A Capillary Electrophoresis Method Based on Molecularly Imprinted Solid-Phase Extraction for Selective and Sensitive Detection of Histamine in Foods

Abstract

In this study, a sensitive capillary electrophoresis (CE) method based on molecularly imprinted solid-phase extraction (MISPE) was proposed to determine histamine in foods. A molecularly imprinted polymer (MIP) synthesized by bulk polymerization was used as the MISPE adsorbent for the selective extraction of histamine. Under the optimal conditions, the MISPE-CE method possessed good linearity for histamine detection in the concentration range of 0.1–100.0 μg/L. The limit of detection and limit of quantification of the method were calculated to be 0.087 μg/L and 0.29 μg/L, respectively. The histamine in spiked rice vinegar and liquor samples were detected by the developed method with recoveries of 92.63–111.00%. The histamine contents in fish, prawn, pork, chicken breast and soy sauce samples were determined using the developed method and a high-performance liquid chromatography method, with no significant difference found between the two methods.

Capillary electromigration methods for food analysis and Foodomics: Advances and applications in the period March 2021 to March 2023

“…In this work, the molecular imprinted SPE obtained by bulk polymerization with methacrylic acid, 2,2′‐azobis (2‐methylproppionitrile) and ethylene glycol dimethacrylate allowed the recovery of higher histamine concentrations (around 100%) from rice vinegar and liquors than employing C18 cartridges. This method provided a LOD of 0.087 µg/L in the subsequent CE analysis [32].…”

“…In particular, the challenge in these last years has been the development of new preconcentration steps for a more selective and sensitive detection of AAs and BAs by CE. In this sense, for the determination of histamine, a combination of solid-phase extraction (SPE) with a molecular imprinted polymer with specific binding sites to histamine was developed as sample pretreatment to improve the LOD of this specific BA [32]. In this work, the molecular imprinted SPE obtained by bulk polymerization with methacrylic acid, 2,2′-azobis (2-methylproppionitrile) and ethylene glycol dimethacrylate allowed the recovery of higher histamine concentrations (around 100%) from rice vinegar and liquors than employing C18 cartridges.…”

Inspecting Histamine Isolated from Fish through a Highly Selective Molecularly Imprinted Electrochemical Sensor Approach

“…Wang et al 40 Ahmed et al 25 Mahnashi et al 41 Fan et al 42 Peng et al 43 The determination of histamine using MIP/SPE/PU/ LiClO 4 using the voltammetric approach presents a satisfactory outcome. Generally, voltammetry relies on the development of current signal and charge upon histamine as the analyte and MIP as the receptor binder.…”

Sensitivity Enhancement for Separation-Based Analytical Techniques Utilizing Solid-Phase Enrichment Approaches and Analyte Derivatization for Trace Analysis in Various Matrices

“…Cantú et al reported an offline SPME technique for analyzing anticonvulsants and tricyclic antidepressants in human plasma for TDM purposes by HPLC-UV [44]. For measuring various medicines in several matrices using offline SPE, different separation-based analytical approaches have been proposed [45][46][47][48][49][50][51][52][53][54][55][56][57][58]. Table 1 lists the LODs, LOQs, sample matrices, and offline extraction and separation processes as well as the detection systems used for the analysis of various pharmaceutics.…”