A Citrullus colocynthis fruit extract acutely enhances insulin-induced GLUT4 translocation and glucose uptake in adipocytes by increasing PKB phosphorylation

Drissi, Farah; Lahfa, Farid; González, Teresa María Perandones; Peiretti, Franck; Tanti, Jean‐François; Haddad, Mohamed; Fabre, Nicolás; Govers, Roland

doi:10.1016/j.jep.2020.113772

Cited by 15 publications

(10 citation statements)

References 110 publications

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“…The results suggested that treatment with 90 mg/kg of CCAE for 28 days was insufficient to approve the plant's previously reported insulinotropic effect [76][77][78][79] (Fig. 3); instead, an insulin-enhancing activity leading to a promotion of peripheral (adipose and muscle) tissue glucose uptake has been proposed recently [80]. In contrast to the reported potential of C. colocynthis extracts to diminish the formation of glycated hemoglobin [30,81,82], the decrease of HbA1c percentage in the CCAE-treated groups compared to their untreated counterparts was insignificant in the present study (Fig.…”

Section: Discussionmentioning

confidence: 79%

Differential expression of gluconeogenic enzymes in early- and late-stage diabetes: the effect of Citrullus colocynthis (L.) Schrad. Seed extract on hyperglycemia and hyperlipidemia in Wistar-Albino rats model

et al. 2021

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Background The medicinal plant Citrullus colocynthis (L.) Schrad. (C. colocynthis) may benefit patients at different phases of diabetes by attuning to contrasting situations. Our primary objective was to find the mechanism(s) behind the antidiabetic/anti-hyperlipidemic effects of C.colocynthis seed aqueous extract (CCAE) in two different stages of type 2 diabetes (T2D) in rats. Methods Fasting blood sugar (FBS) levels, body weights, and the degree of impaired glucose tolerance (IGT) were measured in healthy nondiabetic control rats (Con), as well as rats with early and late stages of T2D, denoted as ET2D and LT2D, respectively. CCAE was intraperitoneally (IP) injected for 28 days. In the end, the hepatic mRNA expression levels of the following genes were determined by RT-PCR: glucose-6-phosphatase (G6Pase), phosphoenolpyruvate carboxykinase (PEPCK), insulin-dependent sterol regulatory element-binding protein-1c (SREBP-1c), acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), peroxisome proliferator-activated receptor alpha (PPARα), and carnitine palmitoyltransferase I (CPT1). The liver was examined by hematoxylin and eosin (H&E) and Oil-Red O staining. CCAE was partially analyzed by HPLC-DAD. Results ET2D and LT2D were characterized by differentially elevated FBS, deteriorated bodyweight, and significant IGT compared to Con. Hepatosteatoses of varying morphologies and higher hepatic expression of G6Pase than PRPCK in ET2D versus the opposite in LT2D further confirmed the divergent nature of metabolic aberrations. At the end of 28 days, the high levels of FBS, alkaline phosphatase (ALP), triglyceride (TG), urea, hepatic protein carbonyl content (PCC), and alanine and aspartate aminotransferases (AST and ALT, respectively) persisted in untreated LT2D. CCAE ameliorated oxidative stress and upregulated PPARα expression in diabetic groups and Con; it downregulated CPT1 expression in the LT2D group. CCAE’s ability to lower FBS and serum and hepatic TG in both ET2D and LT2D indicated its ability to act via different mechanisms. Ferulic acid (Fer A) and rutin hydrate (RH) were detected in CCAE. Conclusion CCAE lowered the FBS in ET2D via inhibiting the hepatic G6Pase expression (glycogenolysis). In LT2D, CCAE abated sugar levels by diverting PEPCK activity, preferably towards glyceroneogenesis than gluconeogenesis. The preserved triglyceride/fatty acid (TG/FA) cycle, the upregulated PPARα, and the downregulated CPT1 gene expressions reduced serum and hepatic TG.

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Section: Discussionmentioning

confidence: 79%

Differential expression of gluconeogenic enzymes in early- and late-stage diabetes: the effect of Citrullus colocynthis (L.) Schrad. Seed extract on hyperglycemia and hyperlipidemia in Wistar-Albino rats model

et al. 2021

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“…muscle (Kalhotra et al, 2020). Regarding the effect of C. colocynthis on lowering blood glucose, its fruit extract acutely increased insulininduced GLUT4 translocation and glucose uptake into adipocytes by increasing phosphorylation of protein kinase B (Drissi et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Synergistic properties of garlic and Citrullus colocynthis on reproductive injury caused by diabetes in male rats: Structural and molecular evidence

Mohammadzadeh

Gol

2022

Journal of Food Biochemistry

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This study evaluates the synergistic effect of garlic and Citrullus colocynthis on diabetic reproductive damage by suppressing the AGEs/RAGE/Nox‐4 signaling pathway. Thirty‐five male Wistar rats were divided into five groups (n = 7/group): Control, Diabetic, Diabetic+G (Garlic, 1 mL/100 g b.w), Diabetic+C (C. colocynthis, 10 mg/kg b.w) and Diabetic+GC (Garlic, 1 mL/100 g b.w and C. colocynthis, 10 mg/kg b.w) groups. At the end of the experimental period (30 days), in diabetic rats, glucose increased, and body & testis weight, luteinizing hormone (LH) and testosterone levels, and sperm count decreased significantly and histopathological injuries were observed. In addition, they have increased testicular apoptosis and oxidative stress. Also, the mechanism based on advanced glycation end products (AGEs)/receptors for advanced glycation end products (RAGE)/NADPH oxidase‐4 (Nox‐4) was activated in diabetic rats. Separate consumption of garlic and C. colocynthis in Diabetic+G and Diabetic+C groups alleviated the negative adverse effect of diabetes to some extent, but when they were used in the combination form (Diabetic+GC) improvement was profound. Testis histopathology, increased body and testis weight, and enhanced capacity in protecting diabetic reproductive injury was seen. Decreases in testosterone and LH concentration and sperm count in diabetic rats were also reversed by combined administration of garlic and C. colocynthis. It regulated oxidative stress markers, meanwhile reducing caspase‐3 immunoexpression. In addition, overexpression of RAGE, Nox‐4 and nuclear transcription factor‐κB (NF‐κB) was inhibited by the combination of garlic and C. colocynthis. Practical Applications Diabetes mellitus is wide spread all around the world with variety of complications in body including reproductive system in which patients suffer from physical and psychological aspects. Despite many efforts in providing agents for controlling diabetes and its complications, economic conditions of some countries make it difficult for people to provide costly medicine and as a result, they have to bear the complications until they pass away. However, traditional medicine is still finding its way, especially in poor countries with emphasis on medicinal plants. There have been many studies on plants to alleviate diabetes or its side effects. But, using one plant for long term, may be not so effective. Here, we attempted to find whether two plants from two different species can show more efficacy than each one alone. We noticed garlic and Citrullus colocynthis despite having beneficial effects when used alone, they could show synergistic effects in combination.

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“…In addition, the flavonoids and saponins contents in QIg extract play a crucial role in maintaining normal blood glucose level through stimulation of pancreatic β-cells to secrete insulin hormone or abolishing intestinal glucose absorption (26,27). On the other hand, the hypoglycemic effect of QIg extract might be due to alkaloid and saponin contents of QIg extract on stimulating glucose transporter (GLUT4) expression on the plasma membrane and thus enhancing cellular glucose uptake via insulin dependent mechanism (28).…”

Section: Discussionmentioning

confidence: 99%

“…Diabetes mellitus was induced by a single intraperitoneal (IP) injection of freshly prepared Streptozotocin (Sigma-Aldrich, Saint Louis, MO) at a dose of 55 mg/kg b.w. dissolved in normal saline (28). After 72 h of STZ injection, an overnight fast, blood was taken from the tail vein of the rats.…”

Section: Induction Of Diabetesmentioning

confidence: 99%

Comparative Study Between Quercus Infectoria Galls Extract and Glimepiride On Pancreas and Some Blood Parameters in Diabetic Rats

al¹

2022

Bas.J.Vet.Res.

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Diabetes mellitus (DM) is a metabolic disease involving improperly high blood glucose level, hyperlipidemia and hypoinsulinemia. The aim of current study is to assess and compare the influence of Quercus infectoria galls (QIg) extract and glimepiride on pancreatic βcells secretory function and serum lipid profile in diabetic rats. In the present study, thirty six male rats were used and divided into six groups (n=6), including negative control, STZ induced diabetic control, treated diabetic rats with glimepiride (200mg/Kg Bw), and treated diabetic rats with different concentrations of QIg extracts, 200 mg, 400 mg and 800 mg/Kg Bw for 42 days, respectively. At the end of the experiment, all rats were fasted overnight, blood samples were collected for measuring fasting blood glucose, insulin hormone and lipid profile. All rats were then scarified by using chloroform in order to take part of the pancreatic tissue. The results showed a significant decrease in blood glucose, total cholesterol, triglycerides, low density lipoprotein, very low-density lipoprotein, HbA1c% with obvious elevation in serum insulin hormone and high-density lipoprotein in diabetic rats treated with QIg extract and glimepiride compared with diabetic untreated group. In addition, high atherogenic index in diabetic rats was significantly reduced by different concentrations of QIg extract and glimepiride. Histopathological results revealed that there was a significant and dose-dependent morphological restoration in the pancreatic structure damages especially at high dose of the extract. These findings provide a new insight into the role of QIg methanolic extract in maintaining normal blood glucose and adequate pancreatic insulin hormone secretion in diabetic rats, amelioration of dyslipidemia and associated cardiovascular alterations with subsequent improvement in pancreatic structural damages in diabetic rats.

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A Citrullus colocynthis fruit extract acutely enhances insulin-induced GLUT4 translocation and glucose uptake in adipocytes by increasing PKB phosphorylation

Cited by 15 publications

References 110 publications

Differential expression of gluconeogenic enzymes in early- and late-stage diabetes: the effect of Citrullus colocynthis (L.) Schrad. Seed extract on hyperglycemia and hyperlipidemia in Wistar-Albino rats model

Differential expression of gluconeogenic enzymes in early- and late-stage diabetes: the effect of Citrullus colocynthis (L.) Schrad. Seed extract on hyperglycemia and hyperlipidemia in Wistar-Albino rats model

Synergistic properties of garlic and Citrullus colocynthis on reproductive injury caused by diabetes in male rats: Structural and molecular evidence

Comparative Study Between Quercus Infectoria Galls Extract and Glimepiride On Pancreas and Some Blood Parameters in Diabetic Rats

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