A Building Block-Based Beam-Break (B
            <sup>5</sup>
            ) Locomotor Activity Monitoring System and Its Use in Circadian Biology Research

Tang, Qijun; Güler, Ali D.

doi:10.2144/btn-2022-0036

Cited by 2 publications

(4 citation statements)

References 10 publications

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“…For SF, mice were first acclimated to single housing for 7 day4s, followed by acclimation to infrared (IR) beam interruption chambers [Columbus Instruments, or custom built ( 114 )] for a minimum of 72 hours before starting the recording of locomotor activity. After at least 3 days of recording of baseline locomotor activity while the mice had ad libitum access to food, mice were fasted at lights off (ZT12) on day 0 of SF, along with a full cage change.…”

Section: Methodsmentioning

confidence: 99%

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Leptin receptor neurons in the dorsomedial hypothalamus input to the circadian feeding network

Tang,

Godschall,

Brennan

et al. 2023

Sci. Adv.

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Salient cues, such as the rising sun or availability of food, entrain biological clocks for behavioral adaptation. The mechanisms underlying entrainment to food availability remain elusive. Using single-nucleus RNA sequencing during scheduled feeding, we identified a dorsomedial hypothalamus leptin receptor–expressing (DMH LepR ) neuron population that up-regulates circadian entrainment genes and exhibits calcium activity before an anticipated meal. Exogenous leptin, silencing, or chemogenetic stimulation of DMH LepR neurons disrupts the development of molecular and behavioral food entrainment. Repetitive DMH LepR neuron activation leads to the partitioning of a secondary bout of circadian locomotor activity that is in phase with the stimulation and dependent on an intact suprachiasmatic nucleus (SCN). Last, we found a DMH LepR neuron subpopulation that projects to the SCN with the capacity to influence the phase of the circadian clock. This direct DMH LepR -SCN connection is well situated to integrate the metabolic and circadian systems, facilitating mealtime anticipation.

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Section: Methodsmentioning

confidence: 99%

“…Locomotor activity data were collected by wheel-equipped cages (Nalgene) or IR beam interruption chambers [Columbus Instruments, or custom built ( 114 )] in light-tight compartments under a 12:12-hour LD cycle. Fluorescent lights (~400 lux) were used for behavioral experiments.…”

Section: Methodsmentioning

confidence: 99%

Leptin receptor neurons in the dorsomedial hypothalamus input to the circadian feeding network

Tang,

Godschall,

Brennan

et al. 2023

Sci. Adv.

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show abstract

“…For scheduled feeding, mice were first acclimated to single housing for 7 days, followed by acclimation to IR beam interruption chambers (Columbus Instruments, or custom built 108 ) for a 34 minimum of 72 hours before starting the recording of locomotor activity. After at least 3 days of recording of baseline locomotor activity while the mice had ad libitum access to food, mice were fasted at lights off (ZT12) on day 0 of SF, along with a full cage change.…”

Section: Scheduled Feeding (Sf)mentioning

confidence: 99%

“…Locomotor activity data were collected by wheel-equipped cages (Nalgene), or IR beam interruption chambers (Columbus Instruments, or custom built 108 ) in light-tight compartments under a 12h:12h LD cycle. Fluorescent lights (~400 lux) were used for behavioral experiments.…”

Section: Circadian Behavioral Analysismentioning

confidence: 99%

A leptin-responsive hypothalamic circuit inputs to the circadian feeding network

Tang

Godschall

Brennan

et al. 2023

Preprint

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Salient cues, such as the rising sun or the availability of food, play a crucial role in entraining biological clocks, allowing for effective behavioral adaptation and ultimately, survival. While the light-dependent entrainment of the central circadian pacemaker (suprachiasmatic nucleus, SCN) is relatively well defined, the molecular and neural mechanisms underlying entrainment associated with food availability remains elusive. Using single nucleus RNA sequencing during scheduled feeding (SF), we identified a leptin receptor (LepR) expressing neuron population in the dorsomedial hypothalamus (DMH) that upregulates circadian entrainment genes and exhibits rhythmic calcium activity prior to an anticipated meal. We found that disrupting DMHLepR neuron activity had a profound impact on both molecular and behavioral food entrainment. Specifically, silencing DMHLepR neurons, mis-timed exogenous leptin administration, or mis-timed chemogenetic stimulation of these neurons all interfered with the development of food entrainment. In a state of energy abundance, repetitive activation of DMHLepR neurons led to the partitioning of a secondary bout of circadian locomotor activity that was in phase with the stimulation and dependent on an intact SCN. Lastly, we discovered that a subpopulation of DMHLepR neurons project to the SCN with the capacity to influence the phase of the circadian clock. This leptin regulated circuit serves as a point of integration between the metabolic and circadian systems, facilitating the anticipation of meal times.

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A Building Block-Based Beam-Break (B ⁵ ) Locomotor Activity Monitoring System and Its Use in Circadian Biology Research

Cited by 2 publications

References 10 publications

Leptin receptor neurons in the dorsomedial hypothalamus input to the circadian feeding network

Leptin receptor neurons in the dorsomedial hypothalamus input to the circadian feeding network

A leptin-responsive hypothalamic circuit inputs to the circadian feeding network

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A Building Block-Based Beam-Break (B 5 ) Locomotor Activity Monitoring System and Its Use in Circadian Biology Research

Cited by 2 publications

References 10 publications

Leptin receptor neurons in the dorsomedial hypothalamus input to the circadian feeding network

Leptin receptor neurons in the dorsomedial hypothalamus input to the circadian feeding network

A leptin-responsive hypothalamic circuit inputs to the circadian feeding network

Contact Info

Product

Resources

About

A Building Block-Based Beam-Break (B ⁵ ) Locomotor Activity Monitoring System and Its Use in Circadian Biology Research