A canine linkage map: 39 linkage groups

Linkage of the canine-derived microsatellites in the red fox (Vulpes vulpes) and arctic fox (Alopex lagopus) genomes

“…The corresponding estimates for the arctic fox were 0.20, 0.0 and 0.11. Also, in the canine marker gene map the microsatellities CPH8 and 2206 are tightly linked on CFA19 (M ellersh et al 2000; L ingaas et al 2001). On the fox genome these loci seem to be located at a larger distance apart.…”

“…The LGA1 includes two loci which appear on two chromosomes in the dog genome:6 (CPH3) and 23 (CPH6) (M ellersh et al 2000; L ingaas et al 2001). The estimated female, male and sex‐average recombination fractions were 0.24, 0.0 and 0.14, respectively.…”

Microsatellite polymorphism and genetic distances between the dog, red fox and arctic fox

“…The dog marker genome map has been extensively developed during the recent years. At present almost 2000 microsatellite (MS) markers have been described and many of them have been assigned to linkage groups or/and specific chromosomes ( Breen et al 2001; Lingaas et al 2001; Mellersh et al 2000). Canine‐derived primers for MS loci are useful in studies of the arctic fox ( Alopex lagopus ) and the red fox ( Vulpes fulvus ) genomes ( Fredholm and Wintero 1995; Zajac et al 2000), and are a potentially rich resource for the fox genome mapping, population analysis and parentage control.…”

Erratum

“…Source/description: Canis familiaris DNA was digested with Sau3AI and fragments ranging 700-1000 bp in length were ligated into the BamHI site of a pre-digested and de-phosphorylated pUC18 vector (Amersham Pharmacia Biotech, Amersham, UK) and transformed into Escherichia coli SURE Ò cells (Stratagene, La Jolla, CA, USA). A total of 1200 recombinant clones were pooled in groups of 10 and the pools were spotted onto a nylon membrane, screened with digoxigeninlabelled probes (TG) 10 and (AAAT) 7 , and the positive pools were screened individually to isolate the final positive clones. Ten positive clones were sequenced in an ABI-310 sequencer (Applied Biosystems, Foster City, CA, USA) from which six true-positive clones were obtained (60%) and are detailed in Table 1.…”

“…Markers were assigned to previously described linkage groups 7 and anchored into chromosomes by using the ÔtwopointÕ option, and arranged with the ÔbuildÕ option. Linkage groups and chromosomal locations of the new loci are shown in Table 1 and depicted in Fig.…”