A Clearing Technique for the Examination of Fungi in Plant Tissue

Hering, T. F.; Nicholson, P. B.

doi:10.1038/201942a0

Cited by 18 publications

(5 citation statements)

References 2 publications

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“…Per genotype three plants were sampled. The sampled leaf segments were fixed in acetic-acid ethanol (1:3, v:v) and stained with 0.03 % trypan blue in lactophenol/ ethanol as described by Hering and Nicholson (1964).…”

Section: Histological Analysismentioning

confidence: 99%

Activation tagging of ATHB13 in Arabidopsis thaliana confers broad-spectrum disease resistance

et al. 2014

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Powdery mildew species Oidium neolycopersici (On) can cause serious yield losses in tomato production worldwide. Besides on tomato, On is able to grow and reproduce on Arabidopsis. In this study we screened a collection of activation-tagged Arabidopsis mutants and identified one mutant, 3221, which displayed resistance to On, and in addition showed a reduced stature and serrated leaves. Additional disease tests demonstrated that the 3221 mutant exhibited resistance to downy mildew (Hyaloperonospora arabidopsidis) and green peach aphid (Myzus persicae), but retained susceptibility to bacterial pathogen Pseudomonas syringae pv tomato DC3000. The resistance trait and morphological alteration were mutually linked in 3221. Identification of the activation tag insertion site and microarray analysis revealed that ATHB13, a homeodomain-leucine zipper (HD-Zip) transcription factor, was constitutively overexpressed in 3221. Silencing of ATHB13 in 3221 resulted in the loss of both the morphological alteration and resistance, whereas overexpression of the cloned ATHB13 in Col-0 and Col-eds1-2 backgrounds resulted in morphological alteration and resistance. Microarray analysis further revealed that overexpression of ATHB13 influenced the expression of a large number of genes. Previously, it was reported that ATHB13-overexpressing lines conferred tolerance to abiotic stress. Together with our results, it appears that ATHB13 is involved in the crosstalk between abiotic and biotic stress resistance pathways.

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Section: Histological Analysismentioning

confidence: 99%

Activation tagging of ATHB13 in Arabidopsis thaliana confers broad-spectrum disease resistance

et al. 2014

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“…For 6 dpi, two homozygous plants for the Sha allele were included. The sampled leaf segments were fixed in acetic acid–ethanol (1:3, v/v) and stained with 0.03% trypan blue in lactophenol–ethanol, as described by Hering and Nicholson ().…”

Section: Methodsmentioning

confidence: 99%

Natural loss‐of‐function mutation of EDR1 conferring resistance to tomato powdery mildew in Arabidopsis thaliana accession C24

Gao

Appiano

Huibers

et al. 2014

Molecular Plant Pathology

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SUMMARYTo screen for potentially novel types of resistance to tomato powdery mildew Oidium neolycopersici, a disease assay was performed on 123 Arabidopsis thaliana accessions. Forty accessions were fully resistant, and one, C24, was analysed in detail. By quantitative trait locus (QTL) analysis of an F2 population derived from C24 × Sha (susceptible accession), two QTLs associated with resistance were identified in C24. Fine mapping of QTL-1 on chromosome 1 delimited the region to an interval of 58 kb encompassing 15 candidate genes. One of these was Enhanced Disease Resistance 1 (EDR1). Evaluation of the previously obtained edr1 mutant of Arabidopsis accession Col-0, which was identified because of its resistance to powdery mildew Golovinomyces cichoracearum, showed that it also displayed resistance to O. neolycopersici. Sequencing of EDR1 in our C24 germplasm (referred to as C24-W) revealed two missing nucleotides in the second exon of EDR1 resulting in a premature stop codon. Remarkably, C24 obtained from other laboratories does not contain the EDR1 mutation. To verify the identity of C24-W, a DNA region containing a single nucleotide polymorphism (SNP) unique to C24 was sequenced showing that C24-W contains the C24-specific nucleotide. C24-W showed enhanced resistance to O. neolycopersici compared with C24 not containing the edr1 mutation. Furthermore, C24-W displayed a dwarf phenotype, which was not associated with the mutation in EDR1 and was not caused by the differential accumulation of pathogenesis-related genes. In conclusion, we identified a natural edr1 mutant in the background of C24.

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“…ex Fr..) TuL). Lesions were either examined fresh or cleared with iactophenol ia 95 per cent alcohol and stained in acid fuchsin or trypan blue, according to the method of Hering and Nicholson (1964).…”

Section: Methodsmentioning

confidence: 99%

Black Speck on Cabbage in Kent

Cook

1976

Plant Pathology

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SUMMARY Black speck is a non‐parasitic disease of cabbage in Florida, U.S.A.; similar symptoms have been studied, on non‐hearting cabbage for antigun cutting in Kent. Lesions are usually black, 0.1–2.0 mm diameter, often with a chlorotic halo. Development of individual lesions starts with necrosis of the stomatal guard cells. No pathogen has been consistently isolated from lesions although Stemphylium spp. and Pseudomonas viridiflava were frequently obtained. The lesions are believed to be physiological in origin. Grateful thanks are due to several farmers in north and east Kent for their help and cooperation in these observations, and also to many A.D.A.S. colleagues, in particular Miss L. A. E. Baker, Miss M. Bateson and Mrs. M. A. Carter, for technical assistance. Thanks are also due to staff at the National Vegetable Research Station, Wellesboume, and Asmer Seeds Ltd.

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A Clearing Technique for the Examination of Fungi in Plant Tissue

Cited by 18 publications

References 2 publications

Activation tagging of ATHB13 in Arabidopsis thaliana confers broad-spectrum disease resistance

Activation tagging of ATHB13 in Arabidopsis thaliana confers broad-spectrum disease resistance

Natural loss‐of‐function mutation of EDR1 conferring resistance to tomato powdery mildew in Arabidopsis thaliana accession C24

Black Speck on Cabbage in Kent

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