A capillary zone electrophoresis method for determining N‐acetylneuraminic acid in glycoproteins and blood sera

Strousopoulou, K.; Militsopoulou, Maria; Stagiannis, Konstantinos D.; Lamari, Fotini N.; Karamanos, Nikos K.

doi:10.1002/bmc.143.abs

Cited by 6 publications

(8 citation statements)

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“…An AMAC-derivatization method was also successfully applied in the analysis of sialic acid in human urinary trypsin inhibitor (hu-UTI), bovine a1-acid glycoprotein (a1-AGP), and recombinant human erythropoietin (rhu-EPO) [137]. Other derivatization methods were also studied demonstrated to be effective derivatization reagents, such as 7-aminonaphthalene-1,3-disulfonic acid (ANDSA) [138] and per-O-benzoylation [139]. A recent report showed that with an argon laser LIF detector a high sensitivity (nM or amol) could be obtained following the derivatization procedure for sugars converted to N-methylglycamines in the presence of dimethylamine-borane complex at pH 4.5 and 407C [140].…”

Section: Sialic Acid and Acid Glycoproteinmentioning

confidence: 99%

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Liu

et al. 2004

Electrophoresis

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Investigation of effective biomarkers for cancers is currently a popular area of study in clinical and cancer researches, because it can potentially lead to pre-cancer screening or pre-cancer diagnosis and may provide useful information on cancer type and the disease's stage of progression. More and more biochemical or chemical fluid components of the human body such as urine, blood, and cerebrospinal fluid have been considered to contain biomarkers, which are useful in cancer researches, pre-cancer diagnosis, and cancer follow-ups during or after cancer treatment. Several modern analytical techniques, such as gas chromatography (GC), high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and other separation techniques as well as hyphenated techniques, have been extensively used in study of cancer biomarkers. Among these techniques, CE is considered to be a highly efficient and practical analytical technique because of the small sample volume requirement and its wide separation versatility, ranging from small inorganic molecules to large biomolecules. This review discusses the latest developments involving biomarkers and their analysis by CE, including a discussion of instrumental conditions, method developments, and data analysis.

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Section: Sialic Acid and Acid Glycoproteinmentioning

confidence: 99%

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Liu

et al. 2004

Electrophoresis

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“…Around the turn of the century, also capillary electrophoresis (CE) was introduced for the analysis of derivatized and nonderivatized Neu5Ac and Neu5Gc. [364][365][366][367][368][369]…”

Section: Chromatographic Techniquesmentioning

confidence: 99%

Exploration of the Sialic Acid World

Schauer

Kamerling

2018

Advances in Carbohydrate Chemistry and Biochemistry

261

225

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The Sialic Acid World Table 1 Survey of Reported Structures of Naturally Occurring Members of the Sialic Acid Family-cont'd Name Abbreviation References5-N-Acetyl-8-O-acetyl-9-Olactyl-neuraminic acid Neu5,8Ac 2 9Lt 45 5-N-Acetyl-8-O-methylneuraminic acid Neu5Ac8Me 6,19,25,33,46 5-N-Acetyl-4-O-acetyl-8-Omethyl-neuraminic acid Neu4,5Ac 2 8Me 19 5-N-Acetyl-9-O-acetyl-8-Omethyl-neuraminic acid Neu5,9Ac 2 8Me 6,19,33,46 5-N-Acetyl-9-O-methylneuraminic acid Neu5Ac9Me 47 5-N-Acetyl-4-O-sulfoneuraminic acid Neu5Ac4S 48 5-N-Acetyl-8-O-sulfoneuraminic acid Neu5Ac8S 19,32,49,50 5-N-Acetyl-4-O-acetyl-8-Osulfo-neuraminic acid Neu4,5Ac 2 8S 19 5-N-Acetyl-9-O-phosphoneuraminic acid f,g Neu5Ac9P 51 5-N-Acetyl-2-deoxy-2,3didehydro-neuraminic acid g,h Neu2en5Ac 6,30,33,43,52-54 5-N-Acetyl-9-O-acetyl-2deoxy-2,3-didehydroneuraminic acid g Neu2en5,9Ac 2 55,56 5-N-Acetyl-2-deoxy-2,3didehydro-9-O-lactylneuraminic acid g Neu2en5Ac9Lt 55,565-N-Acetyl-2,7-anhydroneuraminic acid g,i

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“…Also, there are a number of Neu5Ac residues at the terminal ends of the sugar chains (reported as 10 -15 mol/mol), and the determination of their content has potential for the diagnosis of various diseases. 18,19,42 While the hydrolysate of AGP was analyzed directly by normal CZE, the peak of the hydrolysate completely overlapped with the peak of Neu5Ac (Fig. 4a).…”

Section: Determination Of Neu5ac Content In α1-acid Glycoprotein Samplementioning

confidence: 99%

“…[18][19][20][21] In these chromatographic and electrophoretic methods, chemical derivatization techniques using a chromophore with strong absorption in the UV/Vis spectral region were often employed to give detection selectivity, although these were not related to the separation selectivity. Recently, fluorescence detection following derivatization with a fluorophore and mass spectrometric detection in conjunction with LC and CE were also reported for higher sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Affinity Capillary Electrophoresis for Selective Control of Electrophoretic Mobility of Sialic Acid Using Lanthanide-Hexadentate Macrocyclic Polyazacarboxylate Complexes

et al. 2015

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A capillary zone electrophoresis method for determining N‐acetylneuraminic acid in glycoproteins and blood sera

Cited by 6 publications

References 0 publications

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Exploration of the Sialic Acid World

Affinity Capillary Electrophoresis for Selective Control of Electrophoretic Mobility of Sialic Acid Using Lanthanide-Hexadentate Macrocyclic Polyazacarboxylate Complexes

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