A cascade amplification platform assisted with DNAzyme for activity analysis, kinetic study and effector screening of Fpg <i>in vitro</i>

Dang, Wenya; Tong, Chunyi; Yang, Yupei; Liu, Yongbei; Liu, Bin; Zhou, Hongyan; Wang, Wei

doi:10.1039/c8an02253d

Cited by 13 publications

(27 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The next step in our research was the use of δ-lactones as a function of commonly used antibiotics: ciprofloxacin, bleomycin, and cloxacillin a component of the general drug product Syntarpen. Cloxacillin is a beta-lactam antibiotic used to treat bacterial infections, recommended mainly in skin and soft tissue infections, lower respiratory tract infections and osteomyelitis [ 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 ]. Ciprofloxacin is an organic chemical compound, a chemotherapeutic agent from the second-generation quinolone group, with a bactericidal effect, showing its activity by disrupting bacterial DNA replication, inhibiting bacterial DNA topoisomerase and DNA gyrase.…”

Section: Resultsmentioning

confidence: 99%

“…It is the most potent drug among fluoroquinolones. Ciprofloxacin is particularly active against Gram-negative bacteria such as Hemophilus influenzae , Neisseria gonorrhoeae , Salmonella, Shigella , Pseudomonas aeruginosa , Escherichia coli , Staphylococcus aureus , Mycobacterium tuberculosis , Enterobacteriaceae , Bartonella quintana ,), it shows variable activity towards others [ 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 ].…”

Section: Resultsmentioning

confidence: 99%

“…Bleomycin (Latin Bleomycinum) is a glycopeptide antibiotic obtained from the Streptomyces verticillus strain. It is a mixture of polypeptide compounds with cytostatic activity, mainly bleomycin A2 and B2 [ 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 ]. It is a cytostatic antibiotic causing damage to the DNA strand as a result of the binding of the bleomycin–iron ion complex to it, which results in the cleavage of the DNA strand and inhibition of the cell cycle in the G2 and S phase, which in turn leads to the death of the neoplastic cell [ 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 ].…”

Section: Resultsmentioning

confidence: 99%

“…The synthesis of unsaturated secondary alcohols 3 ( Figure 2 ) was carried out following the procedure described by others and Gaylord and Becker [ 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 ]. A solution of aryl bromide 1 (0.08 mol) in diethyl ether ( Figure 2 ) was added dropwise to magnesium turnings (0.08 mol) and heated under reflux for 4 h. The mixture was cooled on ice and a solution of 3-methyl crotonaldehyde 2 in diethyl ether ( Figure 2 ) was added dropwise and stirred for 24 h. The mixture was dissolved in diethyl ether and added to ice and water.…”

Section: Methodsmentioning

confidence: 99%

“…The analysis of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was performed on the basis of the original method described in detail others and by Prost [ 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 ] with its individual modifications for specific compounds analyzed and described in the works [ 5 , 6 , 7 , 8 , 9 ].…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

δ-Lactones—A New Class of Compounds That Are Toxic to E. coli K12 and R2–R4 Strains

et al. 2021

View full text Add to dashboard Cite

Lactones are among the well-known organic substances with a specific taste and smell. They are characterized by antibacterial, antiviral, anti-inflammatory, and anti-cancer properties. In recent years, among this group of compounds, new biologically active substances have been searched by modifying the main (leading) structure with new analogs with stronger or different responses that may have a toxic effect on the cells of pathogenic bacteria and constitute an alternative to commonly used antibiotics. A preliminary study of δ-lactone derivatives as new potential candidates for antibacterial drugs was conducted. Particular emphasis was placed on the selection of the structure of lactones with the highest biological activity, especially those with fluorine in their structure as a substituent in terms of action on bacterial lipopolysaccharide (LPS) in the model strains of Escherichia coli K12 (without LPS in its structure) and R2–R4 (LPS of different lengths in its structure). In the presented studies, on the basis of the conducted MIC and MBC tests, it was shown that the antibacterial (toxic) activity of lactones depends on their structure and the length of the bacterial LPS in the membrane of specific strains. Moreover, oxidative damage of bacterial DNA isolated from bacteria after modification with newly synthesized compounds after application of the repair enzyme Fpg glycosylase was analyzed. The analyzed damage values were compared with the modification with appropriate antibiotics: ciprofloxacin, bleomycin, and cloxacillin. The presented research clearly shows that lactone derivatives can be potential candidates as substitutes for drugs, e.g., the analyzed antibiotics. Their chemical and biological activity is related to coumarin derivatives and the corresponding δ-lactone groups in the structure of the substituent. The observed results are of particular importance in the case of increasing bacterial resistance to various drugs and antibiotics, especially in nosocomial infections and neoplasms, and in the era of a microbial pandemic.

show abstract