A chemically defined diet enables continuous rearing of the brown planthopper, Nilaparvata lugens(Stal)(Homoptera: Delphacidae).

Abstract

Continuous rearing of the brown planthopper, Nilaparvata lugens (Stål), was achieved on a chemically defined diet D-97 developed in this laboratory. The performance of N. lugens reared on D-97 was much better than that on a previously reported artificial diet MMD-1, although it was inferior to the susceptible rice variety TN1. N. lugens can not finish a complete generation on MMD-1 due to poor fecundity and unhatchability of eggs, but it has been successfully reared for more than 6 generations on D-97. In comparison with TN1-reared N. lugens, D-97-reared insects had a longer nymphal period (more than 3 days longer for males and 5 days longer for females), lighter weight (over 25% lighter for males and 35% lighter for females) and lower fecundity (over 50% less). Of the six generations on D-97, the first two generations had a significantly higher rate of emergence and hatchability. The possible factors influencing rearing efficiency and the potential applications of this diet are also discussed.

Development of an Insect Vector Cell Culture and RNA Interference System To Investigate the Functional Role of Fijivirus Replication Protein

“…A membrane-feeding approach for delivering dsRNAs to WBPHs was used. Second-instar nymphs of WBPH were fed with dsRNAs (0.5 g/l) diluted in an artificial diet, D-97, suitable for rearing planthoppers (8). This mixed diet was held between two layers of stretched parafilm covering one open end of the chamber employed.…”

A Mucin-Like Protein of Planthopper Is Required for Feeding and Induces Immunity Response in Plants

“…To observe the morphology of the salivary sheaths, BPHs were fed on an artificial diet (D-97) consisting of amino acids, vitamins, inorganic salts, and Suc (Fu et al, 2001) using plastic cylinders (4 cm long and 2.5 cm in diameter) as feeding chambers. Sachets formed from two layers of stretched Parafilm M (;4 times the original area), each containing 200-mL portions of D-97, were placed at one end of the chamber.…”

A Salivary Endo-β-1,4-Glucanase Acts as an Effector That Enables the Brown Planthopper to Feed on Rice

“…Stems of rice plants (one plant per pot) were confined individually within glass cylinders as stated above into which 15 third-instar BPH nymphs were released. In the artificial diet experiment, 15 third-instar BPH nymphs were introduced into individual feeding chambers (9 cm long and 2 cm in diameter) as described previously (Fu et al, 2001). The number of surviving BPH nymphs in each cylinder or feeding chamber was recorded every day.…”