A cell rolling cytometer reveals the correlation between mesenchymal stem cell dynamic adhesion and differentiation state

Choi, Sungyoung; Levy, Oren; Coelho, Mónica B.; Karp, Jeffrey M.; Karnik, Rohit

doi:10.1039/c3lc50923k

Cited by 26 publications

(20 citation statements)

References 42 publications

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“…The final class of passive sorting devices is comprised of those that contain biomolecular recognition sites to promote cell attachment (59,60) or temporary adhesion (61). Several groups have developed devices that sort cells by the former mechanism (59,62).…”

Section: Leading Microfluidic Sorting Devices Passive Devicesmentioning

confidence: 99%

Translating microfluidics: Cell separation technologies and their barriers to commercialization

Shields

Ohiri

Szott

et al. 2016

Cytometry Part B Clinical

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Advances in microfluidic cell sorting have revolutionized the ways in which cell-containing fluids are processed, now providing performances comparable to, or exceeding, traditional systems, but in a vastly miniaturized format. These technologies exploit a wide variety of physical phenomena to manipulate cells and fluid flow, such as magnetic traps, sound waves and flow-altering micropatterns, and they can evaluate single cells by immobilizing them onto surfaces for chemotherapeutic assessment, encapsulate cells into picoliter droplets for toxicity screenings and examine the interactions between pairs of cells in response to new, experimental drugs. However, despite the massive surge of innovation in these high-performance lab-on-a-chip devices, few have undergone successful commercialization, and no device has been translated to a widely distributed clinical commodity to date. Persistent challenges such as an increasingly saturated patent landscape as well as complex user interfaces are among several factors that may contribute to their slowed progress. In this article, we identify several of the leading microfluidic technologies for sorting cells that are poised for clinical translation; we examine the principal barriers preventing their routine clinical use; finally, we provide a prospectus to elucidate the key criteria that must be met to overcome those barriers. Once established, these tools may soon transform how clinical labs study various ailments and diseases by separating cells for downstream sequencing and enabling other forms of advanced cellular or sub-cellular analysis.

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Section: Leading Microfluidic Sorting Devices Passive Devicesmentioning

confidence: 99%

Translating microfluidics: Cell separation technologies and their barriers to commercialization

Shields

Ohiri

Szott

et al. 2016

Cytometry Part B Clinical

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show abstract

“…8 Also, many studies were successfully conducted in the closed and open systems by using this condition. 9,15,20 The P-selectin solution was injected into the completed PDMS chip using an injection pump, and the chip was incubated at room temperature for three hours. After that, the P-selectin injected chip and a negative control group were washed with 1% BSA (bovine serum albumin).…”

Section: Ligand Coatingmentioning

confidence: 99%

“…To overcome these limitations, a novel cell sorting technique based on cell rolling has been developed. 8,9 Cell rolling is a characteristic phenomenon that occurs as a result of interactions between surface affinities caused by cell receptor-surface ligand bonding and shear fluid flow. Regardless of the cell size, this approach can separate cells using only characteristic surface properties.…”

Section: Introductionmentioning

confidence: 99%

Role of micropillar arrays in cell rolling dynamics

Kim

Koo

Moon

et al. 2017

Analyst

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In this study, we present a role of arrayed micropillar structures in cell rolling dynamics. Cell rolling on a ligand coated surface as a means of cell separation was demonstrated using a micropillar-integrated microfluidic channel. This approach allows the separation of cells according to characteristic surface properties, regardless of cell size. In these experiments, different moving trajectories of the cells between a ligand-coated micropost structure and a 1% BSA coated micropost structure were observed using sequential images. Based on the analysis of the angle of travel of cells in the trajectory, the average angles of travel on the ligand-coated microposts were 1.5° and -3.1° on a 1% BSA-coated micropost structure. The overall force equivalent applied to a cell can be analyzed to predict the cell rolling dynamics when a cell is detached. These results show that it will be possible to design chip geometry for delicate operations and to separate target cells. Furthermore, we believe that these control techniques based on a ligand coated micropillar surface can be used for enhancing cell rolling-based separation in a faster and more continuous manner.

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“…[3][4][5] Many of the microfluidic diagnosis devices involve particle or cell separation or focusing. [6][7][8][9][10][11] A number of techniques have been investigated for such a purpose, e.g., hydrodynamic filtration, [12][13][14] deterministic lateral displacement (DLD), [15][16][17][18][19] hydrophoresis, 20 and inertial microfluidics. 21,22 The performance of these methods is dictated by a lateral length scale of the microchannel, named as critical diameter, in relation of the particle size.…”

Section: Introductionmentioning

confidence: 99%

Making a hydrophoretic focuser tunable using a diaphragm

et al. 2014

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Microfluidic diagnostic devices often require handling particles or cells with different sizes. In this investigation, a tunable hydrophoretic device was developed which consists of a polydimethylsiloxane (PDMS) slab with hydrophoretic channel, a PDMS diaphragm with pressure channel, and a glass slide. The height of the hydrophoretic channel can be tuned simply and reliably by deforming the elastomeric diaphragm with pressure applied on the pressure channel. This operation allows the device to have a large operating range where different particles and complex biological samples can be processed. The focusing performance of this device was tested using blood cells that varied in shape and size. The hydrophoretic channel had a large cross section which enabled a throughput capability for cell focusing of ∼15 000 cells s−1, which was more than the conventional hydrophoretic focusing and dielectrophoresis (DEP)-active hydrophoretic methods. This tunable hydrophoretic focuser can potentially be integrated into advanced lab-on-a-chip bioanalysis devices.

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A cell rolling cytometer reveals the correlation between mesenchymal stem cell dynamic adhesion and differentiation state

Abstract: This communication presents quantitative studies of the dynamic adhesion behavior of mesenchymal stem cells (MSCs) enabled by the combination of cell-surface receptor-ligand interactions and three-dimensional hydrodynamic control by microtopography.

Cited by 26 publications

References 42 publications

Translating microfluidics: Cell separation technologies and their barriers to commercialization

Translating microfluidics: Cell separation technologies and their barriers to commercialization

Role of micropillar arrays in cell rolling dynamics

Making a hydrophoretic focuser tunable using a diaphragm

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