A capillary zone electrophoresis (CZE) method for the determination of cyclizine hydrochloride in tablets and suppositories

Mohammadi, Ali; Kanfer, Isadore; Walker, Roderick B.

doi:10.1016/j.jpba.2004.01.011

“…The authors applied the method on tablets, suppositories and on raw material of the drug stored in 10% v/v hydrogen peroxide solution to follow drug degradation [70].…”

Section: Central Nervous System Drugsmentioning

confidence: 99%

Impurity analysis of pharmaceuticals using capillary electromigration methods

Jouyban

¹

,

Kenndler

²

2008

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This review deals with the determination of impurities in pharmaceuticals by electromigration methods in the capillary format. These separation methods are either based on the different effective mobility of the charged analytes (as in zone electrophoresis and isotachophoresis) or include hybrid methods such as micellar electrokinetic chromatography, microemulsion electrokinetic chromatography and electrochromatography. The pharmaceutically active compounds under consideration belong to chemotherapeutic agents, central nervous system drugs, histamine receptor drugs, cardiovascular drugs, anticancer drugs, anti-inflammatory drugs and some other drugs. The review discusses about 150 publications from the period between 1980 and 2007 with special emphasis on the recent trends and gives details about the experimental conditions applied for analyses and the obtained analytical performance parameters.

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“…A literature review revealed that several approaches to the determination of CYZ have been identified, either in the formulation of raw materials or pharmaceuticals, either alone or in conjunction with other medications. These recorded analytical approaches include non-aqueous titration [1], spectrophotometric [9][10][11], TLC-densitometric [12][13][14], HPLC [15][16][17][18][19][20], capillary zone electrophoretic [21], and potentiometric [22][23][24][25] methods. Neither in its pharmaceutical formulation nor in biological fluids, have no spectrofluorimetric methods for determining CYZ been published.…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric Approach for Quantification of Cyclizine in the Presence of its Toxic Impurities in Human Plasma; in silico Study and ADMET Calculations

Fares

¹

,

Abdelwahab

²

,

El-Sayed

³

et al. 2022

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Cyclizine (CYZ); an antiemetic compound; is widely misused for its euphoric or hallucinatory effects, either by oral or intravenous routes. The concomitant abuse of CYZ among addicted adolescents contributes to neuromuscular disorders that are life-threatening. Consequently, with the company of 1-Methylpiperazine (MPZ) and diphenylmethanol (DPM, Benzhydrol) as pharmacopoeia-reported CYZ impurities, a novel spectrofluorimetric assay for the detection of CYZ, has been established either in human plasma samples or in its parenteral formulation. The native fluorescence of CYZ has been investigated under various conditions. Different parameters affecting relative fluorescence intensity of CYZ including diluting solvent, surfactant, plasma protein solvent, and pH were studied and optimized. The linearity obtained between the fluorescence intensity at emission wavelength 350 nm after excitation at 244 nm and the corresponding CYZ concentrations was in the range 10–1000 ng/mL for measurement of CYZ either in pure form or in human plasma samples, with a appropriate correlation coefficient (r = 0.9999) and 3.10 ng/mL as the limit of detection and 9.41 ng/mL as the limit of quantitation. The suggested procedure was created and validated in accordance with ICH guidelines for quantification of CYZ either in its pure form or its dosage form, and FDA guidelines for the assay of CYZ in human plasma. Finally, in silico study and ADMET predictions were conducted for the studied drug impurities to estimate their pharmacokinetic behaviors. The results showed that both CYZ impurities have higher cellular permeability and maximum tolerated doses, DPM has higher BBB and CNS permeability than MPZ, while MPZ exceeds DPM in total clearance and volume of distribution. Graphic Abstract

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“…A literature review revealed that several approaches to the determination of CYZ have been identi ed, either in the formulation of raw materials or pharmaceuticals, either alone or in conjunction with other medications. These recorded analytical approaches include non-aqueous titration [1], spectrophotometric [9][10][11], TLC-densitometric [12][13][14], HPLC [15][16][17][18][19][20], capillary zone electrophoretic [21], and potentiometric [22][23][24][25] methods. Neither in its pharmaceutical formulation nor in biological uids, have no spectro uorimetric methods for determining CYZ been published.…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric Approach For Quantification of Cyclizine In The Presence of Its Toxic Impurities In Human Plasma; In Silico Study And ADMET Calculations

Fares

¹

,

Abdelwahab

²

,

Sayed

³

et al. 2021

Preprint

0

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Cyclizine (CYZ); an antiemetic compound; is widely misused for its euphoric or hallucinatory effects, either by oral or intravenous routes. The concomitant abuse of CYZ among addicted adolescents contributes to neuromuscular disorders that are life-threatening. Consequently, with the company of 1-Methylpiperazine (MPZ) and diphenylmethanol (DPM, Benzhydrol) as pharmacopoeia-reported CYZ impurities, a novel spectrofluorimetric assay for the detection of CYZ, has been established either in human plasma samples or in its parenteral formulation. The native fluorescence of CYZ has been investigated under various conditions. Different parameters affecting relative fluorescence intensity of CYZ including diluting solvent, surfactant, plasma protein solvent, and pH were studied and optimized. The linearity obtained between the fluorescence intensity at emission wavelength 350 nm after excitation at 244 nm and the corresponding CYZ concentrations was in the range 10–1000 ng/mL for measurement of CYZ either in pure form or in human plasma samples, with a appropriate correlation coefficient (r = 0.9999) and 3.10 ng/mL as the limit of detection and 9.41 ng/mL as the limit of quantitation. The suggested procedure was created and validated in accordance with ICH guidelines for quantification of CYZ either in its pure form or its dosage form, and FDA guidelines for the assay of CYZ in human plasma. Finally, in silico study and ADMET predictions were conducted for the studied drug impurities to estimate their pharmacokinetic behaviors. The results showed that both CYZ impurities have higher cellular permeability and maximum tolerated doses, DPM has higher BBB and CNS permeability than MPZ, while MPZ exceeds DPM in total clearance and volume of distribution.

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A capillary zone electrophoresis (CZE) method for the determination of cyclizine hydrochloride in tablets and suppositories

Cited by 15 publications

References 14 publications

Impurity analysis of pharmaceuticals using capillary electromigration methods

Impurity analysis of pharmaceuticals using capillary electromigration methods

Spectrofluorimetric Approach for Quantification of Cyclizine in the Presence of its Toxic Impurities in Human Plasma; in silico Study and ADMET Calculations

Spectrofluorimetric Approach For Quantification of Cyclizine In The Presence of Its Toxic Impurities In Human Plasma; In Silico Study And ADMET Calculations

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