phytofabricated silver nanoparticles synthesized using lemongrass extract against clinical isolates of P. aeruginosa. Materials and Methods: The extraction of active compounds from the leaves of Cymbopogon citratus was performed using ethanol (80%) as a solvent, high-performance liquid chromatography-mass spectrometry was used to analyze the chemical composition of the extract, the synthesis of silver nanoparticles (AgNPs) was done using silver nitrate (AgNO3) as a precursor, and the antimicrobial and antibiofilm activity of the extract and the AgNPs phytofabricated was assessed against 10 clinical strains of P. aeruginosa. Results: Lemongrass extract was found to consist of the following main compounds: Caffeic acid (445.21 ± 32.77 μg/g), p-coumaric acid (393.32 ± 39.56 μg/g), chlorogenic acid (377.65 ± 4.26 μg/g), quinic acid (161.52 ± 17.62 μg/g), and quercetin-3-glucoside (151.35 ± 11.34 μg/g). AgNPs were successfully phytofabricated using 2.5 mM AgNO3. The ultraviolet (UV)-visible absorption spectra of the AgNPs showed a localized surface plasmon resonance at 464 nm with an absorbance of 0.32 A. The 50x hydrodynamic diameter was 50.29 nm with a surface area value of 120.10 m2/ cm3, and the volume mean diameter and Sauter mean diameter were 50.63 nm and 49.96 nm, respectively. Despite the compound found in lemongrass extract, no antimicrobial activity was observed with the extract, while AgNPs exhibited noteworthy dose-dependent antimicrobial activity with inhibition diameters up to 24 mm and minimum inhibitory concentration (MIC) and minimum bactericidal concentration ranging from 2 to 16 and 4–64 µg/mL, respectively. AgNPs also demonstrated significant antibiofilm activity by inhibiting biofilms up to 99% between MIC/2 and 2MIC. Conclusion: The present study suggests that lemongrass is a good candidate for the synthesis of AgNPs with good physicochemical characteristics and having a strong anti-pseudomonas activity. Further research is needed to assess the stability and safety of these AgNPs. Keywords: antibiofilm capacity, antimicrobial activity, green synthesis, lemongrass, Pseudomonas aeruginosa, silver nanoparticles.

show abstract

“…In general, antibiotic resistance has prompted a major mobilization in pursuing new antimicrobial compounds and alternative treatments [2]. Several matrices with physiological potentials, such as probiotics [6,7], medicinal plants [8][9][10], antimicrobial peptides [11,12], phages [13,14], and various nanoparticles (NPs) [3,15,16], are regularly suggested as the most credible alternatives to common antibiotics. In recent years, there has also been a considerable increase in research to study the antibacterial activity of metallic nanostructures and nanocomplexes, with silver NPs (AgNPs) being among the most investigated [3,[15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…Several matrices with physiological potentials, such as probiotics [6,7], medicinal plants [8][9][10], antimicrobial peptides [11,12], phages [13,14], and various nanoparticles (NPs) [3,15,16], are regularly suggested as the most credible alternatives to common antibiotics. In recent years, there has also been a considerable increase in research to study the antibacterial activity of metallic nanostructures and nanocomplexes, with silver NPs (AgNPs) being among the most investigated [3,[15][16][17]. The synthesis of NPs is very often performed by chemical or physical means, but unfortunately, these methods have ecological concerns, hence the need to use more eco-friendly routes [18,19].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of phytofabricated silver nanoparticles using Carica papaya L. against Gram-negative bacteria

Arsene,

Viktorovna,

Alla

et al. 2023

Vet World

View full text Add to dashboard Cite

Background and Aim: Antibiotic resistance, especially in Gram-negative bacteria, is a major public health risk affecting all industries requiring the use of antibiotics, including agriculture and animal breeding. This study aimed to use papaya extracts to synthesize silver nanoparticles (AgNPs) and evaluate their antimicrobial activity against various Gram-negative bacteria. Materials and Methods: Silver nanoparticles were synthesized from the aqueous extracts of papaya seed, root, and bark, with AgNO3 used as a reducing agent. The phytofabricated AgNPs were analyzed by ultraviolet–visible absorbance, X-ray diffraction (XRD), Fourier-transform infrared spectroscopy, and photon cross-correlation spectroscopy (PCCS). The disc-diffusion method was used to perform antibacterial analysis, and the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations were determined. We also investigated the antibiofilm activity of AgNPs and attempted to elucidate the potential mechanism of action on Escherichia coli ATCC 25922. Results: Phytofabrication of AgNPs was successful with papaya root (PR-AgNPs) and papaya seed (PS-AgNPs), but not with papaya bark. Silver nanoparticles using papaya root and PS-AgNPs were both cubic and showed maximum absorbances of 2.6 and 0.3 AUs at 411.6 and 416.8 nm wavelengths and average hydrodynamic diameters X50 of 59.46 ± 7.03 and 66.57 ± 8.89 nm, respectively. The Ag in both AgNPs was confirmed by X-ray fluorescence by a distinctive peak in the spectrum at the silver Ka line of 22.105 keV. Both AgNPs exhibited broad-spectrum antimicrobial and antibiofilm activity against all Gram-negative bacteria, and PR-AgNPs were slightly better than AgNPs-PS. The MIC ranged from 16 µg/mL–28 µg/mL and 16 µg/mL–64 µg/mL, respectively, for PS-AgNPs and PR-AgNPs. The elucidation of the mechanism of action revealed interference with E. coli ATCC 25922 growth kinetics and inhibition of HM+-ATPase proton pumps. Conclusion: Papaya seed and root extracts were efficient reducing agents for the biogenic synthesis of AgNPs, with noteworthy antibacterial and antibiofilm activities. Future studies should be conducted to identify the phytochemicals and the mechanism involved in AgNPs synthesis. Keywords: antibiotic resistance, biogenic synthesis, Carica papaya, Gram-negative, silver nanoparticles.

show abstract

A Broad-Spectrum Phage Endolysin (LysCP28) Able to Remove Biofilms and Inactivate Clostridium perfringens Strains

Cited by 19 publications

References 54 publications

LysSYL: a broad-spectrum phage endolysin targeting Staphylococcus species and eradicating S. aureus biofilms

LysSYL: a broad-spectrum phage endolysin targeting Staphylococcus species and eradicating S. aureus biofilms

Synthesis of green-engineered silver nanoparticles using Cymbopogon citratus (lemongrass) and its antibacterial activity against clinical Pseudomonas aeruginosa

Antimicrobial activity of phytofabricated silver nanoparticles using Carica papaya L. against Gram-negative bacteria

Contact Info

Product

Resources

About