A carbon nanotube screen-printed electrode for label-free detection of the human cardiac troponin T

Critical overview on the application of sensors and biosensors for clinical analysis

“…Increased levels of cTnT (higher than 0.3 ng mL −1 ) are highly specific to cardiac injury and are significantly associated with an increased risk of re-infarction and death, which facilities early diagnosis [93]. As shown in Table 2, Fonseca et al [93], Gomes-Filho et al [94], Silva et al [95] and Mattos et al [96] developed immunosensors based on gold nanoparticles, carboxylated CNTs, amine-functionalized CNTs and o-aminobenzoic films, respectively, for immobilizing antibodies specific to cTnT, with an LOD ranging from 0.0015 to 0.033 ng mL −1 . All immunosensors were used for serum samples with high sensitivity.…”

“…They can be used for point-of-care diagnosis of acute myocardial infarction. For example, Gomes-Filho et al [94] and Silva et al [95] compared the cTnT concentrations obtained with both immunosensors in human serum samples to the levels obtained with electrochemiluminescence immunoassays. They [94,95] obtained correlation coefficients of 0.987 and 0.990 between the two methods (p < 0.0001), respectively.…”

Diagnostics on acute myocardial infarction: Cardiac troponin biomarkers

“…To overcome this disadvantage, Silva et al (2013) had developed an amine functionalized CNTs, incorporated into the ink printing used to fabricate screen printed electrode (SPE) to detect cTnT. A better stability in measurement is enabled by incorporating NH 2 -CNT into the carbon ink.…”

Electrochemical biosensors based on nanomodified screen-printed electrodes: Recent applications in clinical analysis

“…Silva et al [39] have developed a carbon nanotube screenprinted electrode for label-free detection of the cardiac troponin T (cTnT), an important marker of acute myocardial infarction. In particular, amine-functionalized CNTs were incorporated into the printing ink used to fabricate SPEs.…”