A Cherry Protein and Its Gene, Abundantly Expressed in Ripening Fruit, Have Been Identified as Thaumatin-Like

Fils-Lycaon, Bernard; Wiersma, Paul A.; Eastwell, Kenneth C.; Sáutière, Pierre

doi:10.1104/pp.111.1.269

Cited by 127 publications

(87 citation statements)

References 23 publications

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“…Compared to other protocols developed for RNA extraction from recalcitrant species and/or tissues (Li & Gray 1997, Sanchez & Mariani 2002, Fils-Lycaon et al 1996, MacLean et al 1996, Birtic & Kranner 2006, De Keukeleire et al 2006, Le Provost et al 2007, Fort et al 2008, Wang et al 2008, the presented method combines the advantages of being phenol-free, easily applicable to a wide range of tissues and not requiring ex pensive commercial kits. Moreover, the varying amounts of starting material needed for different tissues (from 20 mg of styles to 1 g of fruit flesh or peel) enabled testing of the protocol's scalability.…”

Section: Discussionmentioning

confidence: 99%

Total RNA extraction from strawberry tree (Arbutus unedo) and several other woody-plants

Zamboni¹,

Pierantoni²,

Franceschi³

2008

iForest

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© iForest -Biogeosciences and Forestry IntroductionRNA extraction from specific plant organs and tissues is a preliminary step for many molecular studies in plant biology. However, isolation of high-quality RNA from some tis sues is often difficult due to the high amounts of polyphenols, polysaccharides and other secondary metabolites they accu mulate. These contaminants tend to co-puri fy and co-precipitate with the RNA in the presence of alcohols, thereby remaining in the final extracts and interfering with such downstream applications as cDNA synthesis, restriction endonuclease enzyme digestion and the establishment of cDNA libraries (Salzman et al. 1999). These problems often occur during RNA extraction from recalcit rant plants and, especially, from reserve-rich organs like fruits and seeds. In addition, the biosynthesis and accumulation of secondary metabolites in plants under biotic and abiotic stress, such as pathogen infection and drought, can be significantly enhanced (Winkel-Shirley 2002, Chaves et al. 2003. While kits supplied by biotechnology com panies extract RNA successfully from many tissues, they proved ineffective on tissues rich in polyphenols or polysaccharides (Kiefer et al. 2000).Several protocols for RNA isolation from tissues of species with high contents of poly phenols or polysaccharides have been repor ted, including methods using soluble polyvinylpyrrolidone and ethanol precipita tion (Salzman et al. 1999), hot borate (Wan & Wilkins 1994), phenol extraction (Kom janc et al. 1999), calcium precipitation (Dal Cin et al. 2005), 2-butoxyethanol (Malnoy et al. 2001, Manning 1991 or cetyl trimethyl ammonium bromide (CTAB) in the extrac tion buffer (Meisel et al. 2005). We tested four of these protocols (Komjanc et al. 1999, Malnoy et al. 2001, Manning 1991, Meisel et al. 2005 and two commercially available RNA extraction kits to secure high-quality RNA in good amounts from an extremely re calcitrant plant such as strawberry tree (Ar butus unedo); the overall results proved un satisfactory for all the methods, although the one described by Meisel et al. gave better results in terms of RNA purity compared with the others. We thus developed and tested a modified protocol that, through a simple optimization of some critical steps, allowed to achieve a great improvement in both RNA yield and purity when extracting from strawberry tree leaves. The effective ness and versatility of this method was sub sequently tested on different tissues (styles, fruit peel, fruit flesh, roots, leaves and seeds) from Pyrus communis, Prunus avium, Prun us persica and Cydonia oblonga, yielding in all cases adequate RNA amounts with a good purity grade. Materials and Methods Plant materialAll plant materials used for RNA extrac tion were grown in the experimental fields of the Department of Fruit Tree and Woody Plant Sciences (Cadriano, Bologna, Italy). The tissues were completely differentiated and healthy. Pear tree styles were harvested immediately before bloom and fruit peel col lected in the course of ripeni...

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Section: Discussionmentioning

confidence: 99%

Total RNA extraction from strawberry tree (Arbutus unedo) and several other woody-plants

Zamboni¹,

Pierantoni²,

Franceschi³

2008

iForest

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“…More recently, the presence of a TL protein has also been noted in ripe cherries, but its organ specificity and exact timing of onset of accumulation were not established (Fils-Lycaon, 1996). In this study we monitored carefully the ripening sequence pattern of grape berry samples.…”

Section: Discussionmentioning

confidence: 99%

“…In view of the demonstrated expression of TL proteins in other fruits at maturity, it would be interesting to examine the relative resistance or susceptibility of these fruits to new infections of known pathogens throughout their maturation to determine how universal this link may be. In this respect, it is interesting to note that Botrytis cinerea, a fungus that readily infects a number of fruits, including grape berries at maturity, has been found to be relatively insensitive to TL proteins when challenged in vitro (Abad et al, 1996;Fils-Lycaon et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of a Fruit-Specific, Thaumatin-Like Protein That Accumulates at Very High Levels in Conjunction with the Onset of Sugar Accumulation and Berry Softening in Grapes

1997

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“…In plants, TLPs are members of an inducible protein cascade that includes 17 families of pathogenesis-related proteins, PR-1 to PR-17. Their synthesis in plants is triggered mainly in response to biotic and abiotic stresses but is also developmentally regulated, particularly during fruit ripening (3). Additionally, TLPs are present constitutively in different plant organs, including grain (4).…”

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confidence: 99%

Redox-dependent interaction between thaumatin-like protein and β-glucan influences malting quality of barley

Singh

Tripathi

Lemaux

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Barley is the cornerstone of the malting and brewing industry. It is known that 250 quantitative trait loci (QTLs) of the grain are associated with 19 malting-quality phenotypes. However, only a few of the contributing genetic components have been identified. One of these, on chromosome 4H, contains a major malting QTL, QTL2, located near the telomeric region that accounts, respectively, for 28.9% and 37.6% of the variation in the β-glucan and extract fractions of malt. In the current study, we dissected the QTL2 region using an expressionand microsynteny-based approach. From a set of 22 expressed sequence tags expressed in seeds at the malting stage, we identified a candidate gene, TLP8 (thaumatin-like protein 8), which was differentially expressed and influenced malting quality. Transcript abundance and protein profiles of TLP8 were studied in different malt and feed varieties using quantitative PCR, immunoblotting, and enzyme-linked immunosorbent assay (ELISA). The experiments demonstrated that TLP8 binds to insoluble (1, 3, 1, 4)-β-D glucan in grain extracts, thereby facilitating the removal of this undesirable polysaccharide during malting. Further, the binding of TLP8 to β-glucan was dependent on redox. These findings represent a stride forward in our understanding of the malting process and provide a foundation for future improvements in the final beermaking process.Hordeum vulgare | malting | β-glucan | quantitative trait loci | thaumatin-like protein

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A Cherry Protein and Its Gene, Abundantly Expressed in Ripening Fruit, Have Been Identified as Thaumatin-Like

Cited by 127 publications

References 23 publications

Total RNA extraction from strawberry tree (Arbutus unedo) and several other woody-plants

Total RNA extraction from strawberry tree (Arbutus unedo) and several other woody-plants

Identification and Characterization of a Fruit-Specific, Thaumatin-Like Protein That Accumulates at Very High Levels in Conjunction with the Onset of Sugar Accumulation and Berry Softening in Grapes

Redox-dependent interaction between thaumatin-like protein and β-glucan influences malting quality of barley

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