A carbon dot and gold nanoparticle-based fluorometric immunoassay for 8-hydroxy-2′-deoxyguanosine in oxidatively damaged DNA

Hu, Wei; Chen, Tian; Zhang, Yu; Ye, Weiwei

doi:10.1007/s00604-019-3392-y

Cited by 7 publications

(6 citation statements)

References 42 publications

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“…In comparison with previous methods including quartz crystal microbalance (QCM), electrochemical sensors, and fluorescence aptasensors for 8-OHdG detection, the study presented here has the advantages of low LOD, wide detection range, and excellent sensitivity. The reaction time for 8-OHdG sensing in the study is longer than some reported studies [38,43]. It allows 8-OHdG to pass through the membrane pores and react with the antibody in the nanopores.…”

Section: Detection Of 8-ohdg By the Fret Biosensormentioning

confidence: 82%

“…CDs were synthesized with citric acid and EDTA by one-step hydrothermal treatment [ 38 ]. Citric acid (0.22 g) was dissolved in deionized water (DI water, 10 mL).…”

Section: Methodsmentioning

confidence: 99%

“…These advantageous merits make CDs attractive candidates in the areas of sensing and catalysis applications [ 32 , 33 , 34 , 35 , 36 , 37 ]. CDs together with gold nanoparticles have been used to develop a FRET assay method for detecting DNA containing oxidatively damaged product 8-OHdG (DNA-8-OHdG) [ 38 ]. CDs and gold nanoparticles acted as the donor and acceptor pair.…”

Section: Introductionmentioning

confidence: 99%

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A Sensitive FRET Biosensor Based on Carbon Dots-Modified Nanoporous Membrane for 8-hydroxy-2′-Deoxyguanosine (8-OHdG) Detection with Au@ZIF-8 Nanoparticles as Signal Quenchers

Zhang

et al. 2020

Nanomaterials

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A sensitive fluorescence resonance energy transfer (FRET) biosensor is proposed to detect 8-hydroxy-2′-deoxyguanosine (8-OHdG), which is a typical DNA oxidation damage product excreted in human urine. The FRET biosensor was based on carbon dots (CDs)-modified nanoporous alumina membrane with CDs as fluorescence donors. Gold nanoparticles were encapsulated in zeolitic imidazolate framework-8 to form Au@ZIF-8 nanoparticles as signal quenchers. CDs and Au@ZIF-8 nanoparticles were biofunctionalized by 8-OHdG antibody. The capture of 8-OHdG on the membrane substrates can bring Au@ZIF-8 nanoparticles closely to CDs. With 350 nm excitation, the fluorescence of CDs was quenched by Au@ZIF-8 nanoparticles and FRET effect occurred. The quenching efficiency was analyzed. The limit of detection (LOD) was 0.31 nM. Interference experiments of the FRET biosensor showed good specificity for 8-OHdG detection. The biosensor could detect urinary 8-OHdG sensitively and selectively with simple sample pretreatment processes. It shows applicability for detecting biomarkers of DNA damage in urine or other biological fluids.

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Section: Detection Of 8-ohdg By the Fret Biosensormentioning

confidence: 82%

“…CDs were synthesized with citric acid and EDTA by one-step hydrothermal treatment [ 38 ]. Citric acid (0.22 g) was dissolved in deionized water (DI water, 10 mL).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Sensitive FRET Biosensor Based on Carbon Dots-Modified Nanoporous Membrane for 8-hydroxy-2′-Deoxyguanosine (8-OHdG) Detection with Au@ZIF-8 Nanoparticles as Signal Quenchers

Zhang

et al. 2020

Nanomaterials

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“…Hu et al [36] have developed a fluorometric immunoassay, based on strongly photoluminescent and excellently photostable carbon quantum dots (CQDs) and AuNPs, for determining DNA containing oxidatively damaged o 8 dGuo. The morphology and sizes of AuNPs and CQDs were observed by TEM.…”

Section: Fluorometric Assaysmentioning

confidence: 99%

“…Fluorometric immunoassay using carbon quantum dots (CQDs) for the detection of DNA containing o 8 dGuo lesion[36].…”

mentioning

confidence: 99%

Analytical and Structural Studies for the Investigation of Oxidative Stress in Guanine Oligonucleotides

Ferenc

Váradi

Kupihár

et al. 2020

IJMS

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DNA damage plays a decisive role in epigenetic effects. The detection and analysis of DNA damages, like the most common change of guanine (G) to 8-oxo-7,8-dihydroguanine (OG), is a key factor in cancer research. It is especially true for G quadruplex structure (GQ), which is one of the best-known examples of a non-canonical DNA arrangement. In the present work, we provided an overview on analytical methods in connection with the detection of OG in oligonucleotides with GQ-forming capacity. Focusing on the last five years, novel electrochemical tools, like dedicated electrodes, were overviewed, as well as different optical methods (fluorometric assays, resonance light scattering or UV radiation) along with hyphenated detection and structural analysis methods (CD, NMR, melting temperature analysis and nanopore detection) were also applied for OG detection. Additionally, GQ-related computational simulations were also summarized. All these results emphasize that OG detection and the analysis of the effect of its presence in higher ordered structures like GQ is still a state-of-the-art research line with continuously increasing interest.

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Ultra-sensitive Tb3+/G-quadruplex fluorescence detection of oxidative DNA damage coupled with DNA glycosylase

Wang,

Huang,

Chen

2024

Microchemical Journal

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A carbon dot and gold nanoparticle-based fluorometric immunoassay for 8-hydroxy-2′-deoxyguanosine in oxidatively damaged DNA

Cited by 7 publications

References 42 publications

A Sensitive FRET Biosensor Based on Carbon Dots-Modified Nanoporous Membrane for 8-hydroxy-2′-Deoxyguanosine (8-OHdG) Detection with Au@ZIF-8 Nanoparticles as Signal Quenchers

A Sensitive FRET Biosensor Based on Carbon Dots-Modified Nanoporous Membrane for 8-hydroxy-2′-Deoxyguanosine (8-OHdG) Detection with Au@ZIF-8 Nanoparticles as Signal Quenchers

Analytical and Structural Studies for the Investigation of Oxidative Stress in Guanine Oligonucleotides

Ultra-sensitive Tb3+/G-quadruplex fluorescence detection of oxidative DNA damage coupled with DNA glycosylase

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