A cDNA Clone for Endopolygalacturonase from Apple

Atkinson, Ross G.

doi:10.1104/pp.105.4.1437

Cited by 32 publications

(14 citation statements)

References 6 publications

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“…(a) The amino acid sequences of the polygalacturonase. E. carotovora (Hinton et al, 1990;Saarilahti et al, 1990), P. solanacearum (Huang and Schell, 1990), A. chinensis (Atkinson and Gardner, 1993), M. domestica (Atkinson, 1994), P. americana (Dopico et al, 1993), A. niger (Bussink et al, 1990), A. parasiticus (Cary et al, 1995), A. tubingensis (Ruttowski et al, 1990) a low level of similarity (25-26%) with plant and bacterial Pglp but a higher similarity to fungal Pglp (43-53%).…”

Section: Comparison With Other Sequencesmentioning

confidence: 95%

“…Endopolygalacturonase occurs widely in plants ( Atkinson and Gardner, 1993;Dopico et al, 1993;Atkinson, 1994) and in microorganisms (Bussink et al, 1990;Hinton et al, 1990;Cary et al, 1995;Karam and Belarbi, 1995). It catalyses the hydrolysis of -1,4-glycosidic bonds between two non-methylated galacturonic acid residues in pectic substances.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cloning, sequence analysis and overexpression of aSaccharomyces cerevisiae endopolygalacturonase-encoding gene (PGL1)

Gognies¹,

Gainvors²,

Aigle

et al. 1999

Yeast

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Section: Comparison With Other Sequencesmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Cloning, sequence analysis and overexpression of aSaccharomyces cerevisiae endopolygalacturonase-encoding gene (PGL1)

Gognies¹,

Gainvors²,

Aigle

et al. 1999

Yeast

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“…The box marked with an asterix shows the cystein residue corresponding to amino acid 317 in the tomato fruit PG and the large box shows the conserved region used to identify the rape PCR clones as PGs. The PG protein sequences are from kiwifruit [1], apple [2], peach [20], maize [23], tobacco [33], evening primrose [6l, tomato [14,17], rape [27], alfalfa (EMBL accession number U20431), cotton [15], Arabidopsis (EMBL accession number X72292) and avocado [ 18].…”

Section: Diversity Of Pod Pg Isoforms In Oilseed Rapementioning

confidence: 99%

Isolation and characterisation of a pod dehiscence zone-specific polygalacturonase fromBrassica napus

Petersen¹,

Sander²,

Child

et al. 1996

Plant Mol Biol

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Seven distinct partial cDNAs, similar in sequence to previously described polygalacturonases (PGs), were amplified from cDNA derived from rape pod wall, dehiscence zone and leaves by the polymerase chain reaction. Northern analysis showed that one clone, PG35-8, was expressed at low levels in the dehiscence zone during the first five weeks after anthesis but was very abundantly expressed at week 6. In contrast, no PG35-8-related RNA was detected in the pod wall. Our data suggest that there are temporal and spatial correlations between the breakdown of the middle lamella, of the dehiscence zone cells and the pattern of synthesis of PG35-8 transcripts which may indicate a role for this particular PG in rape pod dehiscence. PG35-8 was used to isolate five cDNA clones from a rape dehiscence zone cDNA library. Restriction enzyme analysis and partial sequencing revealed that they were derived from four highly homologous transcripts which are probably allelic forms of a single gene. One full-length clone, RDPG1, was completely sequenced. The predicted protein of RDPG1 showed its highest identity with PG from apple fruit with an identity of 52%.

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“…Reduction in the levels of a single enzyme often has only minor effects on the maintenance of fruit firmness (Sheehy et al, 1988;Smith et al, 1990). Softening in apples is associated with an increase in the expression of a number of cell wall-related genes such as POLYGALACTURONASE1 (PG1), B-GALACTOSIDASE, and XYLOGLUCAN ENDOTRANSGLYCOSYLASE1 (Atkinson, 1994;Goulao and Oliveira, 2007). The best characterized of these genes is PG1.…”

mentioning

confidence: 99%

The Role of Ethylene and Cold Temperature in the Regulation of the Apple POLYGALACTURONASE1 Gene and Fruit Softening

et al. 2010

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Fruit softening in apple (Malus 3 domestica) is associated with an increase in the ripening hormone ethylene. Here, we show that in cv Royal Gala apples that have the ethylene biosynthetic gene ACC OXIDASE1 suppressed, a cold treatment preconditions the apples to soften independently of added ethylene. When a cold treatment is followed by an ethylene treatment, a more rapid softening occurs than in apples that have not had a cold treatment. Apple fruit softening has been associated with the increase in the expression of cell wall hydrolase genes. One such gene, POLYGALACTURONASE1 (PG1), increases in expression both with ethylene and following a cold treatment. Transcriptional regulation of PG1 through the ethylene pathway is likely to be through an ETHYLENE-INSENSITIVE3-like transcription factor, which increases in expression during apple fruit development and transactivates the PG1 promoter in transient assays in the presence of ethylene. A coldrelated gene that resembles a COLD BINDING FACTOR (CBF) class of gene also transactivates the PG1 promoter. The transactivation by the CBF-like gene is greatly enhanced by the addition of exogenous ethylene. These observations give a possible molecular mechanism for the cold-and ethylene-regulated control of fruit softening and suggest that either these two pathways act independently and synergistically with each other or cold enhances the ethylene response such that background levels of ethylene in the ethylene-suppressed apples is sufficient to induce fruit softening in apples.

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A cDNA Clone for Endopolygalacturonase from Apple

Cited by 32 publications

References 6 publications

Cloning, sequence analysis and overexpression of aSaccharomyces cerevisiae endopolygalacturonase-encoding gene (PGL1)

Cloning, sequence analysis and overexpression of aSaccharomyces cerevisiae endopolygalacturonase-encoding gene (PGL1)

Isolation and characterisation of a pod dehiscence zone-specific polygalacturonase fromBrassica napus

The Role of Ethylene and Cold Temperature in the Regulation of the Apple POLYGALACTURONASE1 Gene and Fruit Softening

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