A cargo-sorting DNA robot

Thubagere, Anupama J.; Li, Wei; Johnson, Robert F.; Chen, Zibo; Doroudi, Shayan; Lee, Yae Lim; Izatt, Gregory; Wittman, Sarah; Srinivas, Niranjan; Woods, Damien; Winfree, Erik; Qian, Lulu

doi:10.1126/science.aan6558

Cited by 480 publications

(454 citation statements)

References 56 publications

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“…This sensitive system could successfully couple payloads with a modification linker and release them in response to different cell types as specific keys [93]. In a recent work, Thubagere et al developed a DNA-based robotic walker for cargo delivery at the molecular level [94]. This robot was able to pick up two types of cargo and deliver them to separate destinations simultaneously by random walking.…”

Section: Cells/microorganisms As Propellersmentioning

confidence: 99%

Micro- and nano-motors: the New Generation of Drug Carriers

2018

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Micro-and nano-motors are emerging as novel drug delivery platforms, offering advantages such as rapid drug transport, high tissue penetration and motion controllability. They can be propelled and/or guided by endogenous (i.e., chemotaxis) or exogenous stimuli (e.g., ultrasound, magnetic fields, light) toward the area of interest. Moreover, such stimuli can be used to trigger the release of a therapeutic payload when the motor reaches certain location in order to improve the drug targeting. In this review article, we highlight medically oriented micro-/nano-motors, in particular the ones created for targeted drug delivery, and discuss their current limitations and possibilities toward in vivo applications. Drug-delivery systems have greatly evolved since 1952, when the first sustained release systems were introduced (see the graphical abstract) [1]. In the 1980s, self-regulated drug release carriers as well as the first drug delivery nanostructures were developed. From then on, research in this field has been focused on improving the targeting and systemic circulation of those carriers as well as to study different nanoparticle-based drug formulations, topics which have been widely reviewed in the literature [2][3][4][5][6][7]. Several nanocarriers (between 5 and 200 nm diameter) have been explored for therapy (e.g. inorganic, polymeric, liposomes, nanocrystals, nanotubes and dendrimers) [8], many of them already approved by the Food and Drug Administration (FDA) to treat, for example, neurovascular diseases, neurological cancers and neurodegenerative maladies [9]. The study of their pharmacokinetics has led to the creation of new carriers with reduced drug dose and protection from efflux or degradation [10]. Most of these nanocarriers include sophisticated surface biofunctionalization and coatings like polyethylene glycol (PEG) [11] or biomimetic modifications, [12] to increase their specificity and circulation time [13]. However, challenges still remain for those carriers such as the improvement of their targeting (currently only about 0.7% -median -of the administrated nanoparticle dose is found to be delivered to a solid tumor [14]), their penetration ability, drug loading capacity and delivery on the subcellular level. Other type of drug carriers are the cellular ones, which have many advantages such Graphical abstract:

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Section: Cells/microorganisms As Propellersmentioning

confidence: 99%

Micro- and nano-motors: the New Generation of Drug Carriers

2018

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Section: The Development Of Dna Nanotechnologymentioning

confidence: 99%

“…The walker can be decorated on DNA origami and moves along the track by consuming the energy from enzyme or strand displacement. So far, DNA walkers were designed to perform simple functions such as walking in a controlled direction (Figure C), or picking up a cargo while walking . Thubagere et al designed a DNA robot that performs a nanomechanical task that is substantially more sophisticated than the previous work.…”

Section: Fabrication Of Dynamic Patterns On Dna Origamimentioning

confidence: 99%

Create Nanoscale Patterns with DNA Origami

Fan

Wang

Kenaan

et al. 2019

Small

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Structural deoxyribonucleic acid (DNA) nanotechnology offers a robust platform for diverse nanoscale shapes that can be used in various applications. Among a wide variety of DNA assembly strategies, DNA origami is the most robust one in constructing custom nanoshapes and exquisite patterns. In this account, the static structural and functional patterns assembled on DNA origami are reviewed, as well as the reconfigurable assembled architectures regulated through dynamic DNA nanotechnology. The fast progress of dynamic DNA origami nanotechnology facilitates the construction of reconfigurable patterns, which can further be used in many applications such as optical/plasmonic sensors, nanophotonic devices, and nanorobotics for numerous different tasks.

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“…More interestingly, Thubagere et al. used three modular building blocks to construct a DNA robot (Figure ) . A single‐stranded DNA is designed as one leg and two foot domains for walking and one arm and one hand domain for picking up and dropping off cargos; thus achieving an algorithm‐encoding recognition between cargos and their destinations.…”

Section: Applications Of D‐crnsmentioning

confidence: 99%

“…Reproduced from ref. with permission. Copyright: 2017 , American Association for the Advancement of Science.…”

Section: Applications Of D‐crnsmentioning

confidence: 99%

DNA‐Based Chemical Reaction Networks

Xiao

Pei

2019

ChemBioChem

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Motivated by complex molecular networks of biological organisms, which enable control of the temporal and spatial concentrations of molecules, the bottom‐up development of artificial chemical reaction networks has received renewed interest from biochemists. Based on hybridization and strand‐displacement reactions, DNA‐based chemical reaction networks (D‐CRNs) provide a promising method to describe and analyze (bio)chemical systems, depending on their high programmability and directionality. Herein, progress in the development of D‐CRNs is discussed, and an overview of significant biochemistry applications based on D‐CRNs reported in recent decades is provided. Furthermore, opportunities and future directions for research into D‐CRNs in biochemistry are also discussed.

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A cargo-sorting DNA robot

Cited by 480 publications

References 56 publications

Micro- and nano-motors: the New Generation of Drug Carriers

Micro- and nano-motors: the New Generation of Drug Carriers

Create Nanoscale Patterns with DNA Origami

DNA‐Based Chemical Reaction Networks

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