A calmodulin antagonist protects in vitro raspberries against disturbed photosynthesis caused by constant light and cytokinin

Murvanidze, Nino; Ameye, Maarten; Geelen, Danny; Werbrouck, Stefaan

doi:10.1007/s11240-021-02165-5

Cited by 2 publications

(1 citation statement)

References 41 publications

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“…Although cytokinins might be crucial for driving or improving in vitro responses, the shoots may show physiological and anatomical disorders induced by them (Dobránszki and Mendler-Drienyovszki 2014; Rosa et al, 2018). Under exogenous cytokinin exposure, plants may present with alterations in shoot length (visibly shorter shoots), decreased chlorophyll content, chlorotic leaves, shoot-tip necrosis, and low performance of the photosynthetic apparatus (Gentile et Murvanidze et al 2022). Morphological disorders induced in vitro by synthetic cytokinins, such as shoot-tip necrosis, have been previously reported in woody plant species, including Quercus (Vieitez et al 1985(Vieitez et al , 1997(Vieitez et al , 2009Martínez et al 2017;Surakshitha et al 2019).…”

Section: Introductionmentioning

confidence: 99%

6-Benzylaminopurine and kinetin modulations during in vitro propagation of Quercus robur (L.): an assessment of anatomical, biochemical, and physiological profiling of shoots

Martins

Wawrzyniak

Ley-López

et al. 2022

Plant Cell Tiss Organ Cult

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For many woody species, such as Quercus robur, cytokinins in the culture medium are required to maintain in vitro plant material. Among synthetic cytokinins, 6-benzylaminopurine (BAP) and kinetin (KIN) are the most frequently used. In addition to inducing shoots, cytokinins can cause morphophysiological disorders. Therefore, we aimed to investigate the anatomical, biochemical, and physiological alterations and pro les of Q. robur shoots exposed to two cytokinins, applied alone and in combination. Shoots previously established in vitro were transferred to WPM culture media supplemented with BAP at concentrations of 0, 1.25, and 3.50 µM combined with KIN at concentrations of 0, 0.62, and 1.25 µM totaling 9 treatments. Anatomical, physiological, and biochemical analyses were performed after 40 d of culture. BAP induced the formation of new buds with anatomically underdeveloped leaves; induced shoot-tip necrosis, which is considered a response to the ine cient transport of water and nutrients; reduced the thickness of the cell walls of phloem bers; and decreased the content of phenolic compounds and photosynthetic pigments. These responses were less pronounced with co-exposure to KIN. In contrast, KIN alone stimulated a larger area of secondary xylem and more ligni ed cell walls. BAP can induce shoots with underdeveloped anatomical and biochemical characteristics. Shoots that grew with KIN alone had stem and leaf anatomical characteristics, indicating greater commitment to cellular differentiation than proliferation. When both cytokinins are combined, KIN can partially mitigate the deleterious effects of BAP on in vitro growth.

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Section: Introductionmentioning

confidence: 99%

6-Benzylaminopurine and kinetin modulations during in vitro propagation of Quercus robur (L.): an assessment of anatomical, biochemical, and physiological profiling of shoots

Martins

Wawrzyniak

Ley-López

et al. 2022

Plant Cell Tiss Organ Cult

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Regeneración de explantes de hojas de cinco genotipos de frambuesa

Rosas-Rojas,

Ochoa-Alejo,

Rocha-Granados

2023

Remexca

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La eficiencia en la regeneración de frambuesa, a partir de esplantes de hojas, se ve limitada debido a diversos factores, entre que los que destacan la edad del explante y el genotipo. El objetivo de esta investigación fue determinar el efecto de los reguladores de crecimiento sobre la oxidación y la regeneración in vitro a partir de explantes de hojas de cinco genotipos de frambuesa, el año del estudio fue en 2021. Se probaron dosis y combinaciones de auxinas y citocininas para inducir organogénesis directa en explantes foliares de los genotipos de frambuesa; ‘C-6’, ‘Joan J.’, ‘A-1’, ‘UM-702’ y ‘Heritage’. Los resultados mostraron que el regulador bencilaminopurina (BAP) disminuyó la oxidación en los genotipos ‘C-6’, ‘Joan J.’, ‘A-1’ y ‘Heritage’ en 36, 48, 60 y 68% respectivamente, los que se suplementaron con cinetina tuvieron una reducción de la oxidación en el genotipo ‘C-6’ (56%), cuando se adicionó tidiazuron (TDZ) la oxidación disminuyó en los genotipos evaluados en 72, 64, 72, 84 y 68% respectivamente. La mayor regeneración (número de brotes/explate) fue con BAP (0.5 mg L-1) y TDZ (0.2 mg L-1) + ácidoindolbutírico (AIB) (0.1 mg L-1) para el genotipo ‘C-6’, y TDZ (0.2 mg L-1) + AIB (0.1 mg L-1) para ‘Joan J.’ y ‘Heritage’. En ‘A-1’y ‘UMC-702’ se sugiere el uso de TDZ (0.2 mg L-1) solo. Se concluye que el uso de reguladores de crecimiento, solos o combinanados, disminuyen la oxidación en los explantes de hojas, y aumentan la sobrevivencia y regeneración de brotes en todos los genotipos evaluados.

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A calmodulin antagonist protects in vitro raspberries against disturbed photosynthesis caused by constant light and cytokinin

Cited by 2 publications

References 41 publications

6-Benzylaminopurine and kinetin modulations during in vitro propagation of Quercus robur (L.): an assessment of anatomical, biochemical, and physiological profiling of shoots

6-Benzylaminopurine and kinetin modulations during in vitro propagation of Quercus robur (L.): an assessment of anatomical, biochemical, and physiological profiling of shoots

Regeneración de explantes de hojas de cinco genotipos de frambuesa

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