A class of highly polymorphic tetranucleotide repeats for canine genetic mapping

Francisco, Leigh V.; Langston, Amelia; Mellersh, Cathryn S.; Neal, Cassandra L.; Ostrander, Elaine A.

doi:10.1007/s003359900104

Cited by 291 publications

(216 citation statements)

References 20 publications

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“…We analysed a 257 bp fragment of the mtDNA control region and 14 microsatellite loci: FH2001, FH2010, FH2017, FH2054, FH2079, FH2088, FH2096 (Francisco et al 1996), C213, C250, C253, C436, C466, C642 (Ostrander et al 1993), AHT130 (Holmes et al 1995) and VWF (Shibuya et al 1994). Laboratory protocols were as described by Pilot et al (2006).…”

Section: Laboratory Procedures and Data Analysismentioning

confidence: 99%

Unregulated hunting and genetic recovery from a severe population decline: the cautionary case of Bulgarian wolves

et al. 2013

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European wolf (Canis lupus) populations have suffered extensive decline and range contraction due to anthropogenic culling. In Bulgaria, although wolves are still recovering from a severe demographic bottleneck in the 1970s, hunting is allowed with few constraints. A recent increase in hunting pressure has raised concerns regarding long-term viability. We thus carried out a comprehensive conservation genetic analysis using microsatellite and mtDNA markers. Our results showed high heterozygosity levels (0.654, SE 0.031) and weak genetic bottleneck signals, suggesting good recovery since the 1970s decline. However, we found high levels of inbreeding (F IS = 0.113, SE 0.019) and a N e /N ratio lower than expected for an undisturbed wolf population (0.11, 95 % CI 0.08-0.29). We also found evidence for hybridisation and introgression from feral dogs (C. familiaris) in 10 out of 92 wolves (9.8 %). Our results also suggest admixture between wolves and local populations of golden jackals (C. aureus), but less extensive as compared with the admixture with dogs. We detected local population structure that may be explained by fragmentation patterns during the 1970s decline and differences in local ecological characteristics, with more extensive sampling needed to assess further population substructure. We conclude that high levels of inbreeding and hybridisation with other canid species, which likely result from unregulated hunting, may compromise long-term viability of this population despite its current high genetic diversity. The existence of population subdivision warrants an assessment of whether separate management units are needed for different subpopulations. Our study highlights conservation threats for populations with growing numbers but subject to unregulated hunting.

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Section: Laboratory Procedures and Data Analysismentioning

confidence: 99%

Unregulated hunting and genetic recovery from a severe population decline: the cautionary case of Bulgarian wolves

et al. 2013

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“…Microsatellite markers are currently the markers of choice for molecular genetic studies such as reconstruction of phylogenetic and relationships among populations (Bowcock et al, 1994;Forbes et al, 1995;MacHugh et al, 1997), determination of paternity and kinship analyses (Glowatzki-Mullis et al, 1995;Heyen et al, 1997;Luikart et al, 1999;Schlotterer, 2004) forensic studies (Edwards et al, 1992), linkage analysis (Francisco et al, 1996;Kappes et al, 1997;Mellersh et al, 1997), population structures (Arora and Bhatia, 2004;Bruford and Wayne, 1993).…”

Section: Analysis Of Genetic Variation In Animalsmentioning

confidence: 99%

Genetic Characterization of Albanian Sheep Breeds by Microsatellite Markers

Hoda¹,

Marsan²

2012

Analysis of Genetic Variation in Animals

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“…Fourteen autosomal microsatellites, originally developed for dogs, were amplified: c2001, c2006, c2010, c2017, c2054, c2079, c2088 and c2096 (Francisco et al 1996), u250 and u253 (Ostrander etal. 1993), vWF (Shibuya etal.…”

Section: Molecular Analysesmentioning

confidence: 99%

Differentiation of tundra/taiga and boreal coniferous forest wolves: genetics, coat colour and association with migratory caribou

et al. 2007

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The grey wolf has one of the largest historic distributions of any terrestrial mammal and can disperse over great distances across imposing topographic barriers. As a result, geographical distance and physical obstacles to dispersal may not be consequential factors in the evolutionary divergence of wolf populations. However, recent studies suggest ecological features can constrain gene flow. We tested whether wolf-prey associations in uninterrupted tundra and forested regions of Canada explained differences in migratory behaviour, genetics, and coat colour of wolves. Satellite-telemetry data demonstrated that tundra wolves (n = 19) migrate annually with caribou (« = 19) from denning areas in the tundra to wintering areas south of the treeline. In contrast, nearby boreal coniferous forest wolves are territorial and associated year round with resident prey. Spatially explicit analysis of 14 autosomal microsatellite loci (n = 404 individuals) found two genetic clusters corresponding to tundra vs. boreal coniferous forest wolves. A sex bias in gene flow was inferred based on higher levels of mtDNA divergence (Fg^ = 0.282, 0.028 and 0.033; P < 0.0001 for mitochondrial, nuclear autosomal and Y-chromosome markers, respectively). Phenotypic differentiation was substantial as 93% of wolves from tundra populations exhibited light colouration whereas only 38% of boreal coniferous forest wolves did (y} = 64.52, P < 0.0001). The sharp boundary representing this discontinuity was the southern limit of the caribou migration. These findings show that substantial genetic and phenotypic differentiation in highly mobile mammals can be caused by prey-habitat specialization rather than distance or topographic barriers. The presence of a distinct wolf ecotype in the tundra of North America highlights the need to preserve migratory populations.

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A class of highly polymorphic tetranucleotide repeats for canine genetic mapping

Cited by 291 publications

References 20 publications

Unregulated hunting and genetic recovery from a severe population decline: the cautionary case of Bulgarian wolves

Unregulated hunting and genetic recovery from a severe population decline: the cautionary case of Bulgarian wolves

Genetic Characterization of Albanian Sheep Breeds by Microsatellite Markers

Differentiation of tundra/taiga and boreal coniferous forest wolves: genetics, coat colour and association with migratory caribou

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