A Chemically Standardized Extract of <i>Ziziphus jujuba</i> Fruit (Jujube) Stimulates Expressions of Neurotrophic Factors and Anti‐oxidant Enzymes in Cultured Astrocytes

Chen, Jianping; Yan, Artemis Lu; Lam, Kelly Yin Ching; Lam, Candy; Li, Ning; Yao, Ping; Xiong, Aizhen; Dong, Tingxia; Tsim, Karl Wah Keung

doi:10.1002/ptr.5202

Cited by 26 publications

(20 citation statements)

References 16 publications

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“…Calculation was done by using Ct value of 18S of normalize Ct value of target gene in each sample to obtain ΔCt value, which then was used to compare among different samples. PCR products were analyzed by melting curve analysis to confirm specific amplifications [15]. …”

Section: Methodsmentioning

confidence: 99%

Asarone from Acori Tatarinowii Rhizome prevents oxidative stress-induced cell injury in cultured astrocytes: A signaling triggered by Akt activation

et al. 2017

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Acori Tatarinowii Rhizome (ATR; the dried rhizome of Acori tatarinowii Schott) is a well-known herb being used for mental disorder in China and Asia. Volatile oil is considered as the active ingredient of ATR, and asarones account for more than 90% of total volatile oil. Here, the protective effects of ATR oil and asarones, both α-asarone and β-asarone, were probed in cultured rat astrocytes. The cyto-protective effect of ATR oil and asarones against tBHP-induced astrocyte injury was revealed, and additionally ATR oil and asarones reduced the tBHP-induced intracellular reactive oxygen species (ROS) accumulation. In parallel, the activity of anti-oxidant response element (ARE) promoter construct (pARE-Luc), being transfected in cultured astrocytes, was markedly induced by application of ATR oil and asarones. The mRNAs encoding anti-oxidant enzymes, e.g. glutathione S-transferase (GST), glutamate-cysteine ligase modulatory subunit (GCLM), glutamate-cysteine ligase catalytic subunit (GCLC) and NAD(P)H quinone oxidoreductase (NQO1) were induced by ATR oil and asarones in a dose-dependent manner. The ATR oil/asarone-induced gene expression could be mediated by Akt phosphorylation; because the applied LY294002, a phosphoinositide 3-kinase inhibitor, fully abolished the induction. These results demonstrated that α-asarone and β-asarone could account, at least partly, the function of ATR being a Chinese medicinal herb.

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Section: Methodsmentioning

confidence: 99%

Asarone from Acori Tatarinowii Rhizome prevents oxidative stress-induced cell injury in cultured astrocytes: A signaling triggered by Akt activation

et al. 2017

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“…Thus, the upregulation of neurotrophic factors can play a positive role in treating neurological diseases. The effect of jujube on neurotrophic factor expression was investigated in cultured astrocytes [16]. The treatment with jujube water extract stimulated the expression of neurotrophic factors in a dose-dependent manner, having the highest induction of ~100% for NGF, 100% for BDNF, 100% for GDNF, and 50% for NT3.…”

Section: Jujube On Neuron Differentiationmentioning

confidence: 99%

“…Besides, the application of jujube water extract could stimulate the transcriptional activity of CRE. Further studies also showed that pretreatment with H89 significantly blocked jujube-induced neurotrophic factors including NGF, BDNF, and GDNF, indicating the possible involvement of PKA signaling in jujube-induced neurotrophic factor expression [16]. …”

Section: Jujube On Neuron Differentiationmentioning

confidence: 99%

“…Jujube water extracts stimulated the ARE-mediated transcriptional activity, indicating the activation of Nrf2 pathway (Figure 3). Besides, the application of jujube induced the amounts of NQO1, GCLC (catalytic subunit of GCL), GCLM (modifier subunit of GCL), and GST mRNA levels in cultured astrocytes [16]. Jujube was also revealed to protect ischemic damage in gerbil hippocampus via its antioxidant effect, that is, the upregulation of superoxide dismutase (SOD) 1 and reduction of lipid peroxidation [21].…”

Section: Neuroprotection Against Oxidation Insultmentioning

confidence: 99%

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A Review of Dietary Ziziphus jujuba Fruit (Jujube): Developing Health Food Supplements for Brain Protection

Chen

Xiao-yan

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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102

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The fruits of Ziziphus jujuba, known as jujube or Chinese date, are being consumed all around the world because of their health benefits, as both food and herbal medicine. Traditionally, one of the main functions of jujube, as described in herbal medicine, is to benefit our brain by calming down the mind and improving quality of sleep. Here, the activities of jujubes on nervous system are summarized and discussed. Jujube possesses neuroprotective activities, including protecting neuronal cells against neurotoxin stress, stimulating neuronal differentiation, increasing expression of neurotrophic factors, and promoting memory and learning. Flavonoid, cAMP, and jujuboside could be the potential bioactive ingredients to account for the aforesaid biological activities. These findings imply that jujube is a potential candidate for development of health supplements for prevention and/or treatment of neurological diseases.

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“…In our previous studies, we have verified the hematopoietic function of jujube by regulating EPO expression via hypoxia‐inducible factor‐1α in liver cells (Chen et al , ). Thus, the production of EPO will further trigger the formation of RBC.…”

Section: Discussionmentioning

confidence: 99%

Extract of Ziziphus jujuba Fruit (Jujube) Stimulates Expression of Enzymes Responsible for Heme Recycle via Anti-oxidant Response Element in Cultured Murine Macrophages

Chen

Lam

et al. 2015

Phytother. Res.

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Jujube, the fruit of Ziziphus jujuba Mill., is a functional food and commonly used as a health supplement worldwide. To study the beneficial role of jujube in heme iron recycling during erythrophagocytosis, the expression of heme oxygenase-1 (HO-1), biliverdin reductase A and B, and ferroportin were determined in jujube-treated cultured RAW 264.7 macrophages. Application of a chemically standardized jujube water extract in cultured RAW 264.7 cells for 24 h stimulated the expressions of HO-1, biliverdin reductase A, biliverdin reductase B, and ferroportin in a concentration-dependent manner, having the maximal responses from twofolds to threefolds. A plasmid containing anti-oxidant response element, a regulator for HO-1 transcription, was transfected into RAW 264.7 cells. Application of jujube water extract onto the transfected macrophages stimulated the anti-oxidant response element-mediated transcriptional activity by twofolds. These results supported the potential capacity of jujube by regulating expressions of heme iron recycling genes in cultured macrophages.

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A Chemically Standardized Extract of Ziziphus jujuba Fruit (Jujube) Stimulates Expressions of Neurotrophic Factors and Anti‐oxidant Enzymes in Cultured Astrocytes

Cited by 26 publications

References 16 publications

Asarone from Acori Tatarinowii Rhizome prevents oxidative stress-induced cell injury in cultured astrocytes: A signaling triggered by Akt activation

Asarone from Acori Tatarinowii Rhizome prevents oxidative stress-induced cell injury in cultured astrocytes: A signaling triggered by Akt activation

A Review of Dietary Ziziphus jujuba Fruit (Jujube): Developing Health Food Supplements for Brain Protection

Extract of Ziziphus jujuba Fruit (Jujube) Stimulates Expression of Enzymes Responsible for Heme Recycle via Anti-oxidant Response Element in Cultured Murine Macrophages

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