A cerebellar internal model calibrates a feedback controller involved in sensorimotor control

Markov, Daniil A.; Petrucco, Luigi; Kist, Andreas M.; Portugues, Rubén

doi:10.1038/s41467-021-26988-0

Cited by 45 publications

(53 citation statements)

References 79 publications

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“…The chamber was then placed onto the stage of a custom-built lightsheet microscope, previously described in ref. 40 . In brief, light from a 473-nm laser (Cobolt) was scanned with galvanometric mirrors (Sigmann Electronik) horizontally to create a ~5-µm-thick excitation sheet.…”

Section: Methodsmentioning

confidence: 99%

Oligodendrocyte precursor cells sculpt the visual system by regulating axonal remodeling

et al. 2022

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Many oligodendrocyte precursor cells (OPCs) do not differentiate to form myelin, suggesting additional roles of this cell population. The zebrafish optic tectum contains OPCs in regions devoid of myelin. Elimination of these OPCs impaired precise control of retinal ganglion cell axon arbor size during formation and maturation of retinotectal connectivity and degraded functional processing of visual stimuli. Therefore, OPCs fine-tune neural circuits independently of their canonical role to make myelin.

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Section: Methodsmentioning

confidence: 99%

Oligodendrocyte precursor cells sculpt the visual system by regulating axonal remodeling

et al. 2022

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“…The modular architecture of PyZebrascope further enables advanced microscope control and image processing algorithms. For example, a common issue during whole-brain imaging in zebrafish is the sample's slow drift resulting from gravity force, tail motions of unparalyzed fish 14 , or the pressure of pipette attachment during fictive recording in paralyzed fish 11,13,30 . A small amount of drift, especially in the axial direction along which the volumetric scan is undersampled, can result in the loss of neurons during imaging because the neuronal diameter in the zebrafish brain is usually less than 5 μm.…”

Section: Discussionmentioning

confidence: 99%

“…We developed PyZebrascope for our custom-built light-sheet microscope (Fig. 1c and S1, Supplementary Table 1 for parts lists), for which many components are common to those in the previous studies 9,11,14,16 . Our system is equipped with two lasers, providing light sources for two excitation arms that are required to scan fish brains from the lateral and front sides (Fig.…”

Section: Microscope Design and The Architecture Of Pyzebrascopementioning

confidence: 99%

“…These advantages of DSLM are best exploited in the optically transparent brain of larval zebrafish. DSLM enabled studies of whole-brain neural dynamics during visually-evoked swimmming 11,12 , motor learning 13,14 , learned helplessness 15 , threat escape 16 , and body posture change 17 . Usage of the DSLM also revealed spontaneous noise dynamics across the brain 18 and how those dynamics change during neural perturbations 19,20 or administration of psychoactive reagents 21 .…”

Section: Introductionmentioning

confidence: 99%

“…For example, light-sheet microscopes for whole-brain imaging in zebrafish (Fig. 1a) typically consist of two excitation optical paths from the lateral and front sides of the fish and one optical detection path above the fish 11,14,16,17 (Fig. 1b).…”

Section: Introductionmentioning

confidence: 99%

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PyZebraScope: an open-source platform for brain-wide neural activity imaging in zebrafish

Barbara

Kantharaju

Haruvi

et al. 2022

Preprint

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Understanding how neurons interact across the brain to control animal behaviors is one of the central goals in neuroscience. Recent developments in fluorescent microscopy and genetically-encoded calcium indicators led to the establishment of whole-brain imaging methods in zebrafish, which records neural activity across a brain-wide volume with single-cell resolution. Pioneering studies of whole-brain imaging used custom light-sheet microscopes, and their operation relied on commercially developed and maintained software that is not available globally. Hence it has been challenging to disseminate and develop the technology in the research community. Here, we present PyZebrascope, an open-source Python platform designed for neural activity imaging in zebrafish using light-sheet microscopy. PyZebrascope has intuitive user interfaces and implements essential features for whole-brain imaging, such as two orthogonal excitation beams and eye damage prevention. Its modular architecture allows the inclusion of advanced algorithms for microscope control and image processing. As a proof of concept, we implemented an automatic algorithm for maximizing the image resolution in the brain by precisely aligning the excitation beams to the image focal plane. PyZebrascope enables whole-brain neural activity imaging in fish behaving in a virtual reality environment with a stable high data throughput and low CPU and memory consumption. Thus, PyZebrascope will help disseminate and develop light-sheet microscopy techniques in the neuroscience community and advance our understanding of whole-brain neural dynamics during animal behaviors.

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Genetic modeling of degenerative diseases and mechanisms of neuronal regeneration in the zebrafish cerebellum

Namikawa,

Pose-Méndez,

Köster

2024

Cell. Mol. Life Sci.

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The cerebellum is a highly conserved brain compartment of vertebrates. Genetic diseases of the human cerebellum often lead to degeneration of the principal neuron, the Purkinje cell, resulting in locomotive deficits and socio-emotional impairments. Due to its relatively simple but highly conserved neuroanatomy and circuitry, these human diseases can be modeled well in vertebrates amenable for genetic manipulation. In the recent years, cerebellar research in zebrafish has contributed to understanding cerebellum development and function, since zebrafish larvae are not only molecularly tractable, but also accessible for high resolution in vivo imaging due to the transparency of the larvae and the ease of access to the zebrafish cerebellar cortex for microscopy approaches. Therefore, zebrafish is increasingly used for genetic modeling of human cerebellar neurodegenerative diseases and in particular of different types of Spinocerebellar Ataxias (SCAs). These models are well suited to address the underlying pathogenic mechanisms by means of in vivo cell biological studies. Furthermore, accompanying circuitry characterizations, physiological studies and behavioral analysis allow for unraveling molecular, structural and functional relationships. Moreover, unlike in mammals, zebrafish possess an astonishing ability to regenerate neuronal populations and their functional circuitry in the central nervous system including the cerebellum. Understanding the cellular and molecular processes of these regenerative processes could well serve to counteract acute and chronic loss of neurons in humans. Based on the high evolutionary conservation of the cerebellum these regeneration studies in zebrafish promise to open therapeutic avenues for counteracting cerebellar neuronal degeneration. The current review aims to provide an overview over currently existing genetic models of human cerebellar neurodegenerative diseases in zebrafish as well as neuroregeneration studies using the zebrafish cerebellum. Due to this solid foundation in cerebellar disease modeling and neuronal regeneration analysis, the zebrafish promises to become a popular model organism for both unraveling pathogenic mechanisms of human cerebellar diseases and providing entry points for therapeutic neuronal regeneration approaches.

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A cerebellar internal model calibrates a feedback controller involved in sensorimotor control

Cited by 45 publications

References 79 publications

Oligodendrocyte precursor cells sculpt the visual system by regulating axonal remodeling

Oligodendrocyte precursor cells sculpt the visual system by regulating axonal remodeling

PyZebraScope: an open-source platform for brain-wide neural activity imaging in zebrafish

Genetic modeling of degenerative diseases and mechanisms of neuronal regeneration in the zebrafish cerebellum

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