A C-type lectin TcCTL1 is required for embryogenesis in Tribolium castaneum

Zhang, Yonglei; Zhang, Ping; Yu, Runnan; Li, Bin

doi:10.1016/j.dci.2022.104560

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…Quantitative real‐time PCR (qRT‐PCR) was performed to determine all the gene expression levels using SYBR Green Master (Vazyme). Ribosomal protein S3 (rps3) was used as a reference for data standardization, and relative expression levels of the genes were calculated according to the 2 −∆∆ CT method 20,21 …”

Section: Methodsmentioning

confidence: 99%

“…Ribosomal protein S3 (rps3) was used as a reference for data standardization, and relative expression levels of the genes were calculated according to the 2 −ΔΔCT method. 20,21 2.6 RNAi and behavioral analysis Double-stranded RNA synthesis, injection, and behavior analysis were performed in accordance with the procedure described in a previous study. 22 Briefly, PCR products produced by dsTcGclc primers, including gene-specific sequences, and T7 polymerase promoter were purified using a gel purification kit (product code: DC301; Vazyme).…”

Section: Quantitative Real-time Pcr Analysismentioning

confidence: 99%

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The glutathione biosynthesis is involved in metamorphosis, antioxidant function, and insecticide resistance in Tribolium castaneum

Kim,

Gao,

et al. 2024

Pest Management Science

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BACKGROUNDReduced glutathione (GSH) synthesis is vital for redox homeostasis, cell‐cycle regulation and apoptosis, and immune function. The glutamate–cysteine ligase catalytic subunit (Gclc) is the first and rate‐limiting enzyme in GSH synthesis, suggesting the potential use of Gclc as a pesticide target. However, the functional characterization of Gclc, especially its contribution in metamorphosis, antioxidant status and insecticide resistance, is unclear in Tribolium castaneum.RESULTSIn this study, we identified and cloned Gclc from T. castaneum (TcGclc) and found that its expression began to increase significantly from the late larvae (LL) stage (3.491 ± 0.490‐fold). Furthermore, RNA interference‐mediated knockdown of TcGclc resulted in three types of aberration (100% total aberration rate) caused by the downregulation of genes related to the 20‐hydroxyecdysone (20E) pathway. This deficiency was partially rescued by exogenous 20E treatment (53.1% ± 3.2%), but not by antioxidant. Moreover, in the TcGclc knockdown group, GSH content was decreased to 62.3%, and total antioxidant capacity, glutathione peroxidase and total superoxide dismutase activities were reduced by 14.6%, 83.6%, and 82.3%, respectively. In addition, treatment with different insecticides upregulated expression of TcGclc significantly compared with a control group during the late larval stage (P < 0.01).CONCLUSIONOur results indicate that TcGclc has an extensive role in metamorphosis, antioxidant function and insecticide resistance in T. castaneum, thereby expanding our understanding of GSH functions and providing a scientific basis for pest control. © 2024 Society of Chemical Industry.

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Section: Methodsmentioning

confidence: 99%

Section: Quantitative Real-time Pcr Analysismentioning

confidence: 99%

The glutathione biosynthesis is involved in metamorphosis, antioxidant function, and insecticide resistance in Tribolium castaneum

Kim,

Gao,

et al. 2024

Pest Management Science

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C‐Type Lectin S Group TcCTL4 Participates in the Immunity of Tribolium castaneum

Zhang,

Ai,

Zhang

et al. 2024

Arch Insect Biochem Physiol

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C‐type lectin S (CTL‐S) plays a crucial role in pathogen recognition and the activation of immune response. In comparison, the proportion of CTL‐S was relatively high in insects, but the study was much smaller than the proportion observed. In this study, we cloned and characterized one CTL‐S, TcCTL4, from Tribolium castaneum. Our analysis revealed that TcCTL4 was highly expressed during the early pupal stage, with expression levels exhibiting a tendency to change with developmental stages. Additionally, tissue expression analysis showed a high expression of TcCTL4 in the central nervous system (CNS). Moreover, we observed a significant increase in TcCTL4 transcripts after bacterial challenge. The RNA interference (RNAi) of TcCTL4 before bacterial treatment resulted in a significant reduction in the transcripts of immune factors (IFs) and antimicrobial peptides (AMPs), indicating that TcCTL4 may regulate AMP expression through the activation of the immune signaling pathway. Furthermore, our investigation revealed that the recombinant protein TcCTL4 (rTcCTL4) not only recognized bacteria but also agglutinated bacteria in a Ca2+‐dependent manner. Enzyme activity analysis suggested that rTcCTL4 could enhance phenoloxidase activity, implying its potential involvement in the prophenoloxidase activation pathway. In conclusion, these results indicate that TcCTL4 is involved in the immunity of T. castaneum, providing valuable insights into insect CTLs.

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Minus-C odorant binding protein TcasOBP7G contributes to reproduction and defense against phytochemical in the red flour beetle, Tribolium castaneum

Gao,

Zhang,

Zhou

et al. 2023

Journal of Asia-Pacific Entomology

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A C-type lectin TcCTL1 is required for embryogenesis in Tribolium castaneum

Cited by 4 publications

References 29 publications

The glutathione biosynthesis is involved in metamorphosis, antioxidant function, and insecticide resistance in Tribolium castaneum

The glutathione biosynthesis is involved in metamorphosis, antioxidant function, and insecticide resistance in Tribolium castaneum

C‐Type Lectin S Group TcCTL4 Participates in the Immunity of Tribolium castaneum

Minus-C odorant binding protein TcasOBP7G contributes to reproduction and defense against phytochemical in the red flour beetle, Tribolium castaneum

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