A Chemically Functionalized Magnetic Nanoplatform for Rapid and Specific Biomolecular Recognition and Separation

Abstract

We have developed a target-molecule-functionalized magnetic nanoparticle (MNP)-based method to facilitate the study of biomolecular recognition and separation. The superparamagnetic property of MNPs allows the corresponding biomolecules to be rapidly separated from crude biofluids with a significant improvement in recovery yield and specificity. Various MNPs functionalized with tag molecules (chitin, heparin, and amylose) were synthesized for recombinant protein purification, and several probe-functionalized MNPs, such as nitrilotriacetic acid (NTA)@MNP and P(k)@MNP, exhibited excellent extraction efficiency for proteins. In a cell recognition study, mannose-functionalized MNPs allowed specific purification of Escherichia coli with FimH adhesin on the surface. In an immunoprecipitation assay, the antibody-conjugated MNPs reduced the incubation time from 12 to 1 h while maintaining a comparable efficiency. The functionalized MNPs were also used in a membrane proteomic study that utilized the interaction between streptavidin-functionalized MNPs and biotinylated cell membrane proteins. Overall, the functionalized MNPs were demonstrated to be promising probes for the specific separation of targets from proteins to cells and proteomics.

Recent advances in biosensing using magnetic glyconanoparticles

“…Pioneering work of El-Boubbou et al [66] and Hatch et al [67] demonstrated that MNPs functionalized with glycoconjugates could be used to isolate and quantify Escherichia coli, reaching higher sensitivity and selectivity than the same MNPs functionalized with antibodies [67]. Since then, some other examples where glycoMNPs are used to detect bacteria can be found in the literature [68]. Parera Pera et al [48] exploited this concept for the detection, for the first time, of a gram-positive pathogen, Streptococcus suis.…”

Clustering of Iron Oxide Nanoparticles with Amphiphilic Invertible Polymer Enhances Uptake and Release of Drugs and MRI Properties

“…Magnetic nanoparticles (NPs) have gained considerable attention in the past two decades. The outburst of research in this area is motivated by their broad technological applications including single‐bit elements in high‐density magnetic data storage arrays, contrast enhancement agents for magnetic resonance imaging (MRI), selective probes for biomolecular interactions, cell sorters, drug delivery, hyperthermia, etc. A promising application of magnetic NPs is as drug carriers for so‐called magnetic drug delivery .…”

Inhibition of the lethality of Shiga-like toxin-1 by functional gold nanoparticles

“…Nanomedicine has become one of the choices for developing new drugs/ therapies. Nanomaterials coated with Gb 3 analogues have been successfully used to effectively target SLT-1B [26][27][28][29]. The beauty of using Gb 3 immobilized nanoparticles (Gb 3 -NPs) instead of Gb 3 analogues alone is that multivalent interactions are involved in the binding between Gb 3 -NPs with SLT-1B, leading to high binding affinity.…”